1887

Abstract

A Gram-stain-positive, endospore-forming, aerobic and thermophilic bacterium, designated strain LC2-13A, was isolated from Cisolok geyser, West Java, Indonesia, at 50 °C. The isolate was rod-shaped and motile by means of peritrichous flagella. The major cellular fatty acids were iso-C, C and anteiso-C and the major quinone was menaquinone 7. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The genomic DNA G+C content was 56.6 mol% and the major diagnostic diamino acid in the cell-wall peptidoglycan was -diaminopimelic acid. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain LC2-13A is related most closely to DSM 10249(94.86 % similarity), SA-7-6(94.77 %) and SAFN-016 (94.77 %). On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, strain LC2-13A is affiliated to the genus , but could be distinguished from recognized species of this genus. A novel species with the name sp. nov. is thus proposed. The type strain is LC2-13A (=UICC B-42=NRRL B-65368=DSM 101873).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001151
2016-08-01
2019-12-14
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/8/3088.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001151&mimeType=html&fmt=ahah

References

  1. Ash C., Farrow J. A. E., Wallbanks S., Collins M. D.. 1991; Phylogenetic heterogeneity of the genus Bacillus revealed by comparative analysis of small-subunit-ribosomal RNA sequences. Lett Appl Microbiol13:202–206 [CrossRef]
    [Google Scholar]
  2. Ash C., Priest F. G., Collins M. D.. 1993; Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. proposal for the creation of a new genus Paenibacillus . Antonie Van Leeuwenhoek64:253–260 [CrossRef][PubMed]
    [Google Scholar]
  3. Ash C., Priest F. G., Collins M. D.. 1994; Paenibacillus gen. nov. and Paenibacillus polymyxa comb. nov. In validation of the publication of new names and new combinations previously effectively published outside the IJSB, List no. 51. Int J Syst Bacteriol44:852[CrossRef]
    [Google Scholar]
  4. Baik K. S., Choe H. N., Park S. C., Kim E. M., Seong C. N.. 2011; Paenibacillus wooponensis sp. nov., isolated from wetland freshwater. Int J Syst Evol Microbiol61:2763–2768 [CrossRef][PubMed]
    [Google Scholar]
  5. Bouraoui H., Rebib H., Aissa M. B., Touzel J. P., O'donohue M., Manai M.. 2013; Paenibacillus marinum sp. nov., a thermophilic xylanolytic bacterium isolated from a marine hot spring in Tunisia. J Basic Microbiol53:877–883 [CrossRef][PubMed]
    [Google Scholar]
  6. Chou J. H., Chou Y. J., Lin K. Y., Sheu S. Y., Sheu D. S., Arun A. B., Young C. C., Chen W. M.. 2007; Paenibacillus fonticola sp. nov., isolated from a warm spring. Int J Syst Evol Microbiol57:1346–1350 [CrossRef][PubMed]
    [Google Scholar]
  7. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E.. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol100:221–230 [CrossRef][PubMed]
    [Google Scholar]
  8. De Vos P., Ludwig W., Schleifer K. H., Whitman W. B.. 2009; Family IV. Paenibacillaceae fam. nov. In Bergey’s Manual of Systematic Bacteriology, 2nd edn.vol. 3 pp.269 Edited by De Vos P., Garrity G. M., Jones D., Krieg N. R., Ludwig W., Rainey F., Schleifer K. H., Whitman W. B.. New York: Springer;
    [Google Scholar]
  9. Dsouza M., Taylor M. W., Ryan J., MacKenzie A., Lagutin K., Anderson R. F., Turner S. J., Aislabie J.. 2014; Paenibacillus darwinianus sp. nov., isolated from gamma-irradiated Antarctic soil. Int J Syst Evol Bacteriol64:1406–1411 [CrossRef]
    [Google Scholar]
  10. Felsenstein J.. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution39:783–791 [CrossRef]
    [Google Scholar]
  11. Harper J. J., Davis G. H. G.. 1979; Two-dimensional thin-layer chromatography for amino acid analysis of bacterial cell walls. Int J Syst Bacteriol29:56–58 [CrossRef]
    [Google Scholar]
  12. Kim K. K., Lee K. C., Yu H., Ryoo S., Park Y., Lee J.-S.. 2010; Paenibacillus sputi sp. nov., isolated from the sputum of a patient with pulmonary disease. Int J Syst Evol Bacteriol60:2371–2376 [CrossRef]
    [Google Scholar]
  13. Kimura M.. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  14. Lee J.-S., Lee K. C., Chang Y.-H., Hong S. G., Oh H. W., Pyun Y.-R., Bae K. S.. 2002; Paenibacillus daejeonensis sp. nov., a novel alkaliphilic bacterium from soil. Int J Syst Evol Bacteriol52:2107–2111
    [Google Scholar]
  15. Logan N. A., Berge O., Bishop A. H., Busse H. J., De Vos P., Fritze D., Heyndrickx M., Kämpfer P., Rabinovitch L. et al. 2009; Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. Int J Syst Evol Microbiol59:2114–2121 [CrossRef][PubMed]
    [Google Scholar]
  16. Magee C. M., Rodeheaver G., Edgerton M. T., Edlich R. F.. 1975; A more reliable gram staining technic for diagnosis of surgical infections. Am J Surg130:341–346 [CrossRef][PubMed]
    [Google Scholar]
  17. Minnikin D. E., Collins M. D., Goodfellow M.. 1979; Fatty acid and polar lipid composition in the classification of cellulomonas, oerskovia and related taxa. J Appl Bacteriol47:87–95 [CrossRef]
    [Google Scholar]
  18. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. Journal of Microbiological Methods2:233–241 [CrossRef]
    [Google Scholar]
  19. Montes M. J., Mercadé E., Bozal N., Guinea J.. 2004; Paenibacillus antarcticus sp. nov., a novel psychrotolerant organism from the Antarctic environment. Int J Syst Evol Microbiol54:1521–1526 [CrossRef][PubMed]
    [Google Scholar]
  20. Nurkanto A., Julistiono H., Agusta A., Sjamsuridzal W.. 2012; Screening antimicrobial activity of actinomycetes isolated from raja ampat, west papua, indonesia. MAKARA J Science 16:21–26 [CrossRef]
    [Google Scholar]
  21. Osman S., Satomi M., Venkateswaran K.. 2006; Paenibacillus pasadenensis sp. nov. and Paenibacillus barengoltzii sp. nov., isolated from a spacecraft assembly facility. Int J Syst Evol Microbiol56:1509–1514 [CrossRef][PubMed]
    [Google Scholar]
  22. Saitou N., Nei M.. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol4:406–425[PubMed]
    [Google Scholar]
  23. Sasser M.. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  24. Schleifer K. H., Kandler O.. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev36:407–477[PubMed]
    [Google Scholar]
  25. Shida O., Takagi H., Kadowaki K., Nakamura L. K., Komagata K.. 1997; Transfer of Bacillus alginolyticus, Bacillus chondroitinus, Bacillus curdlanolyticus, Bacillus glucanolyticus, Bacillus kobensis, and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus . Int J Syst Bacteriol47:289–298 [CrossRef][PubMed]
    [Google Scholar]
  26. Stackbrandt E., Goebel B. M.. 1994; Taxonomic Note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol44:846–849 [CrossRef]
    [Google Scholar]
  27. Stansly P. G., Schlosser M. E.. 1947; Studies on polymyxin: isolation and identification of Bacillus polymyxa and differentiation of Polymyxin from certain known antibiotics. J Bacteriol54:549–556[PubMed]
    [Google Scholar]
  28. Takeda M., Suzuki I., Koizumi J. I.. 2005; Paenibacillus hodogayensis sp. nov., capable of degrading the polysaccharide produced by Sphaerotilus natans . Int J Syst Evol Microbiol55:737–741 [CrossRef][PubMed]
    [Google Scholar]
  29. Tamaoka J., Komagata K.. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett25:125–128 [CrossRef]
    [Google Scholar]
  30. Tang Q. Y., Yang N., Wang J., Xie Y. Q., Ren B., Zhou Y. G., Gu M. Y., Mao J., Li W. J. et al. 2011; Paenibacillus algorifonticola sp. nov., isolated from a cold spring. Int J Syst Evol Microbiol61:2167–2172 [CrossRef][PubMed]
    [Google Scholar]
  31. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res25:4876–4882 [CrossRef][PubMed]
    [Google Scholar]
  32. Tindall B. J., Rosselló-Móra R., Busse H. J., Ludwig W., Kämpfer P.. 2010; Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol60:249–266 [CrossRef][PubMed]
    [Google Scholar]
  33. Wang M., Yang M., Zhou G., Luo X., Zhang L., Tang Y., Fang C.. 2008; Paenibacillus tarimensis sp. nov., isolated from sand in Xinjiang, China. Int J Syst Evol Microbiol58:2081–2085 [CrossRef][PubMed]
    [Google Scholar]
  34. Xiang W., Wang G., Wang Y., Yao R., Zhang F., Wang R., Wang D., Zheng S.. 2014; Paenibacillus selenii sp. nov., isolated from selenium mineral soil. Int J Syst Evol Microbiol64:2662–2667 [CrossRef][PubMed]
    [Google Scholar]
  35. Yao R., Wang R., Wang D., Su J., Zheng S., Wang G.. 2014; Paenibacillus selenitireducens sp. nov., a selenite-reducing bacterium isolated from a selenium mineral soil. Int J Syst Evol Microbiol64:805–811 [CrossRef][PubMed]
    [Google Scholar]
  36. Zhou Y., Gao S., Wei D. Q., Yang L. L., Huang X., He J., Zhang Y. J., Tang S. K., Li W. J.. 2012; Paenibacillus thermophilus sp. nov., a novel bacterium isolated from a sediment of hot spring in Fujian province, China. Antonie Van Leeuwenhoek102:601–609 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001151
Loading
/content/journal/ijsem/10.1099/ijsem.0.001151
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error