1887

Abstract

A Gram-stain-negative, aerobic and slightly halophilic bacterium was isolated from the South China Sea, and was subjected to characterization using a polyphasic taxonomic approach. Cells of the isolate, designated NH83, were non-motile and rod-shaped. On the basis of 16S rRNA gene sequence analysis, strain NH83was closely related to members of the genera (with sequence similarity of 92.9 %), (92.8 %), (92.6 %), (92.6 %), (91.5–91.9 %), (91.8 %) and (89.6–91.2 %), all of which belong to the family . Phylogenetic analysis indicated that it represented an independent lineage and its closest relatives belonged to the genus . The sole respiratory quinone was MK-6. The major polar lipids were phosphatidylethanolamine, two aminolipids, one aminophospholipid and one unidentified lipid. The principal fatty acids were branched fatty acids, including iso-C, iso-C 3-OH, iso-C, iso-C G and summed feature 3 (iso-C 2-OH and/or Cω7). The genomic DNA G+C content was 41.0 mol%. Strain NH83 was positive for hydrolysis of aesculin, gelatin and Tween 60. Phylogenetic distinctiveness and chemotaxonomic differences, together with differential phenotypic properties, revealed that strain NH83 could be differentiated from closely related genera. Therefore, it is proposed that strain NH83 represents a novel species in a new genus, for which the name gen. nov., sp. nov. (type strain NH83=CGMCC 1.15462=DSM 101478) is proposed.

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2016-08-01
2021-08-01
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References

  1. Bernardet J.-F., Segers P., Vancanneyt M., Berthe F., Kersters K., Vandamme P. 1996; Cutting a gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (Basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46:128–148 [CrossRef]
    [Google Scholar]
  2. Bernardet J. F., Nakagawa Y., Holmes B. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [View Article][PubMed]
    [Google Scholar]
  3. Bernardet J.-F. 2010; Order I. Flavobacteriales ord. nov. In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol. 4105–314 Edited by Krieg N. R., Staley J. T., Brown D. R., Hedlund B. P., Paster B. J., Ward N. L., Ludwig W., Whitman W. B. New York: Springer;
    [Google Scholar]
  4. Dong X.-Z., Cai M.-Y. 2001 Determinative Manual for Routine Bacteriology Beijing: Scientific Press (English translation);
    [Google Scholar]
  5. Farmer J. J. III, Janda J. M., Brenner F. W., Cameron D. N., Birkhead K. M. 2005; Genus I. Vibrio Pacini 1854, 411AL. In Bergey’s Manual of Systematic Bacteriology The Proteobacteria, Part B, the Gammaproteobacteria vol. 2, 2nd edn. pp 494–546 Edited by Brenner D. J., Krieg N. R., Staley J. T. , Garrity G. M. New York: Springer;
    [Google Scholar]
  6. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376[PubMed] [CrossRef]
    [Google Scholar]
  7. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  8. González J. M., Fernández-Gómez B., Fernàndez-Guerra A., Gómez-Consarnau L., Sánchez O., Coll-Lladó M., Del Campo J., Escudero L., Rodríguez-Martínez R. et al. 2008; Genome analysis of the proteorhodopsin-containing marine bacterium Polaribacter sp. MED152 (Flavobacteria). Proc Natl Acad Sci U S A 105:8724–8729 [View Article][PubMed]
    [Google Scholar]
  9. Hildebrand D. C., Palleroni N. J., Hendson M., Toth J., Johnson J. L. 1994; Pseudomonas flavescens sp. nov., isolated from walnut blight cankers. Int J Syst Bacteriol 44:410–415 [View Article][PubMed]
    [Google Scholar]
  10. Jooste, P. J. 1985; The taxonomy and significance of Flavobacterium-Cytophaga strains from dairy sources. PhD thesis University of the Orange Free State;
    [Google Scholar]
  11. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  12. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120[PubMed] [CrossRef]
    [Google Scholar]
  13. Komagata K., Suzuki K. 1987; Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [CrossRef]
    [Google Scholar]
  14. Lami R., Cottrell M. T., Campbell B. J., Kirchman D. L. 2009; Light-dependent growth and proteorhodopsin expression by Flavobacteria and SAR11 in experiments with Delaware coastal waters. Environ Microbiol 11:3201–3209 [View Article][PubMed]
    [Google Scholar]
  15. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184[PubMed]
    [Google Scholar]
  16. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. et al. 2004; ARB: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [View Article][PubMed]
    [Google Scholar]
  17. McBride M. J. 2014; The Family Flavobacteriaceae . In The Prokaryote - Other Major Lineages of Bacteria and the Archaea, 4th edn. pp 643 Edited by Rosenberg E., DeLong E. F., Lory S., Stackebrandt E., Thompson F. New York: Springer;
    [Google Scholar]
  18. Mesbah M., Whitman W. B. 1989; Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. J Chromatogr 479:297–306[PubMed] [CrossRef]
    [Google Scholar]
  19. Park Y. D., Baik K. S., Yi H., Bae K. S., Chun J. 2005; Pseudoalteromonas byunsanensis sp. nov., isolated from tidal flat sediment in Korea. Int J Syst Evol Microbiol 55:2519–2523 [View Article][PubMed]
    [Google Scholar]
  20. Park S., Lee J. S., Lee K. C., Yoon J. H. 2013; Jejudonia soesokkakensis gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from the junction between the ocean and a freshwater spring, and emended description of the genus Aureitalea Park et al. 2012. Antonie Van Leeuwenhoek 104:139–147 [View Article][PubMed]
    [Google Scholar]
  21. Pruesse E., Quast C., Knittel K., Fuchs B. M., Ludwig W., Peplies J., Glöckner F. O. 2007; SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB. Nucleic Acids Res 35:7188–7196 [View Article][PubMed]
    [Google Scholar]
  22. Reichenbach H. 1992; Flavobacteriaceae fam. nov. In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB, List no. 41. Int J Syst Bacteriol 42:327–329 [CrossRef]
    [Google Scholar]
  23. Romanenko L. A., Uchino M., Frolova G. M., Mikhailov V. V. 2007; Marixanthomonas ophiurae gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from a deep-sea brittle star. Int J Syst Evol Microbiol 57:457–462 [View Article][PubMed]
    [Google Scholar]
  24. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  25. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011; MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  26. Thompson J. D., Higgins D. G., Gibson T. J. 1994; CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680[PubMed] [CrossRef]
    [Google Scholar]
  27. Tindall B. J., Sikorski J., Smibert R. M., Kreig N. R. 2007; Phenotypic characterization and the principles of comparative systematics. In Methods for General and Molecular Microbiology, 3rd edn. pp 330–393 Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R. Washington DC, USA: ASM Press;
    [Google Scholar]
  28. Wu Y. H., Xu L., Zhou P., Wang C. S., Oren A., Xu X. W. 2015; Brevirhabdus pacifica gen. nov., sp. nov., isolated from deep-sea sediment in a hydrothermal vent field. Int J Syst Evol Microbiol 65:3645–3651 [View Article][PubMed]
    [Google Scholar]
  29. Xu X. W., Wu Y. H., Zhou Z., Wang C. S., Zhou Y. G., Zhang H. B., Wang Y., Wu M. 2007; Halomonas saccharevitans sp. nov., Halomonas arcis sp. nov. and Halomonas subterranea sp. nov., halophilic bacteria isolated from hypersaline environments of China. Int J Syst Evol Microbiol 57:1619–1624 [View Article][PubMed]
    [Google Scholar]
  30. Yarza P., Richter M., Peplies J., Euzeby J., Amann R., Schleifer K. H., Ludwig W., Glöckner F. O., Rosselló-Móra R. 2008; The All-Species Living Tree project: a 16S rRNA-based phylogenetic tree of all sequenced type strains. Syst Appl Microbiol 31:241–250 [View Article][PubMed]
    [Google Scholar]
  31. Yoshizawa S., Kawanabe A., Ito H., Kandori H., Kogure K. 2012; Diversity and functional analysis of proteorhodopsin in marine Flavobacteria. Environ Microbiol 14:1240–1248 [View Article][PubMed]
    [Google Scholar]
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