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Volume 66, Issue 9

, and , three novel species of subdivision 4 (class ) isolated from savanna soil and description of the novel family Free

Abstract

Three novel strains of the phylum (Ac_11_E3, Ac_12_G8 and Ac_16_C4) were isolated from Namibian semiarid savanna soils by a high-throughput cultivation approach using low-nutrient growth media. 16S rRNA gene sequence analysis placed all three strains in the order of the class ( subdivision 4). However, 16S rRNA gene sequence similarities to their closest relative K22 were ≤90 %. Cells of strains Ac_11_E3, Ac_12_G8 and Ac_16_C4 were Gram-staining-negative and non-motile and divided by binary fission. Ac_11_E3 and Ac_16_C4 formed white colonies, while those of Ac_12_G8 were orange-yellowish. All three strains were aerobic chemoorganoheterotrophic mesophiles with a broad pH range for growth. All strains used a very limited spectrum of carbon and energy sources for growth, with a preference for complex proteinaceous substrates. The major respiratory quinone was MK-8. The major shared fatty acid was iso-C. The DNA G+C contents of strains Ac_11_E3, Ac_12_G8 and Ac_16_C4 were 55.9 mol%, 66.9 mol% and 54.7 mol%, respectively. Based on these characteristics, the two novel genera gen. nov. and gen. nov. are proposed, harboring the novel species sp. nov. (Ac_11_E3=DSM 27934=LMG 28618), sp. nov. (Ac_16_C4=DSM 29892=LMG 28995) and sp. nov. (Ac_12_G8=DSM 26556=LMG 29166), respectively. Since these novel genera are only distantly related to established families, we propose the novel family fam. nov. that accommodates the proposed genera and the genus ).

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  • Accepted:
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Keyword(s): Acidobacteria subdivision 4 , Arenimicrobium , Blastocatellia , Brevitalea , Pyrinomonadaceae and semiarid soil
© 2016 IUMS
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2016-09-01
2024-04-25
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Brevitalea aridisoli, B. deliciosa and Arenimicrobium luteum, three novel species of Acidobacteria subdivision 4 (class Blastocatellia) isolated from savanna soil and description of the novel family Pyrinomonadaceae
Int J Syst Evol Microbiol 66, 3355 (2016); https://doi.org/10.1099/ijsem.0.001199
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