RT Journal Article SR Electronic(1) A1 Songsumanus, Apakorn A1 Kudo, Takuji A1 Ohkuma, Moriya A1 Phongsopitanun, Wongsakorn A1 Tanasupawat, SomboonYR 2016 T1 Actinomadura montaniterrae sp. nov., isolated from mountain soil JF International Journal of Systematic and Evolutionary Microbiology, VO 66 IS 9 SP 3310 OP 3316 DO https://doi.org/10.1099/ijsem.0.001196 PB Microbiology Society, SN 1466-5034, AB The taxonomic position of the mountain soil actinomycete, strain CYP1-1BT, was clarified by a polyphasic study. The strain produced a single spore, or occasionally a chain of spores, on aerial mycelium. Chemotaxonomic data supported the classification of CYP1-1BT as representing a member of the genus Actinomadura on the basis of the presence of meso-diaminopimelic acid in the peptidoglycan; galactose, glucose, madurose and ribose as whole cell sugars; MK-9(H6), MK-9(H8) and MK-9(H4) as dominant menaquinones; C16 : 0, 10-methylated C18 : 0 and C18 : 1ω9c as the major cellular fatty acids; and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol manosides as the predominant phospholipids. The DNA G+C content was 74.3 mol%. On the basis of the combination of morphological and chemotaxonomic characteristics, CYP1-1BT was identified as representing a member of the genus Actinomadura . On the basis of the results of 16S rRNA gene analysis, CYP1-1BT, was shown to be closely related to Actinomadura nitritigenes DSM 44137T (98.9 %). Phenotypic, genotypic and DNA–DNA hybridization data supported the hypothesis that CYP1-1BT represents a novel species of the genus Actinomadura for which the name Actinomadura montaniterrae sp. nov. is proposed. The type strain is CYP1-1BT (=JCM 16995T=KCTC 39784T=PCU 349T=TISTR 2400T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001196