1887

Abstract

A Gram-stain-negative, motile by gliding, non-spore-forming and oval-shaped bacterial strain designated T1-10 was isolated from pine forest soil, and its taxonomic position was investigated using a polyphasic approach. Growth occurred at 10–37 °C (optimum, 30 °C), at pH 6–7 and in the presence of 0–1 % (w/v) (optimum, 0 %) NaCl. Flexirubin-type pigments were produced. On the basis of 16S rRNA gene sequence similarity, strain T1-10 was assigned to the genus of the phylum , and the most closely related species was THG-DT86 with 97.11 % sequence similarity, but the strain formed an independent lineage in the phylogenetic tree. The genomic DNA G+C content of strain T1-10 was 42.5 mol%. The main cellular fatty acids were iso-C G, iso-C and iso-C 3-OH. The only isoprenoid quinone detected in the strain was MK-7, and the major polyamine was homospermidine. The major polar lipids were phosphatidylethanolamine and unidentified aminophospholipids. Strain T1-10 could be distinguished from related species by physiological and biochemical properties. Phenotypic and phylogenetic data supported that strain T1-10 represents a novel species of the genus , for which the name sp. nov. is proposed (type strain T1-10=KCTC 42277=JCM 31014).

Keyword(s): gliding , Pinus , soil and Taibaiella
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2016-08-01
2021-07-26
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References

  1. Busse H.-J., Bunka S., Hensel A., Lubitz W. 1997; Discrimination of members of the family Pasteurellaceae based on polyamine patterns. Int J Syst Evol Microbiol 47:698–708 [View Article]
    [Google Scholar]
  2. Fautz E., Reichenbach H. 1980; A simple test for flexirubin-type pigments. FEMS Microbiol Lett 8:87–91 [View Article]
    [Google Scholar]
  3. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376[PubMed] [CrossRef]
    [Google Scholar]
  4. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783 [View Article]
    [Google Scholar]
  5. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
    [Google Scholar]
  6. Gonzalez J. M., Saiz-Jimenez C. 2002; A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4:770–773[PubMed] [CrossRef]
    [Google Scholar]
  7. Jeon Y. S., Lee K., Park S. C., Kim B. S., Cho Y. J., Ha S. M., Chun J. 2014; EzEditor: a versatile sequence alignment editor for both rRNA- and protein-coding genes. Int J Syst Evol Microbiol 64:689–691 [View Article][PubMed]
    [Google Scholar]
  8. Jukes T. H., Cantor C. R. 1969 Evolution of protein molecules In Mammalian Protein Metabolism vol. 3 pp. 21–132 Edited by Munro H. N. New York: Academic Press; [CrossRef]
    [Google Scholar]
  9. Kim M. K., Kim T. W., Kim T. S., Joung Y., Han J. H., Kim S. B. 2016; Fluviicoccus keumensis gen. nov., sp. nov., isolated from freshwater. Int J Syst Evol Microbiol 66:201–205 [View Article][PubMed]
    [Google Scholar]
  10. Kim M., Oh H. S., Park S. C., Chun J. 2014; Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 64:346–351 [View Article][PubMed]
    [Google Scholar]
  11. Kovacs N. 1956; Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178:703 [View Article][PubMed]
    [Google Scholar]
  12. Minnikin D., Patel P., Alshamaony L., Goodfellow M. 1977; Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Evol Bacteriol 27:104–117 [View Article]
    [Google Scholar]
  13. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  14. Sasser M. 1990; Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids. MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  15. Scherer P., Kneifel H. 1983; Distribution of polyamines in methanogenic bacteria. J Bacteriol 154:1315–1322[PubMed]
    [Google Scholar]
  16. Singh H., Du J., Won K., Yang J. E., Akter S., Kim K. Y., Yin C., Yi T. H. 2015; Taibaiella yonginensis sp. nov., a bacterium isolated from soil of Yongin city. Antonie van Leeuwenhoek 108:517–524 [View Article][PubMed]
    [Google Scholar]
  17. Son H. M., Kook M., Kim J. H., Yi T. H. 2014; Taibaiella koreensis sp. nov., isolated from soil of a ginseng field. Int J Syst Evol Microbiol 64:1018–1023 [View Article][PubMed]
    [Google Scholar]
  18. Stackebrandt E., Ebers J. 2006; Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33:152–155
    [Google Scholar]
  19. Szabó I., Szoboszlay S., Táncsics A., Szerdahelyi S. G., Szu˝cs Á., Radó J., Benedek T., Szabó L., Daood H. G. et al. 2016; Taibaiella coffeisoli sp. nov., isolated from the soil of a coffee plantation. Int J Syst Evol Microbiol 66:1627–1632 [View Article][PubMed]
    [Google Scholar]
  20. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013; mega6: Molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  21. Tan X., Zhang R. G., Meng T. Y., Liang H. Z., Lv J. 2014; Taibaiella chishuiensis sp. nov., isolated from freshwater. Int J Syst Evol Microbiol 64:1795–1801 [View Article][PubMed]
    [Google Scholar]
  22. Zhang L., Wang Y., Wei L., Wang Y., Shen X., Li S. 2013; Taibaiella smilacinae gen. nov., sp. nov., an endophytic member of the family Chitinophagaceae isolated from the stem of Smilacina japonica, and emended description of Flavihumibacter petaseus . Int J Syst Evol Microbiol 63:3769–3776 [View Article][PubMed]
    [Google Scholar]
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