1887

Abstract

A Gram-stain-negative, strictly aerobic, cream-coloured, motile, chemoheterotrophic, rod-shaped bacterium, designated strain KMU-32, was isolated from seawater at Najeong Beach in Korea. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the novel isolate was affiliated with the family Rhodobacteraceae of the class Alphaproteobacteria and that it showed highest sequence similarity (98.2 %) to Cribrihabitans neustonicus CC-AMHB-3. The DNA–DNA relatedness value between strain KMU-32 and C. neustonicus CC-AMHB-3was 48.5 ± 4 %. The DNA G+C content of strain KMU-32 was determined to be 63.7 mol%. Ubiquinone 10 (Q-10) was the sole respiratory quinone. The predominant cellular fatty acid was C18 : 1 ω7c. Strain KMU-32 had diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unidentified aminolipid and an unidentified lipid as polar lipids. From the distinct phylogenetic position and combination of genotypic and phenotypic characteristics, strain KMU-32 is considered to represent a novel species of the genus Cribrihabitans , for which the name Cribrihabitans pelagius sp. nov. is proposed. The type strain is KMU-32 (= KCTC 42981 = NBRC 111834).

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2016-08-01
2019-12-11
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References

  1. Bernardet J. F. , Nakagawa Y. , Holmes B. . ( 2002;) Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52: 1049–1070. [CrossRef] [PubMed]
    [Google Scholar]
  2. Chen Z. , Liu Y. , Liu L. Z. , Zhong Z. P. , Liu Z. P. , Liu Y. . ( 2014;). Cribrihabitans marinus gen. nov., sp. nov., isolated from a biological filter in a marine recirculating aquaculture system. . Int J Syst Evol Microbiol 64: 1257–1263. [CrossRef] [PubMed]
    [Google Scholar]
  3. Collins M. D. , Jones D. . ( 1981;). A note on the separation of natural mixtures of bacterial ubiquinones using reverse-phase partition thin-layer chromatography and high performance liquid chromatography. . J Appl Bacteriol 51: 129–134.[PubMed] [CrossRef]
    [Google Scholar]
  4. Collins C. H. , Lyne P. M. . ( 1984;). Microbiological Methods, , 5th edn., London:: Butterworth;.
    [Google Scholar]
  5. Dittmer J. C. , Lester R. L. . ( 1964;). A simple, specific spray for the detection of phospholipids on thin-layer chromoatograms. . J Lipid Res 15: 126–127.
    [Google Scholar]
  6. Felsenstein J. . ( 1985;). Confidence limits on phylogenies: An approach using the bootstrap. . Evolution 39: 783–791.[CrossRef]
    [Google Scholar]
  7. Fitch W. M. . ( 1971;). Towards defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20: 406–416.[CrossRef]
    [Google Scholar]
  8. Garrity G. M. , Bell J. A. , Lilburn T. . ( 2005;). Family I. Rhodobacteraceae fam. nov. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn.,vol. 2C pp. Brenner D. J. , Krieg N. R. , Staley J. T. , Garrity G. M. . New York:: Springer;.
    [Google Scholar]
  9. Hameed A. , Shahina M. , Lin S. Y. , Lai W. A. , Liu Y. C. , Hsu Y. H. , Young C. C. . ( 2014;). Cribrihabitans neustonicus sp. nov., isolated from coastal surface seawater, and emended description of the genus Cribrihabitans Chen et al. 2014. . Int J Syst Evol Microbiol 64: 3897–3903. [CrossRef] [PubMed]
    [Google Scholar]
  10. Hansen G. H. , Sørheim R. . ( 1991;). Improved method for phenotypical characterization of marine bacteria. . J Microbiol Methods 13: 231–241.[CrossRef]
    [Google Scholar]
  11. Iwaki H. , Yasukawa N. , Fujioka M. , Takada K. , Hasegawa Y. . ( 2013;). Isolation and characterization of a marine cyclohexylacetate-degrading bacterium Lutimaribacter litoralis sp. nov., and reclassification of Oceanicola pacificus as Lutimaribacter pacificus comb. nov. . Curr Microbiol 66: 588–593. [CrossRef] [PubMed]
    [Google Scholar]
  12. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. et al. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62: 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  13. Kimura M. . ( 1983;). The Neutral Theory of Molecular Evolution, Cambridge:: Cambridge University Press;.[CrossRef]
    [Google Scholar]
  14. Komagata K. , Suzuki K. . ( 1987;). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19: 161–207.[CrossRef]
    [Google Scholar]
  15. Marmur J. . ( 1961;). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. . J Mol Biol 3: 208–218.[CrossRef]
    [Google Scholar]
  16. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989;). Precise measurement Of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39: 159–167.[CrossRef]
    [Google Scholar]
  17. Minnikin D. E. , O’Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinines and polar lipids. . J Microbiol Meth 2: 233–241.[CrossRef]
    [Google Scholar]
  18. Park S. , Park D. S. , Bae K. S. , Yoon J. H. . ( 2014;). Phaeobacter aquaemixtae sp. nov., isolated from the junction between the ocean and a freshwater spring. . Int J Syst Evol Microbiol 64: 1378–1383. [CrossRef] [PubMed]
    [Google Scholar]
  19. Saitou N. , Nei M. . ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4: 406–425.[PubMed]
    [Google Scholar]
  20. Sasser M. . ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids. . MIDI Technical Note 101, Newark, DE:: MIDI Inc;.
    [Google Scholar]
  21. Stackebrandt E. , Goebel B. M. . ( 1994;). Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44: 846–849.[CrossRef]
    [Google Scholar]
  22. Suzuki K. , Kaneko T. , Komagata K. . ( 1981;). Deoxyribonucleic acid homologies among coryneform bacteria. . Int J Syst Bacteriol 31: 131–138.[CrossRef]
    [Google Scholar]
  23. Tamura K. , Peterson D. , Petersen N. , Stecher G. , Nei M. , Kumar S. . ( 2011;). MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28: 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  24. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997;). The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25: 4876–4882.[PubMed] [CrossRef]
    [Google Scholar]
  25. Wang L. , Liu Y. , Shi X. , Wang Y. , Zheng Y. , Dai X. , Zhang X. H. . ( 2016;). Xuhuaishuia manganoxidans gen. nov., sp. nov., a manganese-oxidizing bacterium isolated from deep-sea sediments from the Pacific Polymetallic Nodule Province. . Int J Syst Evol Microbiol 66: 1521–1526. [CrossRef] [PubMed]
    [Google Scholar]
  26. Weisburg W. G. , Barns S. M. , Pelletier D. A. , Lane D. J. . ( 1991;). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173: 697–703.[PubMed]
    [Google Scholar]
  27. Worliczek H. L. , Kämpfer P. , Rosengarten R. , Tindall B. J. , Busse H. J. . ( 2007;). Polar lipid and fatty acid profiles--re-vitalizing old approaches as a modern tool for the classification of mycoplasmas?. Syst Appl Microbiol 30: 355–370. [CrossRef] [PubMed]
    [Google Scholar]
  28. Zhang G. , Yang Y. , Wang S. , Sun Z. , Jiao K. . ( 2015;). Alkalimicrobium pacificum gen. nov., sp. nov., a marine bacterium in the family Rhodobacteraceae . . Int J Syst Evol Microbiol 65: 2453–2458. [CrossRef] [PubMed]
    [Google Scholar]
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