1887

Abstract

The actinomycete strains K12-0408 and K12-0792 were isolated on CM-cellulose agar from rhizosphere soil of a pteridophytic plant collected in Tokyo prefecture, Japan. Their taxonomic positions were determined using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains K12-0408 and K12-0792 were positioned within the family . The strains formed extensively branched aerial and substrate mycelia. Long chains of cylindrical spores with smooth surfaces were formed on aerial hyphae. The cell wall contained diaminopimelic acid, and galactose, glucose, mannose and ribose were detected in whole-cell hydrolysates. The predominant menaquinones were MK-10(H) and MK-10(H). The major cellular fatty acids were anteiso-C and anteiso-C 2-OH. The DNA G+C contents of strains K12-0408 and K12-0792 were 69.6 and 69.7 mol%, respectively. Based on data from the present polyphasic taxonomic study, strains K12-0408 and K12-0792 represent a novel genus, for which the name gen. nov. is proposed, with strain K12-0408 (=NBRC 111897=TBRC 5327) as the type strain of the type species, sp. nov.

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2016-08-01
2019-12-13
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References

  1. Ara I., Kudo T.. 2007a; Luedemannella gen. nov., a new member of the family Micromonosporaceaeand description of Luedemannella helvata sp. nov. and Luedemannella flava sp. nov. J Gen Appl Microbiol53:39–51[CrossRef]
    [Google Scholar]
  2. Ara I., Kudo T.. 2007b; Krasilnikovia gen. nov., a new member of the family Micromonosporaceaeand description of Krasilnikovia cinnamonea sp. nov. Actinomycetologica21:1–10 [CrossRef]
    [Google Scholar]
  3. Ara I., Bakir M. A., Kudo T.. 2008a; Transfer of Catellatospora koreensis Lee, et al. 2000 as Catelliglobosispora koreensis gen. nov., comb. nov. and Catellatospora tsunoense Asano, et al. 1989 as Hamadaea tsunoensis gen. nov., comb. nov., and emended description of the genus Catellatospora Asano and Kawamoto 1986 emend. Lee and Hah 2002. Int J Syst Evol Microbiol58:1950–1960[CrossRef]
    [Google Scholar]
  4. Ara I., Matsumoto A., Bakir M. A., Kudo T., Ōmura S., Takahashi Y.. 2008b; Pseudosporangium ferrugineum gen. nov., sp. nov., a new member of the family Micromonosporaceae. Int J Syst Evol Microbiol58:1644–1652[CrossRef]
    [Google Scholar]
  5. Asano K., Kawamoto I.. 1986; Catellatospora, a new genus of the Actinomycetales. Int J Syst Evol Microbiol36:512–517
    [Google Scholar]
  6. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E.. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol100:221–230 [CrossRef][PubMed]
    [Google Scholar]
  7. Couch J. N.. 1950; Actinoplanes, a new genus of the Actinomycetales . J Elisha Mitchell Sci Soc66:87–92
    [Google Scholar]
  8. Ezaki T., Hashimoto Y., Yabuuchi E.. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol39:224–229[CrossRef]
    [Google Scholar]
  9. Felsenstein J.. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution39:783–791 [CrossRef]
    [Google Scholar]
  10. Felsenstein J.. 1993; PHYLIP (Phylogenetic Inference Package) Version 3.5c. Department of Genetics, University of Washington
  11. Fitch W. M.. 1971; Toward defining the course of evolution: minimal change for a specific tree topology. Syst Zool20:406–416[CrossRef]
    [Google Scholar]
  12. Gordon R. E., Mihm J. M.. 1957; A comparative study of some strains received as nocardiae. J Bacteriol73:15–27[PubMed]
    [Google Scholar]
  13. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N.. 1974; Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol24:54–63[CrossRef]
    [Google Scholar]
  14. Hasegawa T., Takizawa M., Tanida S.. 1983; A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol29:319–322[CrossRef]
    [Google Scholar]
  15. Inahashi Y., Matsumoto A., Danbara H., Ōmura S., Takahashi Y.. 2010; Phytohabitans suffuscus gen. nov., sp. nov., an actinomycete of the family Micromonosporaceae isolated from plant roots. Int J Syst Evol Microbiol60:2652–2658 [CrossRef][PubMed]
    [Google Scholar]
  16. Jacobson E., Grauville W. C., Fogs C. E.. 1958; Color Harmony Manual, 4th edn. Chicago: Container Corporation of America;
    [Google Scholar]
  17. Jia F., Liu C., Wang X., Zhao J., Liu Q., Zhang J., Gao R., Xiang W.. 2013; Wangella harbinensis gen. nov., sp. nov., a new member of the family Micromonosporaceae. Antonie Van Leeuwenhoek103:399–408 [CrossRef][PubMed]
    [Google Scholar]
  18. Kane W. D.. 1966; A new genus of Actinoplanaceae, Pilimelia, with a description of two species, Pilimelia terevasa and Pilimelia anulata . J Elisha Mitchell Sci Soc82:220–223
    [Google Scholar]
  19. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  20. Kimura M.. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol16:111–120[PubMed][CrossRef]
    [Google Scholar]
  21. Koch C., Kroppenstedt R. M., Rainey F. A., Stackebrandt E.. 1996; 16S ribosomal DNA analysis of the genera Micromonospora, Actinoplanes, Catellatospora, Catenuloplanes, Couchioplanes, Dactylosporangium, and Pilimelia and emendation of the family Micromonosporaceae . Int J Syst Bacteriol46:765–768 [CrossRef][PubMed]
    [Google Scholar]
  22. Krasil’nikov N. A.. 1938; Ray Fungi and Related Organisms-Actinomycetales Moscow: Akademii Nauk SSSR (in Russian);
    [Google Scholar]
  23. Kroppenstedt R. M.. 1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics pp173–199 Edited by Goodfellow M., Minnikin D. E.. London: Academic Press;
    [Google Scholar]
  24. Lechevalier M. P., Lechevalier H.. 1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol20:435–443[CrossRef]
    [Google Scholar]
  25. Lechevalier M. P., Stern A. E., Lechevalier H. A.. 1981; Phospholipids in the taxonomy of actinomycetes. Zentbl Bakteriol Hyg Abt1:111–116
    [Google Scholar]
  26. Lee D. W., Lee S. D.. 2011; Allocatelliglobosispora scoriae gen. nov., sp. nov., isolated from volcanic ash. Int J Syst Evol Microbiol61:264–270 [CrossRef][PubMed]
    [Google Scholar]
  27. Lee S. D., Hah Y. C.. 2002; Proposal to transfer Catellatospora ferruginea and 'Catellatospora ishikariense' to Asanoa gen. nov. as Asanoa ferruginea comb. nov. and Asanoa ishikariensis sp. nov., with emended description of the genus Catellatospora . Int J Syst Evol Microbiol52:967–972 [CrossRef][PubMed]
    [Google Scholar]
  28. Li J., Zhao G. Z., Zhu W. Y., Huang H. Y., Xu L. H., Zhang S., Li W. J.. 2011; Phytomonospora endophytica gen. nov., sp. nov., isolated from the roots of Artemisia annua L. Int J Syst Evol Microbiol61:2967–2973 [CrossRef][PubMed]
    [Google Scholar]
  29. Maldonado L. A., Fenical W., Jensen P. R., Kauffman C. A., Mincer T. J., Ward A. C., Bull A. T., Goodfellow M.. 2005; Salinispora arenicola gen. nov., sp. nov. and Salinispora tropica sp. nov., obligate marine actinomycetes belonging to the family Micromonosporaceae. Int J Syst Evol Microbiol55:1759–1766 [CrossRef][PubMed]
    [Google Scholar]
  30. Matsumoto A., Takahashi Y., Shinose M., Seino A., Iwai Y., Omura S.. 2003; Longispora albida gen. nov., sp. nov., a novel genus of the family Micromonosporaceae. Int J Syst Evol Microbiol53:1553–1559 [CrossRef][PubMed]
    [Google Scholar]
  31. Matsumoto A., Kawaguchi Y., Nakashima T., Iwatsuki M., Ōmura S., Takahashi Y.. 2014; Rhizocola hellebori gen. nov., sp. nov., an actinomycete of the family Micromonosporaceae containing 3,4-dihydroxydiaminopimelic acid in the cell-wall peptidoglycan. Int J Syst Evol Microbiol64:2706–2711 [CrossRef][PubMed]
    [Google Scholar]
  32. Medina P., Baresi L.. 2007; Rapid identification of gelatin and casein hydrolysis using TCA. J Microbiol Methods69:391–393 [CrossRef][PubMed]
    [Google Scholar]
  33. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M.. 1977; Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol27:104–117[CrossRef]
    [Google Scholar]
  34. Monciardini P., Cavaletti L., Ranghetti A., Schumann P., Rohde M., Bamonte R., Sosio M., Mezzelani A., Donadio S.. 2009; Novel members of the family Micromonosporaceae, Rugosimonospora acidiphila gen. nov., sp. nov. and Rugosimonospora africana sp. nov. Int J Syst Evol Microbiol59:2752–2758 [CrossRef][PubMed]
    [Google Scholar]
  35. Ørskov J.. 1923; Investigations Into the Morphology of the Ray Fungi Copenhagen, Denmark: Levin and Munksgaard;
    [Google Scholar]
  36. Qin S., Li J., Zhang Y. Q., Zhu W. Y., Zhao G. Z., Xu L. H., Li W. J.. 2009; Plantactinospora mayteni gen. nov., sp. nov., a member of the family Micromonosporaceae. Int J Syst Evol Microbiol59:2527–2533 [CrossRef][PubMed]
    [Google Scholar]
  37. Rheims H., Schumann P., Rohde M., Stackebrandt E.. 1998; Verrucosispora gifhornensis gen. nov., sp. nov., a new member of the actinobacterial family Micromonosporaceae. Int J Syst Evol Microbiol48:1119–1127
    [Google Scholar]
  38. Sahin N., Veyisoglu A., Tatar D., Saygin H., Cetin D., Guven K., Klenk H. P., Goodfellow M.. 2015; Phytomonospora cypria sp. nov., isolated from soil. Antonie Van Leeuwenhoek108:1425–1432 [CrossRef][PubMed]
    [Google Scholar]
  39. Saito H., Miura K. I.. 1963; Preparation of transforming deoxyribonucleic acid by phenoltreatment. Biochim Biophys Acta72:619–629[PubMed][CrossRef]
    [Google Scholar]
  40. Saitou N., Nei M.. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol4:406–425[PubMed]
    [Google Scholar]
  41. Shirling E. B., Gottlieb D.. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol16:313–340[CrossRef]
    [Google Scholar]
  42. Stackebrandt E., Rainey F. A., Ward-Rainey N. L.. 1997; Proposal for a new Hierarchic classification system, Actinobacteria classis nov. Int J Syst Evol Microbiol47:479–491
    [Google Scholar]
  43. Staneck J. L., Roberts G. D.. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol28:226–231[PubMed]
    [Google Scholar]
  44. Také A., Matsumoto A., Ōmura S., Takahashi Y.. 2015; Streptomyces lactacystinicus sp. nov. and Streptomyces cyslabdanicus sp. nov., producing lactacystin and cyslabdan, respectively. J Antibiot68:322–327 [CrossRef][PubMed]
    [Google Scholar]
  45. Tamaoka J., Komagata K.. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett25:125–128 [CrossRef]
    [Google Scholar]
  46. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. 2013; MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  47. Tamura T., Nakagaito Y., Nishii T., Hasegawa T., Stackebrandt E., Yokota A.. 1994; A new genus of the order Actinomycetales Couchioplanes gen. nov., with descriptions of Couchioplanes caeruleus (Horan and Brodsky 1986) comb. nov. and Couchioplanes caeruleus subsp. azureus subsp. nov. Int J Syst Bacteriol44:193–203 [CrossRef][PubMed]
    [Google Scholar]
  48. Tamura T., Hayakawa M., Hatano K.. 1997; A new genus of the order Actinomycetales, Spirilliplanes gen. nov., with description of Spirilliplanes yamanashiensis sp. nov. Int J Syst Evol Microbiol47:97–102
    [Google Scholar]
  49. Tamura T., Hayakawa M., Hatano K.. 2001; A new genus of the order Actinomycetales, Virgosporangium gen. nov., with descriptions of Virgosporangium ochraceum sp. nov. and Virgosporangium aurantiacum sp. nov. Int J Syst Evol Microbiol51:1809–1816 [CrossRef][PubMed]
    [Google Scholar]
  50. Tamura T., Hatano K., Suzuki K.. 2006; A new genus of the family Micromonosporaceae, Polymorphospora gen. nov., with description of Polymorphospora rubra sp. nov. Int J Syst Evol Microbiol56:1959–1964 [CrossRef][PubMed]
    [Google Scholar]
  51. Thawai C., Tanasupawat S., Itoh T., Kudo T.. 2006; Actinocatenispora thailandica gen. nov., sp. nov., a new member of the family Micromonosporaceae. Int J Syst Evol Microbiol56:1789–1794 [CrossRef][PubMed]
    [Google Scholar]
  52. Thiemann J. E., Pagani H., Beretta G.. 1967; A new genus of the Actinoplanaceae: Dactylosporanguim, gen. nov. Archiv FüR Mikrobiologie58:42–52 [CrossRef][PubMed]
    [Google Scholar]
  53. Tomiyasu I.. 1982; Mycolic acid composition and thermally adaptative changes in Nocardia asteroides . J Bacteriol151:828–837[PubMed]
    [Google Scholar]
  54. Uchida K., Aida K.. 1977; Acyl type of bacterial cell wall: its simple identification by a colorimetric method. J Gen Appl Microbiol23:249–260[CrossRef]
    [Google Scholar]
  55. Wang X., Jia F., Liu C., Zhao J., Wang L., Shen Y., Wang J., Zhang J., Li C. et al. 2013; Xiangella phaseoli gen. nov., sp. nov., a member of the family Micromonosporaceae. Int J Syst Evol Microbiol63:2138–2145 [CrossRef][PubMed]
    [Google Scholar]
  56. Wiese J., Jiang Y., Tang S. K., Thiel V., Schmaljohann R., Xu L. H., Jiang C. L., Imhoff J. F.. 2008; A new member of the family Micromonosporaceae, Planosporangium flavigriseum gen. nov., sp. nov. Int J Syst Evol Microbiol58:1324–1331 [CrossRef][PubMed]
    [Google Scholar]
  57. Xie Q. Y., Wang C., Wang R., Qu Z., Lin H. P., Goodfellow M., Hong K.. 2011; Jishengella endophytica gen. nov., sp. nov., a new member of the family Micromonosporaceae. Int J Syst Evol Microbiol61:1153–1159 [CrossRef][PubMed]
    [Google Scholar]
  58. Yokota A., Tamura T., Hasegawa T., Huang L. H.. 1993; Catenuloplanes japonicus gen. nov., sp. nov., nom. rev., a new genus of the order Actinomycetales . Int J Syst Evol Microbiol43:805–812
    [Google Scholar]
  59. Zhi X. Y., Li W. J., Stackebrandt E.. 2009; An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria, with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. Int J Syst Evol Microbiol59:589–608 [CrossRef][PubMed]
    [Google Scholar]
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