1887

Abstract

Four yellow-pigmented, Gram-stain-negative, rod-shaped bacteria, strains PP-WC-4G-234, PP-CE-2G-454, PP-WC-1G-202 and PP-CC-3G-650, were isolated from the phyllosphere of The strains shared 99.7–100 % 16S rRNA gene sequence similarity but could be differentiated by genomic fingerprinting using rep- and random amplification of polymorphic DNA PCRs. Phylogenetic analysis based on the 16S rRNA gene placed the strains within the family with highest 16S rRNA gene sequence similarity of 97.2–97.3 % to the type strain of Sequence similarities to all other were below 97 %. The main cellular fatty acids of the strains were C 7 as the predominant fatty acid followed by C and summed feature 3 (C 7/C 8). The polyamine patterns of strains PP-WC-4G-234 and PP-CE-2G-454 contained -homospermidine as a major compound, and the major respiratory quinone was ubiquinone Q-10. Predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, phosphatidylcholine, sulfoquinovosyldiacylglycerol, three unidentified phospholipids and one unidentified lipid only detectable after total lipid staining. The DNA G+C content was 66.4, 68.9, 67.4 and 70.5 mol% for strains PP-WC-4G-234, PP-CE-2G-454, PP-WC-1G-202 and PP-CC-3G-650, respectively. Based on phylogenetic, chemotaxonomic and phenotypic analyses we propose two novel species of the genus sp. nov. with PP-WC-4G-234 (=LMG 28655=CIP 110892) as the type strain and sp. nov. with PP-CE-2G-454 (=LMG 29411=CCM 8665) as the type strain and two further strains representing the same species, PP-WC-1G-202 and PP-CC-3G-650.

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2016-09-01
2020-02-26
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