A Gram-stain-positive, coccoid, oxidase-negative, non-motile isolate from exhaust air of a pig barn, collected on 17 September 2014 and designated strain 140917-MRSA-09T, was subjected to a comprehensive taxonomic investigation. A comparative analysis of the 16S rRNA gene sequence showed highest similarities to Rothia amarae, Rothia terrae and Rothia endophytica (all <97.8 %). The G+C content of the genomic DNA was 58.9 mol %. The quinone system consisted of the major menaquinones MK-8 and MK-7. The polar lipid profile of strain 140917-MRSA-09T contained the major lipids diphosphatidylglycerol and phosphatidylglycerol and moderate amounts of dimannosylglyceride and trimannosyldiacylglycerol. The polyamine pattern was composed of the major amines putrescine and spermidine. In the fatty acid profile, iso- and anteiso-branched acids predominated (anteiso-C15 : 0, anteiso-C17 : 0, iso-C16 : 0). The strain showed a chemoheterotrophic metabolism and was able to grow aerobically well on nutrient-rich media at temperatures from 15–36 °C (weak at 42 °C), pH 5.5–9.5 and NaCl concentrations ranging from 0 to 7 % (w/v). Growth under anaerobic conditions was weak. Physiological traits as well as unique traits in the quinone pattern and the fatty acid pattern distinguished strain 140917-MRSA-09T from the most closely related species. All these data showed that strain 140917-MRSA-09T is a representative of a novel species of the genus Rothia, for which we propose the name Rothia aerolata sp. nov. The type strain is 140917-MRSA-09T (=LMG 29446T=CCM 8669T).
AltenburgeraP.,
KämpferbP.,
MakristathiscA.,
LubitzaW.,
BusseaH. J.1996; Classification of bacteria isolated from a medieval wall painting. Journal of Biotechnology 47:39–52[CrossRef]
AltenburgerP.,
KampferP.,
AkimovV. N.,
LubitW.,
BusseH.-J.1997; Polyamine Distribution in Actinomycetes with Group B Peptidoglycan and Species of the Genera Brevibacterium, Corynebacterium, and Tsukamurella
. Int J Syst Bacteriol 47:270–277[CrossRef]
CollinsM. D.,
HutsonR. A.,
BåverudV.,
FalsenE.2000; Characterization of a Rothia-like organism from a mouse: description of Rothia nasimurium sp. nov. and reclassification of Stomatococcus mucilaginosus as Rothia mucilaginosa comb. nov. Int J Syst Evol Microbiol 50:1247–1251 [View Article][PubMed]
EmbleyT. M.,
GoodfellowM.,
MinnikinD. E.,
O'DonnellA. G.1984; Lipid and wall amino acid composition in the classification of Rothia dentocariosa
. Zentralbl Bakteriol Mikrobiol Hyg A 257:285–295[PubMed]
FelsensteinJ.2005; PHYLIP (Phylogeny Inference Package) version 3.6. Distributed by the author. Department of Genome Sciences Seattle: University of Washington;
GerhardtP.,
MurrayR. G. E.,
WoodW. A.,
KriegN. R.
(editors) 1994Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
GonzalezJ. M.,
Saiz-JimenezC.2002; A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4:770–773[PubMed][CrossRef]
GärtnerA.,
GessnerA.,
GromöllerS.,
KlugK.,
KnustS.,
JäckelU.2016; Emissionen aus Schweinemastanlagen – Untersuchungen zur Zusammensetzung der Bakteriengemeinschaft und Antibiotikaresistenz. Gefahrstoffe- Reinhaltung Der Luft/Air Quality Control 1:31–38
JukesT. H.,
CantorC. R.1969; Evolution of the protein molecules. In Mammalian Protein Metabolism pp. 21–132 Edited by
MunroH. N.
New York: Academic Press; [CrossRef]
KämpferP.,
SteiofM.,
DottW.1991; Microbiological characterization of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 21:227–251 [View Article][PubMed]
KämpferP.,
KroppenstedtR. M.1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005[CrossRef]
LiuZ. X.,
YangL. L.,
HuangY.,
ZhaoH.,
LiuH.,
TangS. K.,
LiW. J.,
ChenY. G.2013; Rothia marina sp. nov., isolated from an intertidal sediment of the South China Sea. Antonie van Leeuwenhoek 104:331–337 [View Article][PubMed]
SchaalK. P.1992; The genera Actinomyces, Arcanobacterium, and Rothia
. In The Prokaryotes, 2nd Edn pp. 850–905 Edited by
BalowsA. ,
TruäperH. G. ,
DworkinM. ,
HarderW. ,
SchleiferK.-H. .
New York: Springer;
StackebrandtE.,
RaineyF. A.,
Ward-raineyN. L.1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491[CrossRef]
StamatakisA.2006; RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22:2688–2690 [View Article][PubMed]
TindallB. J.1990b; A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130[CrossRef]
YarzaP.,
RichterM.,
PepliesJ.,
EuzebyJ.,
AmannR.,
SchleiferK. H.,
LudwigW.,
GlöcknerF. O.,
Rosselló-MóraR.2008; The All-Species Living Tree project: a 16S rRNA-based phylogenetic tree of all sequenced type strains. Syst Appl Microbiol 31:241–250 [View Article][PubMed]