1887

Abstract

The taxonomic position of the mountain soil actinomycete, strain CYP1-1B, was clarified by a polyphasic study. The strain produced a single spore, or occasionally a chain of spores, on aerial mycelium. Chemotaxonomic data supported the classification of CYP1-1B as representing a member of the genus Actinomadura on the basis of the presence of meso-diaminopimelic acid in the peptidoglycan; galactose, glucose, madurose and ribose as whole cell sugars; MK-9(H6), MK-9(H8) and MK-9(H4) as dominant menaquinones; C16 : 0, 10-methylated C18 : 0 and C18 : 1ω9c as the major cellular fatty acids; and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol manosides as the predominant phospholipids. The DNA G+C content was 74.3 mol%. On the basis of the combination of morphological and chemotaxonomic characteristics, CYP1-1B was identified as representing a member of the genus Actinomadura . On the basis of the results of 16S rRNA gene analysis, CYP1-1B, was shown to be closely related to Actinomadura nitritigenes DSM 44137 (98.9 %). Phenotypic, genotypic and DNA–DNA hybridization data supported the hypothesis that CYP1-1B represents a novel species of the genus Actinomadura for which the name Actinomadura montaniterrae sp. nov. is proposed. The type strain is CYP1-1B (=JCM 16995=KCTC 39784=PCU 349=TISTR 2400).

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2016-09-01
2019-10-14
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