1887

Abstract

The novel actinomycete, strain CH3-3, was isolated from mangrove sediment collected from Chonburi Province, Thailand. On the basis of its spore morphology and chemotaxonomic characteristics, the strain belonged to the genus Micromonospora . It contained meso-diaminopimelic acid, glucose, mannose, xylose, ribose and rhamnose in the whole-cell hydrolysate, MK-10(H4), MK-10(H6) and MK-10(H8) as major menaquinones, and iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0 as major cellular fatty acids. blast analysis of 16S rRNA gene sequences revealed that the strain showed highest similarity with Micromonospora palomenae NEAU-CX1(98.97 %) and Micromonospora coxensis 2–30-b(28) (98.97 %). Based on phylogenetic tree analysis of the 16S rRNA gene sequence, the strain formed a cluster with M. palomenae NEAU-CX1, Micromonospora halophytica DSM 43171, M. coxensis 2-30-b(28) and Micromonospora purpureochromogenes DSM 43821. On the basis of phenotypic differences and DNA–DNA relatedness evidence, strain CH3-3 could be clearly distinguished from the closely related species of the genus Micromonospora and represents a novel species of the genus Micromonospora for which the name Micromonospora sediminis sp. nov. is proposed. The type strain is CH3-3 (=JCM 18523=PCU 350=TISTR 2396).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001175
2016-08-01
2019-12-05
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/8/3235.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001175&mimeType=html&fmt=ahah

References

  1. Arai T. . ( 1975;). Culture Media for Actinomycetes. Japan, Tokyo:: The Society for Actinomycetes;.
    [Google Scholar]
  2. Collins M. D. , Pirouz T. , Goodfellow M. , Minnikin D. E. . ( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100: 221–230. [CrossRef] [PubMed]
    [Google Scholar]
  3. Fang B. , Liu C. , Guan X. , Song J. , Zhao J. , Liu H. , Li C. , Ning W. , Wang X. , Xiang W. . ( 2015;). Two new species of the genus Micromonospora: Micromonospora palomenae sp. nov. and Micromonospora harpali sp. nov. isolated from the insects. . Antonie Van Leeuwenhoek 108: 141–150. [CrossRef] [PubMed]
    [Google Scholar]
  4. Felsenstein J. . ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17: 368–376.[PubMed] [CrossRef]
    [Google Scholar]
  5. Felsenstein J. . ( 1983;). Parsimony in systematics: biological and statistical issues. . Annu Rev Ecol Syst 14: 313–333. [CrossRef]
    [Google Scholar]
  6. Felsenstein J. . ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39: 783–791. [CrossRef]
    [Google Scholar]
  7. Garcia L. C. , Martínez-Molina E. , Trujillo M. E. . ( 2010;). Micromonospora pisi sp. nov., isolated from root nodules of Pisum sativum . . Int J Syst Evol Microbiol 60: 331–337. [CrossRef] [PubMed]
    [Google Scholar]
  8. Genilloud O. . ( 2012;). Genus I. Micromonospora Ørskov 1923, 156AL. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn.,Vol 5 , pp. 1039–1056. Edited by Goodfellow M. , Kämpfer P. , Busse M.-J. , Trujillo M. E. , Suzuki K. , Ludwig W. , Whitman W. B. . NewYork:: Springer;.
    [Google Scholar]
  9. Kasai H. , Tamura T. , Harayama S. . ( 2000;). Intrageneric relationships among Micromonospora species deduced from gyrB-based phylogeny and DNA relatedness. . Int J Syst Evol Microbiol 50: 127–134. [CrossRef] [PubMed]
    [Google Scholar]
  10. Kelly K. L. . ( 1964;). Inter-Society Color Council – National Bureau of Standards Color-Name Charts Illustrated with Centroid Colors. Washington, DC:: US Government Printing Office;.
    [Google Scholar]
  11. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. et al. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62: 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  12. Kirby B. M. , Meyers P. R. . ( 2010;). Micromonospora tulbaghiae sp. nov., isolated from the leaves of wild garlic, Tulbaghia violacea . . Int J Syst Evol Microbiol 60: 1328–1333. [CrossRef]
    [Google Scholar]
  13. Koch C. , Kroppenstedt R. M. , Stackebrandt E. . ( 1996;). Intrageneric relationships of the actinomycete genus Micromonospora . . Int J Syst Bacteriol 46: 383–387. [CrossRef]
    [Google Scholar]
  14. Lane D. J. . ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniquesin Bacterial Systematics , pp. 115–148. Edited by Stackebrandt E. . ChichesterGoodfellow:: Wiley;.
    [Google Scholar]
  15. Minnikin D. E. , O'Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2: 233–241. [CrossRef]
    [Google Scholar]
  16. Parte A. C. . ( 2014;). LPSN-list of prokaryotic names with standing in nomenclature. . Nucleic Acids Res 42: 613–616. [CrossRef]
    [Google Scholar]
  17. Phongsopitanun W. , Kudo T. , Mori M. , Shiomi K. , Pittayakhajonwut P. , Suwanborirux K. , Tanasupawat S. . ( 2015;). Micromonospora fluostatini sp. nov., isolated from marine sediment. . Int J Syst Evol Microbiol 65: 4417–4423. [CrossRef] [PubMed]
    [Google Scholar]
  18. Saitou N. , Nei M. . ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4: 406–425.[PubMed]
    [Google Scholar]
  19. Sasser M. . ( 1990;). Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids. . MIDI Technical Note 101. Newark, DE:: MIDI Inc;.
    [Google Scholar]
  20. Shirling E. B. , Gottlieb D. . ( 1966;). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16: 313–340. [CrossRef]
    [Google Scholar]
  21. Staneck J. L. , Roberts G. D. . ( 1974;). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28: 226–231.[PubMed]
    [Google Scholar]
  22. Supong K. , Suriyachadkun C. , Tanasupawat S. , Suwanborirux K. , Pittayakhajonwut P. , Kudo T. , Thawai C. . ( 2013a;). Micromonospora sediminicola sp. nov., isolated from marine sediment. . Int J Syst Evol Microbiol 63: 570–575. [CrossRef] [PubMed]
    [Google Scholar]
  23. Supong K. , Suriyachankun C. , Pittayakhajonwut P. , Suwanborirux K. , Thawai C. . ( 2013b;). Micromonospora spongicola sp. nov., an actinomycete isolated from a marine sponge in the gulf of Thailand. . J Antibiot 66: 505–509.
    [Google Scholar]
  24. Suriyachadkun C. , Chunhametha S. , Thawai C. , Tamura T. , Potacharoen W. , Kirtikara K. , Sanglier J. J. . ( 2009;). Planotetraspora thailandica sp. nov., isolated from soil in Thailand. . Int J Syst Evol Microbiol 59: 992–997. [CrossRef] [PubMed]
    [Google Scholar]
  25. Tamaoka J. , Komagata K. . ( 1984;). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25: 125–128. [CrossRef]
    [Google Scholar]
  26. Tamaoka J. . ( 1994;). Determination of DNA base compositon. . In Chemical Methods in Prokaryotic Systematics , pp. 463–470. Edited by Goodfellow M. , O’Donnell A. G. . Chichester:: Wiley;.
    [Google Scholar]
  27. Tamura K. , Stecher G. , Peterson D. , Filipski A. , Kumar S. . ( 2013;). mega6: Molecular Evolutionary Genetics Analysis version 6.0. . Mol Biol Evol 30: 2725–2729. [CrossRef] [PubMed]
    [Google Scholar]
  28. Thawai C. . ( 2015;). Micromonospora costi sp. nov., isolated from a leaf of Costus speciosus. . Int J Syst Evol Microbiol 65: 1456–1461. [CrossRef] [PubMed]
    [Google Scholar]
  29. Thawai C. , Kittiwongwattana C. , Thanaboripat D. , Laosinwattana C. , Koohakan P. , Parinthawong N. . ( 2015;). Micromonospora oryzae sp. nov., isolated from roots of upland rice. . Int J Syst Evol Microbiol 65: 3818–3823. [CrossRef]
    [Google Scholar]
  30. Thawai C. , Tanasupawat S. , Itoh T. , Suwanborirux K. , Suzuki K. , Kudo T. . ( 2005;). Micromonospora eburnea sp. nov., isolated from a Thai peat swamp forest. . Int J Syst Evol Microbiol 55: 417–422. [CrossRef] [PubMed]
    [Google Scholar]
  31. Thompson J. D. , Higgins D. G. , Gibson T. J. . ( 1994;). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 11: 4673–4680. [CrossRef]
    [Google Scholar]
  32. Tomiyasu I. . ( 1982;). Mycolic acid composition and thermally adaptative changes in Nocardia asteroides . . J Bacteriol 151: 828–837.[PubMed]
    [Google Scholar]
  33. Uchida K. , Aida K. . ( 1984;). An improved method for the glycolate test for simple identification of the acyl type of bacterial cell walls. . J Gen Appl Microbiol 30: 131–134. [CrossRef]
    [Google Scholar]
  34. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. et al. ( 1987;). International committee on Systematic Bacteriology. Report of the ad hoc committee on the reconciliation of approaches to bacterial systematic. . Int J Syst Bacteriol 37: 463–464.[CrossRef]
    [Google Scholar]
  35. Xiang W. , Yu C. , Liu C. , Zhao J. , Yang L. , Xie B. , Li L. , Hong K. , Wang X. et al. ( 2014;). Micromonospora polyrhachis sp. nov., an actinomycete isolated from edible Chinese black ant (Polyrhachis vicina Roger). . Int J Syst Evol Microbiol 64: 495–500. [CrossRef] [PubMed]
    [Google Scholar]
  36. Zhao J. , Guo L. , Liu C. , Zhang Y. , Guan X. , Li J. , Xu S. , Xiang W. , Wang X. , Zhang Y. . ( 2016;). Micromonospora lycii sp. nov., a novel endophytic actinomycete isolated from wolfberry root (Lycium chinense Mill). . J Antibiot 69: 153–158. [CrossRef] [PubMed]
    [Google Scholar]
  37. Ørskov J. . ( 1923;). Investigations Into the Morphology of the Ray Fungi. Copenhagen:: Levin and Munksgaard;.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001175
Loading
/content/journal/ijsem/10.1099/ijsem.0.001175
Loading

Data & Media loading...

Supplements

Supplementary File 1



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error