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Abstract

A novel actinomycete strain, designated TRM 46794-61, was isolated from an alkali-removing canal in 14th Farms of Xinjiang Production and Construction Corps, north-west China. The isolate contained -diaminopimelic acid as the diagnostic diamino acid. The whole-cell sugar patterns of the isolate contained ribose, mannose and glucose. The polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, phosphatidylinositol mannoside and two unidentified phospholipids. The predominant menaquinones were MK-9(H), MK-9(H), MK-9(H) and MK-9(H). The major fatty acids were iso-C, anteiso-C and anteiso-C. The G+C content of the DNA was 70.4 mol%. Phylogenetic analysis showed that strain TRM 46794-61 had a 16S rRNA gene sequence similarity of 97.6 % with the most closely related species with a validly published name, TRM 46012, and it could be distinguished from all species in the genus based on data from this polyphasic taxonomic study. However, DNA–DNA hybridization studies between strain TRM 46794-61 and TRM 46012 showed only 45.4 % relatedness. On the basis of these data, strain TRM 46794-61 should be designated as a representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is TRM 46794-61(=CCTCC AA 2015006=KCTC 39568).

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2016-08-01
2020-09-28
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