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Abstract

A novel Gram-stain-negative, agarolytic, rod-shaped, non-flagellated, strictly aerobic strain with gliding motility, designated RZW4-3-2, was isolated from coastal seawater of the Yellow Sea in China (119.625° E 35.517° N). The organism grew optimally at 28 °C, at pH 7.0 and in the presence of 2.0–3.0 % (w/v) NaCl. The strain required seawater or artificial seawater for growth, and NaCl alone did not support growth. Strain RZW4-3-2 contained MK-6 as the major respiratory quinone and iso-C 3-OH, iso-C, iso-C G and iso-C 3-OH as the dominant fatty acids. The polar lipids of strain RZW4-3-2 comprised phosphatidylethanolamine, five unknown phospholipids and one unknown lipid. The DNA G+C content of strain RZW4-3-2 was 32.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain was related most closely to SW150, JCM 15542 and LMG 22569 with 98.1, 97.4 and 97.1 % sequence similarities, respectively. The estimated DNA–DNA hybridization values were 20.00±2.31 %, 20.70±2.33 % and 20.70±2.32 % between strain RZW4-3-2 and SW150, JCM 15542 and LMG 22569, respectively. On the basis of results of polyphasic analyses, strain RZW4-3-2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RZW4-3-2 (=JCM 30824=MCCC 1K00698).

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2016-09-01
2019-12-13
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