1887

Abstract

A novel Gram-stain-negative, rod-shaped, gliding, halotolerant, aerobic, light-pink-pigmented bacterium, strain JL3085, was isolated from surface water of the South China Sea (16° 49′ 4″ N 112° 20′ 24″ E; temperature: 28.3 °C, salinity: 34.5%). The major respiratory quinone was menaquinone 7 (MK-7). The polar lipids of strain JL3085 comprised phosphatidylethanolamine, four unidentified phospholipids and three unidentified lipids. The major fatty acids were iso-C, summed feature 3 (comprising iso-C 2-OH and/or C 7), iso-C 3-OH, iso-C 9, C 6, anteiso-C and C 5. The DNA G+C content of strain JL3085 was 43.8 mol%. 16S rRNA gene sequence analysis indicated that strain JL3085 was affiliated with the genus , a member of the phylum , and was related most closely to KMM 6221 (96.8 % similarity). DNA–DNA relatedness between strain JL3085 and KMM 6221 was 27.5 %. Based on the evidence presented here, strain JL3085 is regarded as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JL3085 (=NBRC 111782=CGMCC 1.15407).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001191
2016-09-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/9/3299.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001191&mimeType=html&fmt=ahah

References

  1. Bauer A. W., Kirby W. M. M., Sherris J. C. T., Turck M. 1966; Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45:493–496[PubMed]
    [Google Scholar]
  2. Bowman J. P. 2000; Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50:1861–1868 [View Article][PubMed]
    [Google Scholar]
  3. Cappuccino J. G., Sherman N. 2002 Microbiology: a laboratory manual, 6th edn. Menlo Park, CA: Benjamin/Cummings;
    [Google Scholar]
  4. Dong X.-Z., Cai M.-Y. 2001 Determinative manual for routine bacteriology Peking: Scientific Press;
    [Google Scholar]
  5. Embley T. M. 1991; The linear PCR reaction: a simple and robust method for sequencing amplified rRNA genes. Lett in Appl Microbiol 13:171–174 [View Article][PubMed]
    [Google Scholar]
  6. Fautz E., Reichenbach H. 1980; A simple test for flexirubin-type pigments. FEMS Microbiol Lett 8:87–91 [View Article]
    [Google Scholar]
  7. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. 1994 Methods for general and molecular bacteriology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  8. Huss V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [View Article][PubMed]
    [Google Scholar]
  9. Johnson J. L., Whitman W. B. 1994; Similarity analysis of DNAs. In Methods for General and Molecular Bacteriology pp. 655–681 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  10. Kates M. 1986; Techniques of Lipidology: Isolation, Analysis and Identification of Lipids . Laboratory Techniques in Biochemistry and Molecular Biology, 2nd edn. vol 3 Amsterdam: Elsevier;
    [Google Scholar]
  11. Kim H., Joung Y., Ahn T. S., Joh K. 2011; Echinicola jeungdonensis sp. nov., isolated from a solar saltern. Int J Syst Evol Microbiol 61:2065–2068 [View Article][PubMed]
    [Google Scholar]
  12. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  13. Kuykendall L. D., Roy M. A., O'neill J. J., Devine T. E. 1988; Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of bradyrhizobium japonicum . Int J Syst Evol Microbiol 38:358–361 [View Article]
    [Google Scholar]
  14. Ley J. D., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142[PubMed] [CrossRef]
    [Google Scholar]
  15. Logan N. A., Berge O., Bishop A. H., Busse H. J., De Vos P., Fritze D., Heyndrickx M., Kämpfer P., Rabinovitch L. et al. 2009; Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. Int J Syst Evol Microbiol 59:2114–2121 [View Article][PubMed]
    [Google Scholar]
  16. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  17. Miller L. T. 1982; Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 16:584–586[PubMed]
    [Google Scholar]
  18. Nedashkovskaya O. I., Kim S. B., Vancanneyt M., Lysenko A. M., Shin D. S., Park M. S., Lee K. H., Jung W. J., Kalinovskaya N. I. et al. 2006; Echinicola pacifica gen. nov., sp. nov., a novel flexibacterium isolated from the sea urchin Strongylocentrotus intermedius . Int J Syst Evol Microbiol 56:953–958 [View Article][PubMed]
    [Google Scholar]
  19. Nedashkovskaya O. I., Kim S. B., Hoste B., Shin D. S., Beleneva I. A., Vancanneyt M., Mikhailov V. V. 2007; Echinicola vietnamensis sp. nov., a member of the phylum Bacteroidetes isolated from seawater. Int J Syst Evol Microbiol 57:761–763 [View Article][PubMed]
    [Google Scholar]
  20. Ostle A. G., Holt J. G. 1982; Nile blue A as a fluorescent stain for poly-beta-hydroxybutyrate. Appl Environ Microbiol 44:238–241[PubMed]
    [Google Scholar]
  21. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  22. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Microbiol , pp. 611–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  23. Srinivas T. N. R., Tryambak B. K., Kumar P. A. 2012; Echinicola shivajiensis sp. nov., a novel bacterium of the family Cyclobacteriaceae isolated from brackish water pond. Antonie Van Leeuwenhoek 101:641–647 [View Article][PubMed]
    [Google Scholar]
  24. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011; mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  25. Tindall B. J. 1990a; A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130 [View Article]
    [Google Scholar]
  26. Tindall B. J. 1990b; Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66:199–202 [View Article]
    [Google Scholar]
  27. Tindall B. J., Rosselló-Móra R., Busse H. J., Ludwig W., Kämpfer P. 2010; Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol 60:249–266 [View Article][PubMed]
    [Google Scholar]
  28. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  29. Yurkov V. V., Krieger S., Stackebrandt E., Beatty J. T. 1999; Citromicrobium bathyomarinum, a novel aerobic bacterium isolated from deep-sea hydrothermal vent plume waters that contains photosynthetic pigment-protein complexes. J Bacteriol 181:4517–4525[PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001191
Loading
/content/journal/ijsem/10.1099/ijsem.0.001191
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error