RT Journal Article SR Electronic(1) A1 Cha, In-Tae A1 Park, Inhye A1 Lee, Hae-Won A1 Lee, Hyunjin A1 Park, Jung-Min A1 Roh, Seong Woon A1 Choi, Hak-Jong A1 Nam, Young-Do A1 Lee, Yoo Kyung A1 Seo, Myung-JiYR 2016 T1 Pseudoruegeria aestuarii sp. nov., of the family Rhodobacteraceae, isolated from a tidal flat JF International Journal of Systematic and Evolutionary Microbiology, VO 66 IS 8 SP 3125 OP 3131 DO https://doi.org/10.1099/ijsem.0.001156 PB Microbiology Society, SN 1466-5034, AB A Gram-stain-negative, non-motile, aerobic and rod-shaped bacterium, designated strain MME-001T, was isolated from the tidal flat of Muui-do in the Republic of Korea. Phylogenetic trees based on the 16S rRNA gene sequence showed that strain MME-001T belonged to the genus Pseudoruegeria in the family Rhodobacteraceae and that it shared the highest 16S rRNA gene sequence similarity with Pseudoruegeria sabulilitoris GJMS-35T (98.0 % similarity of the 16S rRNA gene). Growth of strain MME-001T occurred in the presence of 1.0–7.0 % (w/v) NaCl at 15−40 °C and pH 7.0–9.0, with optimal growth in the presence of 2.0–3.0 % (w/v) NaCl at 25–30 °C and pH 7.0. Ubiquinone-10 was the major respiratory quinone. Major polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and an unidentified lipid. The major cellular fatty acids were C18 : 1 ω7c and C16 : 0. The genomic DNA G+C content was 62 mol%. DNA-DNA hybridization values between strain MME-001T and P. sabulilitoris KCTC 42111T, ‘ Pseudoruegeria limi ’ KCTC 32460, Pseudoruegeria lutimaris KCTC 22690T, Pseudoruegeria aquimaris KCTC 12737T and Pseudoruegeriahaliotis KACC 17214T was 36±5, 57±7, 34±4, 18±5 and 21±3 %, respectively. Based on the phenotypic and phylogenetic taxonomical properties, this strain MME-001T represents a novel species of the genus Pseudoruegeria , for which the name Pseudoruegeria aestuarii sp. nov. is proposed. The type strain is MME-001T (=KCCM 43133T=JCM 30751T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001156