1887

Abstract

A novel actinobacterial strain RC1832 was isolated from the sediment of a fish dumping yard at Balugaon near Chilika Lake. The strain is halotolerant (15 % NaCl, w/v), alkali-tolerant (pH 7–10) and hydrolyzes chitin, starch, gelatin, cellulose, carboxymethyl cellulose, Tween 80, tributyrin, lecithin and casein. Apart from showing typical genus-specific morphological and chemotaxonomic features, the comparision and analysis of the near complete 16S rRNA gene sequence clearly revealed that the strain RC1832represented a member of the genus Streptomyces . It exhibited the highest sequence similarities with the strains Streptomyces fenghuangensis GIMN4.003 (99.78 %), Streptomyces nanhaiensis DSM 41926 (99.07 %), Streptomyces radiopugnans R97(98.71 %), Streptomyces atacamensis DSM 42065 (98.65 %) and Streptomyces barkulensis DSM 42082 (98.25 %). The DNA–DNA relatedness of strain RC 1832 with the closest phylogenetic neighbours S. fenghuangensis GIMN4.003 and S. nanhaiensis DSM 41926 were 20±2 % and 21±2 %, respectively. Thus, based on a range of phenotypic and genotypic properties, strain RC1832 was suggested to represent a novel species of the genus Streptomyces for which the name Streptomyces chitinivorans sp. nov. is proposed. The type strain is RC1832 (=JCM 30611=KCTC 29696).

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2016-08-01
2019-10-15
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