1887

Abstract

A novel Gram-stain-negative, agarolytic, rod-shaped, non-flagellated, strictly aerobic strain with gliding motility, designated RZW4-3-2, was isolated from coastal seawater of the Yellow Sea in China (119.625° E 35.517° N). The organism grew optimally at 28 °C, at pH 7.0 and in the presence of 2.0–3.0 % (w/v) NaCl. The strain required seawater or artificial seawater for growth, and NaCl alone did not support growth. Strain RZW4-3-2 contained MK-6 as the major respiratory quinone and iso-C 3-OH, iso-C, iso-C G and iso-C 3-OH as the dominant fatty acids. The polar lipids of strain RZW4-3-2 comprised phosphatidylethanolamine, five unknown phospholipids and one unknown lipid. The DNA G+C content of strain RZW4-3-2 was 32.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain was related most closely to SW150, JCM 15542 and LMG 22569 with 98.1, 97.4 and 97.1 % sequence similarities, respectively. The estimated DNA–DNA hybridization values were 20.00±2.31 %, 20.70±2.33 % and 20.70±2.32 % between strain RZW4-3-2 and SW150, JCM 15542 and LMG 22569, respectively. On the basis of results of polyphasic analyses, strain RZW4-3-2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RZW4-3-2 (=JCM 30824=MCCC 1K00698).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001209
2016-09-01
2021-07-30
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/9/3406.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001209&mimeType=html&fmt=ahah

References

  1. Auch A. F., Klenk H. P., Göker M. 2010a; Standard operating procedure for calculating genome-to-genome distances based on high-scoring segment pairs. Stand Genomic Sci 2:142–148 [View Article]
    [Google Scholar]
  2. Auch A. F., von Jan M., Klenk H. P., Göker M. 2010b; Digital DNA-DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison. Stand Genomic Sci 2:117–134 [View Article]
    [Google Scholar]
  3. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. 1995 Short Protocols in Molecular Biology: A Compendium of Methods From Current Protocols in Molecular Biology, 3rd edn. New York: Wiley;
    [Google Scholar]
  4. Bernardet J. F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium andCytophaga-like bacteria of the International Committee on Systematics of Prokaryotes 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [View Article][PubMed]
    [Google Scholar]
  5. Beveridge T. J., Lawrence J. R., Murray R. G. E. 2007; Sampling and staining for light microscopy. In Methods for General and Molecular Microbiology, 3rd edn. pp. 19–33 Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  6. Breznak J. A., Costilow R. N. 1994; Physicochemical factors in growth. In Methods for General and Molecular Bacteriology , pp. 137–154 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  7. Chen W. M., Sheu F. S., Sheu S. Y. 2012; Aquimarina salinaria sp. nov., a novel algicidal bacterium isolated from a saltpan. Arch Microbiol 194:103–112 [View Article][PubMed]
    [Google Scholar]
  8. Collins M. D. 1994; Isoprenoid quinones. In Chemical Methods in Prokaryotic Systematics , pp. 265–309 Edited by Goodfellow M., O’Donnell A. G. Chichester: Wiley;
    [Google Scholar]
  9. Hsu S. C., Lockwood J. L. 1975; Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. Appl Microbiol 29:422–426[PubMed]
    [Google Scholar]
  10. Kennedy J., Margassery L. M., O'Leary N. D., O'Gara F., Morrissey J., Dobson A. D. 2014; Aquimarina amphilecti sp. nov., isolated from the spongeAmphilectus fucorum . Int J Syst Evol Microbiol 64:501–505 [View Article][PubMed]
    [Google Scholar]
  11. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  12. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120[PubMed] [CrossRef]
    [Google Scholar]
  13. Lewin R. A. 1969; A classification of flexibacteria. J Gen Microbiol 58:189–206 [View Article][PubMed]
    [Google Scholar]
  14. Li G., Lai Q., Sun F., Liu X., Xie Y., Du Y., Li G., Shao Z. 2014a; Aquimarina atlantica sp. nov., isolated from surface seawater of the Atlantic Ocean. Antonie Van Leeuwenhoek 106:293–300 [View Article]
    [Google Scholar]
  15. Li X., Wang L., Huang H., Lai Q., Shao Z. 2014b; Aquimarina penaei sp. nov., isolated from intestinal tract contents of Pacific white shrimp,Penaeus vannamei . Antonie Van Leeuwenhoek 106:1223–1229 [View Article]
    [Google Scholar]
  16. Lin B., Lu G., Zheng Y., Xie W., Li S., Hu Z. 2012; Aquimarina agarilytica sp. nov., an agarolytic species isolated from a red alga. Int J Syst Evol Microbiol 62:869–873 [View Article][PubMed]
    [Google Scholar]
  17. Lyman J., Fleming R. H. 1940; Composition of seawater. J Mar Res 3:134–146
    [Google Scholar]
  18. Meier-Kolthoff J. P., Auch A. F., Klenk H. P., Göker M. 2013; Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 14:60 [View Article][PubMed]
    [Google Scholar]
  19. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  20. Miyazaki M., Nagano Y., Fujiwara Y., Hatada Y., Nogi Y. 2010; Aquimarina macrocephali sp. nov., isolated from sediment adjacent to sperm whale carcasses. Int J Syst Evol Microbiol 60:2298–2302 [View Article][PubMed]
    [Google Scholar]
  21. Nedashkovskaya O. I., Kim S. B., Lysenko A. M., Frolova G. M., Mikhailov V. V., Lee K. H., Bae K. S. 2005; Description of Aquimarina muelleri gen. nov., sp. nov., and proposal of the reclassification of [Cytophaga] latercula Lewin 1969 asStanierella latercula gen. nov., comb. nov. Int J Syst Evol Microbiol 55:225–229 [View Article][PubMed]
    [Google Scholar]
  22. Nedashkovskaya O. I., Vancanneyt M., Christiaens L., Kalinovskaya N. I., Mikhailov V. V., Swings J. 2006; Aquimarina intermedia sp. nov., reclassification of Stanierella latercula (Lewin 1969) as Aquimarina latercula comb. nov. and Gaetbulimicrobium brevivitae Yoon et al. 2006 as Aquimarina brevivitae comb. nov. and emended description of the genus Aquimarina . Int J Syst Evol Microbiol 56:2037–2204 [View Article][PubMed]
    [Google Scholar]
  23. Oh Y. S., Kahng H. Y., Lee Y. S., Yoon B. J., Lim S. B., Jung J. S., Oh D. C., Lee D. H. 2010; Aquimarina litoralis sp. nov., isolated from a coastal seawater. J Microbiol 48:433–437 [View Article][PubMed]
    [Google Scholar]
  24. Park S. C., Choe H. N., Baik K. S., Seong C. N. 2012; Aquimarina mytili sp. nov., isolated from the gut microflora of a mussel,Mytilus coruscus, and emended description ofAquimarina macrocephali . Int J Syst Evol Microbiol 62:1974–1979 [View Article][PubMed]
    [Google Scholar]
  25. Park S. C., Choe H. N., Baik K. S., Seong C. N. 2013; Aquimarina gracilis sp. nov., isolated from the gut microflora of a mussel,Mytilus coruscus, and emended description ofAquimarina spongiae . Int J Syst Evol Microbiol 63:1782–1787 [View Article][PubMed]
    [Google Scholar]
  26. Sasser M. 1990 Identification of Bacteria by Gas Chromatography of Cellular FattyAcids MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  27. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011; mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  28. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  29. Tindall B. J., Sikorski J., Smibert R. M., Krieg N. R. 2007; Phenotypic characterization and the principles of comparative systematics. In Methods for General and Molecular Microbiology, 3rd edn. pp. 330–393 Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R. Washington DC: American Society for Microbiology;
    [Google Scholar]
  30. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. 1987; International committee on systematic bacteriology. report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  31. Yi H., Chun J. 2011; Aquimarina addita sp. nov., isolated from seawater. Int J Syst Evol Microbiol 61:2445–2449 [View Article][PubMed]
    [Google Scholar]
  32. Yoon J. H., Kang S. J., Jung S. Y., Oh H. W., Oh T. K. 2006; Gaetbulimicrobium brevivitae gen. nov., sp. nov., a novel member of the familyFlavobacteriaceae isolated from a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 56:115–119 [View Article][PubMed]
    [Google Scholar]
  33. Yoon B. J., You H. S., Lee D. H., Oh D. C. 2011; Aquimarina spongiae sp. nov., isolated from marine spongeHalichondria oshoro . Int J Syst Evol Microbiol 61:417–421 [View Article][PubMed]
    [Google Scholar]
  34. Yu T., Yin Q., Song X., Zhao R., Shi X., Zhang X. H. 2013; Aquimarina longa sp. nov., isolated from seawater, and emended description ofAquimarina muelleri . Int J Syst Evol Microbiol 63:1235–1240 [View Article][PubMed]
    [Google Scholar]
  35. Yu T., Zhang Z., Fan X., Shi X., Zhang X. H. 2014; Aquimarina megaterium sp. nov., isolated from seawater. Int J Syst Evol Microbiol 64:122–127 [View Article][PubMed]
    [Google Scholar]
  36. Zhang Z., Yu T., Xu T., Zhang X. H. 2014; Aquimarina pacifica sp. nov., isolated from seawater. Int J Syst Evol Microbiol 64:1991–1997 [View Article][PubMed]
    [Google Scholar]
  37. Zhou Y. X., Wang C., Du Z. J., Chen G. J. 2015; Aquimarina agarivorans sp. nov., a genome-sequenced member of the classFlavobacteriia isolated fromGelidium amansii . Int J Syst Evol Microbiol 65:2684–2688 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001209
Loading
/content/journal/ijsem/10.1099/ijsem.0.001209
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error