1887

Abstract

A novel bacterium, designated HMF3850, was isolated from freshwater collected from the Gyeong-an wetland, Republic of Korea. Cells were Gram-stain-negative, aerobic, straight rods, non-motile, and oxidase- and catalase-positive. Growth was observed at pH 6.0–8.0 and at 10–30 °C on R2A agar. Comparative analysis of 16S rRNA gene sequences revealed that strain HMF3850 is a member of the genus Emticicia , sharing highest sequence similarities with Emticicia aquatica HMF2925 (96.7 % 16S rRNA gene sequence similarity), Emticicia sediminis JBR12 (95.0 %), Emticicia ginsengisoli Gsoil 085 (94.9 %) and Emticicia oligotrophica DSM 17448 (94.7 %). The major fatty acids were iso-C15: 0, summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c) and C16 : 0. The predominant respiratory quinone was MK-7. The major polar lipids were phosphatidylethanolamine, phosphatidylinositol, one unidentified aminophospholipid, two unidentified aminolipids and eight unidentified polar lipids. The DNA G+C content was 38.5 mol %. On the basis of the evidence presented in this study, strain HMF3850 represents a novel species of the genus Emticicia , for which the name Emticicia paludis sp. nov. is proposed. The type strain is HMF3850 (=KCTC 42851=CECT 9087).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001205
2016-09-01
2019-10-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/9/3383.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001205&mimeType=html&fmt=ahah

References

  1. Brown A. E..( 2007;). Benson’s Microbiological Applications: Laboratory Manual in General Microbiology. New York:: McGraw-Hill;.
    [Google Scholar]
  2. Felsenstein J..( 1981;). Evolutionary trees from DNA sequences: a maximum-likelihood approach. . J Mol Evol 17: 368–376.[PubMed] [CrossRef]
    [Google Scholar]
  3. Fitch W. M..( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Biol 20: 406–416.[CrossRef]
    [Google Scholar]
  4. Gonzalez J. M., Saiz-Jimenez C..( 2002;). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. . Environ Microbiol 4: 770–773.[PubMed] [CrossRef]
    [Google Scholar]
  5. Hucker G. J..( 1921;). A new modification and application of the Gram stain. . J Bacteriol 6: 395–397.[PubMed]
    [Google Scholar]
  6. Joung Y., Seo M. A., Kang H., Kim H., Ahn T. S., Cho J. C., Joh K..( 2015;). Emticicia aquatica sp. nov., a species of the family Cytophagaceae isolated from fresh water. . Int J Syst Evol Microbiol 65: 4358–4362.[CrossRef]
    [Google Scholar]
  7. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al.( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62: 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  8. Kimura M..( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16: 111–120. [CrossRef] [PubMed]
    [Google Scholar]
  9. Lane D. J..( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics , pp. 125–175. Edited by Stackebrandt E., Goodfellow M.. Chichester:: Wiley;.
    [Google Scholar]
  10. Liu Q. M., Ten L. N., Yu H. S., Jin F. X., Im W. T., Lee S. T..( 2008;). Emticicia ginsengisoli sp. nov., a species of the family ‘Flexibacteraceae’ isolated from soil of a ginseng field. . Int J Syst Evol Microbiol 58: 1100–1105. [CrossRef] [PubMed]
    [Google Scholar]
  11. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H..( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2: 233–241.[CrossRef]
    [Google Scholar]
  12. Park S. E., Lee S., Kim H., Ahn T. Y..( 2015;). Emticicia sediminis sp. nov. isolated from sediment of a shallow stream. . Int J Syst Evol Microbiol 65: 2496–2499. [CrossRef] [PubMed]
    [Google Scholar]
  13. Pruesse E., Peplies J., Glöckner F. O..( 2012;). SINA: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. . Bioinformatics 28: 1823–1829. [CrossRef] [PubMed]
    [Google Scholar]
  14. Saha P., Chakrabarti T..( 2006;). Emticicia oligotrophica gen. nov., sp. nov., a new member of the family ‘Flexibacteraceae’, phylum Bacteroidetes. . Int J Syst Evol Microbiol 56: 991–995. [CrossRef] [PubMed]
    [Google Scholar]
  15. Saitou N., Nei M..( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4: 406–425.[PubMed]
    [Google Scholar]
  16. Sasser M..( 1990;). Identification of Bacteria by gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE:: MIDI Inc;.
    [Google Scholar]
  17. Stackebrandt E., Goebel B. M..( 1994;). Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44: 846–849.[CrossRef]
    [Google Scholar]
  18. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S..( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. . Mol Biol Evol 30: 2725–2729. [CrossRef] [PubMed]
    [Google Scholar]
  19. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al.( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37: 463–464.[CrossRef]
    [Google Scholar]
  20. Yassin A. F., Haggenel B., Budzikiewicz H., Schaal K. P..( 1993;). Fatty acid and polar lipid composition of the genus Amycolatopsis: application of fast atom bombardment-mass spectrometry to structure analysis of underivatized phospholipids. . Int J Syst Bacteriol 43: 414–420.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001205
Loading
/content/journal/ijsem/10.1099/ijsem.0.001205
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error