1887

Abstract

A novel actinomycete strain, designated TRM 46794-61, was isolated from an alkali-removing canal in 14th Farms of Xinjiang Production and Construction Corps, north-west China. The isolate contained -diaminopimelic acid as the diagnostic diamino acid. The whole-cell sugar patterns of the isolate contained ribose, mannose and glucose. The polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, phosphatidylinositol mannoside and two unidentified phospholipids. The predominant menaquinones were MK-9(H), MK-9(H), MK-9(H) and MK-9(H). The major fatty acids were iso-C, anteiso-C and anteiso-C. The G+C content of the DNA was 70.4 mol%. Phylogenetic analysis showed that strain TRM 46794-61 had a 16S rRNA gene sequence similarity of 97.6 % with the most closely related species with a validly published name, TRM 46012, and it could be distinguished from all species in the genus based on data from this polyphasic taxonomic study. However, DNA–DNA hybridization studies between strain TRM 46794-61 and TRM 46012 showed only 45.4 % relatedness. On the basis of these data, strain TRM 46794-61 should be designated as a representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is TRM 46794-61(=CCTCC AA 2015006=KCTC 39568).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001180
2016-08-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/8/3219.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001180&mimeType=html&fmt=ahah

References

  1. Bérdy J. 2012; Thoughts and facts about antibiotics: where we are now and where we are heading. J Antibiot 65:385–395 [View Article][PubMed]
    [Google Scholar]
  2. Chun J., Goodfellow M. 1995; A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45:240–245 [View Article][PubMed]
    [Google Scholar]
  3. Collins M. D. 1985; Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics pp. 267–287 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  4. Felsenstein J. 1985; Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  5. Gebreyohannes G., Moges F., Sahile S., Raja N. 2013; Isolation and characterization of potential antibiotic producing actinomycetes from water and sediments of Lake Tana, Ethiopia. Asian Pac J Trop Med 3:426–435 [CrossRef]
    [Google Scholar]
  6. Groth I., Schumann P., Rainey F. A., Martin K., Schuetze B., Augsten K. 1997; Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 47:1129–1133 [View Article][PubMed]
    [Google Scholar]
  7. Han X. X., Luo X. X., Zhang L. L. 2014; Glycomyces fuscus sp. nov. and Glycomyces albus sp. nov., actinomycetes isolated from a hypersaline habitat. Int J Syst Evol Microbiol 64:2437–2441 [View Article][PubMed]
    [Google Scholar]
  8. Hasegawa T., Takizawa M., Tanida S. 1983; A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–322 [CrossRef]
    [Google Scholar]
  9. He L., Li W., Huang Y., Wang L., Liu Z., Lanoot B., Vancanneyt M., Swings J. 2005; Streptomyces jietaisiensis sp. nov., isolated from soil in northern China. Int J Syst Evol Microbiol 55:1939–1944 [View Article][PubMed]
    [Google Scholar]
  10. Kelly K. L. 1964 Inter-Society Color Council-National Bureau of Standards Color Name Charts Illustrated with Centroid Colors Washington, DC: US Government Printing Office;
    [Google Scholar]
  11. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  12. Li W. J., Zhang Y. G., Zhang Y. Q., Tang S. K., Xu P., Xu L. H., Jiang C. L. 2005; Streptomyces sodiiphilus sp. nov., a novel alkaliphilic actinomycete. Int J Syst Evol Microbiol 55:1329–1333 [View Article][PubMed]
    [Google Scholar]
  13. Liu C. X., Wang X. J., Zhao J. W., Liu Q. F., Wang L., Guan X. J., He H. R., Xiang W. S. 2013; Streptomyces harbinensis sp. nov., an endophytic, ikarugamycin-producing actinomycete isolated from soybean root [Glycine max (L.) Merr.]. Int J Syst Evol Microbiol 63:3579–3584 [View Article][PubMed]
    [Google Scholar]
  14. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  15. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  16. Shirling E. B., Gottlieb D. 1969; Cooperative description of type cultures of Streptomyces. IV. Species descriptions from the second, third and fourth studies. Int J Syst Bacteriol 19:391–512 [View Article]
    [Google Scholar]
  17. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231[PubMed]
    [Google Scholar]
  18. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
    [Google Scholar]
  19. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011; mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  20. Vijayabharathi R., Bruheim P., Andreassen T., Raja D. S., Devi P. B., Sathyabama S., Priyadarisini V. B. 2011; Assessment of resistomycin, as an anticancer compound isolated and characterized from Streptomyces aurantiacus AAA5. J Microbiol 49:920–926 [View Article][PubMed]
    [Google Scholar]
  21. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  22. Williams S. T., Goodfellow M., Alderson G. 1989; Genus Streptomyces Waksman and Henrici 1943, 339AL. In Bergey’s Manual of Systematic Bacteriology vol 4 pp. 2452–2492 Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  23. Xia Z. F., Ruan J. S., Huang Y., Zhang L. L. 2013; Streptomyces aidingensis sp. nov., an actinomycete isolated from lake sediment. Int J Syst Evol Microbiol 63:3204–3208 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001180
Loading
/content/journal/ijsem/10.1099/ijsem.0.001180
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error