1887

Abstract

The novel actinomycete, strain CH3-3, was isolated from mangrove sediment collected from Chonburi Province, Thailand. On the basis of its spore morphology and chemotaxonomic characteristics, the strain belonged to the genus . It contained -diaminopimelic acid, glucose, mannose, xylose, ribose and rhamnose in the whole-cell hydrolysate, MK-10(H), MK-10(H) and MK-10(H) as major menaquinones, and iso-C, iso-C and iso-C as major cellular fatty acids. analysis of 16S rRNA gene sequences revealed that the strain showed highest similarity with NEAU-CX1(98.97 %) and 2–30-b(28) (98.97 %). Based on phylogenetic tree analysis of the 16S rRNA gene sequence, the strain formed a cluster with NEAU-CX1, DSM 43171, 2-30-b(28) and DSM 43821. On the basis of phenotypic differences and DNA–DNA relatedness evidence, strain CH3-3 could be clearly distinguished from the closely related species of the genus and represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is CH3-3 (=JCM 18523=PCU 350=TISTR 2396).

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2016-08-01
2020-09-20
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References

  1. Arai T.. 1975; Culture Media for Actinomycetes Japan, Tokyo: The Society for Actinomycetes;
    [Google Scholar]
  2. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E.. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol100:221–230 [CrossRef][PubMed]
    [Google Scholar]
  3. Fang B., Liu C., Guan X., Song J., Zhao J., Liu H., Li C., Ning W., Wang X., Xiang W.. 2015; Two new species of the genus Micromonospora: Micromonospora palomenae sp. nov. and Micromonospora harpali sp. nov. isolated from the insects. Antonie Van Leeuwenhoek108:141–150 [CrossRef][PubMed]
    [Google Scholar]
  4. Felsenstein J.. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol17:368–376[PubMed][CrossRef]
    [Google Scholar]
  5. Felsenstein J.. 1983; Parsimony in systematics: biological and statistical issues. Annu Rev Ecol Syst14:313–333 [CrossRef]
    [Google Scholar]
  6. Felsenstein J.. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution39:783–791 [CrossRef]
    [Google Scholar]
  7. Garcia L. C., Martínez-Molina E., Trujillo M. E.. 2010; Micromonospora pisi sp. nov., isolated from root nodules of Pisum sativum . Int J Syst Evol Microbiol60:331–337 [CrossRef][PubMed]
    [Google Scholar]
  8. Genilloud O.. 2012; Genus I. Micromonospora Ørskov 1923, 156AL. In Bergey’s Manual of Systematic Bacteriology, 2nd edn.Vol 5 , pp.1039–1056 Edited by Goodfellow M., Kämpfer P., Busse M.-J., Trujillo M. E., Suzuki K., Ludwig W., Whitman W. B.. NewYork: Springer;
    [Google Scholar]
  9. Kasai H., Tamura T., Harayama S.. 2000; Intrageneric relationships among Micromonospora species deduced from gyrB-based phylogeny and DNA relatedness. Int J Syst Evol Microbiol50:127–134 [CrossRef][PubMed]
    [Google Scholar]
  10. Kelly K. L.. 1964; Inter-Society Color Council – National Bureau of Standards Color-Name Charts Illustrated with Centroid Colors Washington, DC: US Government Printing Office;
    [Google Scholar]
  11. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  12. Kirby B. M., Meyers P. R.. 2010; Micromonospora tulbaghiae sp. nov., isolated from the leaves of wild garlic, Tulbaghia violacea . Int J Syst Evol Microbiol60:1328–1333 [CrossRef]
    [Google Scholar]
  13. Koch C., Kroppenstedt R. M., Stackebrandt E.. 1996; Intrageneric relationships of the actinomycete genus Micromonospora . Int J Syst Bacteriol46:383–387 [CrossRef]
    [Google Scholar]
  14. Lane D. J.. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniquesin Bacterial Systematics , pp.115–148 Edited by Stackebrandt E.. ChichesterGoodfellow: Wiley;
    [Google Scholar]
  15. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods2:233–241 [CrossRef]
    [Google Scholar]
  16. Parte A. C.. 2014; LPSN-list of prokaryotic names with standing in nomenclature. Nucleic Acids Res42:613–616 [CrossRef]
    [Google Scholar]
  17. Phongsopitanun W., Kudo T., Mori M., Shiomi K., Pittayakhajonwut P., Suwanborirux K., Tanasupawat S.. 2015; Micromonospora fluostatini sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol65:4417–4423 [CrossRef][PubMed]
    [Google Scholar]
  18. Saitou N., Nei M.. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol4:406–425[PubMed]
    [Google Scholar]
  19. Sasser M.. 1990; Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids. MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  20. Shirling E. B., Gottlieb D.. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol16:313–340[CrossRef]
    [Google Scholar]
  21. Staneck J. L., Roberts G. D.. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol28:226–231[PubMed]
    [Google Scholar]
  22. Supong K., Suriyachadkun C., Tanasupawat S., Suwanborirux K., Pittayakhajonwut P., Kudo T., Thawai C.. 2013a; Micromonospora sediminicola sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol63:570–575 [CrossRef][PubMed]
    [Google Scholar]
  23. Supong K., Suriyachankun C., Pittayakhajonwut P., Suwanborirux K., Thawai C.. 2013b; Micromonospora spongicola sp. nov., an actinomycete isolated from a marine sponge in the gulf of Thailand. J Antibiot66:505–509
    [Google Scholar]
  24. Suriyachadkun C., Chunhametha S., Thawai C., Tamura T., Potacharoen W., Kirtikara K., Sanglier J. J.. 2009; Planotetraspora thailandica sp. nov., isolated from soil in Thailand. Int J Syst Evol Microbiol59:992–997 [CrossRef][PubMed]
    [Google Scholar]
  25. Tamaoka J., Komagata K.. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett25:125–128 [CrossRef]
    [Google Scholar]
  26. Tamaoka J.. 1994; Determination of DNA base compositon. In Chemical Methods in Prokaryotic Systematics , pp.463–470 Edited by Goodfellow M., O’Donnell A. G.. Chichester: Wiley;
    [Google Scholar]
  27. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. 2013; mega6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  28. Thawai C.. 2015; Micromonospora costi sp. nov., isolated from a leaf of Costus speciosus. Int J Syst Evol Microbiol65:1456–1461 [CrossRef][PubMed]
    [Google Scholar]
  29. Thawai C., Kittiwongwattana C., Thanaboripat D., Laosinwattana C., Koohakan P., Parinthawong N.. 2015; Micromonospora oryzae sp. nov., isolated from roots of upland rice. Int J Syst Evol Microbiol65:3818–3823 [CrossRef]
    [Google Scholar]
  30. Thawai C., Tanasupawat S., Itoh T., Suwanborirux K., Suzuki K., Kudo T.. 2005; Micromonospora eburnea sp. nov., isolated from a Thai peat swamp forest. Int J Syst Evol Microbiol55:417–422 [CrossRef][PubMed]
    [Google Scholar]
  31. Thompson J. D., Higgins D. G., Gibson T. J.. 1994; CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res11:4673–4680 [CrossRef]
    [Google Scholar]
  32. Tomiyasu I.. 1982; Mycolic acid composition and thermally adaptative changes in Nocardia asteroides . J Bacteriol151:828–837[PubMed]
    [Google Scholar]
  33. Uchida K., Aida K.. 1984; An improved method for the glycolate test for simple identification of the acyl type of bacterial cell walls. J Gen Appl Microbiol30:131–134 [CrossRef]
    [Google Scholar]
  34. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. 1987; International committee on Systematic Bacteriology. Report of the ad hoc committee on the reconciliation of approaches to bacterial systematic. Int J Syst Bacteriol 37:463–464[CrossRef]
    [Google Scholar]
  35. Xiang W., Yu C., Liu C., Zhao J., Yang L., Xie B., Li L., Hong K., Wang X. et al. 2014; Micromonospora polyrhachis sp. nov., an actinomycete isolated from edible Chinese black ant (Polyrhachis vicina Roger). Int J Syst Evol Microbiol64:495–500 [CrossRef][PubMed]
    [Google Scholar]
  36. Zhao J., Guo L., Liu C., Zhang Y., Guan X., Li J., Xu S., Xiang W., Wang X., Zhang Y.. 2016; Micromonospora lycii sp. nov., a novel endophytic actinomycete isolated from wolfberry root (Lycium chinense Mill). J Antibiot69:153–158 [CrossRef][PubMed]
    [Google Scholar]
  37. Ørskov J.. 1923; Investigations Into the Morphology of the Ray Fungi Copenhagen: Levin and Munksgaard;
    [Google Scholar]
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