1887

Abstract

A novel bacterial strain, designated as LNUB461, was isolated from soil sample taken from the countryside of Shenyang, Liaoning Province, China. The isolate was a Gram-stain-positive, aerobiotic, motile, endospore-forming and rod-shaped bacterium. The organism grew optimally at 30–33 °C, pH 6.5–7.0 and in the absence of NaCl. Phylogenetic analysis based on the nearly full-length 16S rRNA gene sequence revealed high sequence similarity with XJ259 (98.5 %), B538 (96.8 %), DS-1 (96.1 %) and RLAHU15 (96.1 %). The predominant cellular fatty acid and the only menaquinone were anteiso-C and MK-7, respectively. The main polar lipids of LNUB461 included phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylcholine (PC) and two unknown amino phospholipids (APL), and the cell-wall peptidoglycan was -diaminopimelic acid (A1γ). The DNA G+C content of LNUB461 was 49.1 mol%. The DNA–DNA hybridization value between LNUB461 and the most closely related species () was 41.8 %. On the basis of these data, LNUB461 was classified as representing a novel species of the genus , for which the name sp. nov was proposed. The type strain is LNUB461 (=JCM 30712=CGMCC 1.15101).

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2016-08-01
2020-09-25
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