1887

Abstract

A polyphasic taxonomic study was performed on two Gram-positive-staining, anaerobic, pleomorphic, rod-shaped strains isolated from human bone and tissue samples. Sequencing of the 16S rRNA genes revealed that the strains belong to a novel species within the genus , most closely related to subsp. and subsp. with similarity values of 98.4 % and 98.1 %, respectively. In addition, protein-coding genes for , clearly separated the novel organism from all species and subspecies of the genus . However, a DNA–DNA hybridization analysis between the novel organism and the type strain ATCC 6919 revealed a value of only 61.1 %. Furthermore, whole genome analysis using the program OrthoANI gave a value of 88.5 %, which is significantly below the cut-off value of 95 % for species delineation. The major fatty acids were iso-C, anteiso-C and iso-C. The DNA G+C content of the type strain was 59.7 mol%. When taken collectively, phenotypic, molecular genetic, chemotaxonomic and phylogenetic information demonstrate that the organism represents a distinct, albeit close relative of On the basis of the results presented, the organism represents a novel member of the genus for which the name sp. nov. is proposed. The type strain is NTS 31307302 (=DSM 29427=CCUG 66358).

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2016-09-01
2020-04-02
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