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Abstract

A nocardioform strain IFM 11456 was isolated from the aqueous humor from a patient with endophthalmitis and was characterized to its taxonomic position. IFM 11456 contained arabinose, galactose and -diaminopimelic acid in whole-cell hydrolysates and mycolic acids that co-migrated with those from the type strain of . The acyl type of muramic acid was -glycolyl. The diagnostic polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and two unidentified glycolipids and the predominant menaquinone was MK-8(H, ω-cycl.). These characteristics are typical of members of the genus . Results of phylogenetic analyses based on 16S rRNA gene sequences indicated that the isolate represented a novel species of the genus and was most closely related to the type strains of JCM 15508 (98.1 %) and IFM 0372 (98.1 %). However, analysis of partial sequences showed that strain IFM 11456 had 90.2 % similarity to IFM 0354 and 90 % to IFM 0330. The DNA–DNA relatedness values for strain IFM 11456 compared with JCM 15508, IFM 0372 and IFM 0354 ranged from 24.4 to 39.9 %. Phenotypic characteristics that differentiated IFM 11456 from phylogenetically related species were growth at 45 °C, utilization of citrate and growth with inositol as a sole carbon source. On the basis of this polyphasic study, the isolate represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is IFM 11456 (=NBRC 109590=TBRC 5149).

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2016-09-01
2022-01-23
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