gen. nov., sp. nov., isolated from seawater and reclassification of as comb. nov. Free

Abstract

A Gram-stain-negative, non-endospore-forming, strictly aerobic, irregular rod-shaped bacterium without flagellum, designated strain H94, was isolated by the high-throughput cultivation method from seawater of an amphioxus breeding zone in the coastal region of Qingdao, China. Growth was observed at 4–37 °C (optimum 28 °C), at pH 6.0–10.0 (optimum pH 7.0) and in the presence of 1–12 % (w/v) NaCl (optimum 1–2 %). The predominant cellular fatty acids were C 9, C and C 9. The polar lipids comprised phosphatidylethanolamine, phosphatidylglycerol and an unidentified phosphoglycolipid. The major respiratory quinone was ubiquinone-9 (Q-9). The genomic DNA G+C content of strain H94 was 56.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain H94 shared the highest similarity (95.9 %) with NBRC 102683, and exhibited 92.9 % and 92.1 % similarity with the two other recognized species, KCTC 2396 and DSM 17046, respectively. The phylogenetic position revealed that strain H94 formed a stable distinct lineage cluster together with NBRC 102683 and this result was further confirmed by multilocus sequence analysis based on housekeeping genes and . On the basis of the polyphasic taxonomic analyses, strain H94 is considered to represent a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is H94 (=CGMCC 1.10800=JCM 17555). It is also proposed that should be reclassified within the genus as comb. nov. (type strain NBRC 102683=IMCC 3113=KCCM 42675). The type species of the genus is comb. nov.

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2016-08-01
2024-03-29
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