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Abstract

A novel Gram-stain-negative bacterium, named strain NSCS20N07D, was isolated from surface seawater of the South China Sea. Cells of this strain contained poly-β-hydroxybutyrate granules. Growth was observed at 15–35 °C with optimum of 30 °C, at a salinity range of 1–6 % (w/v) NaCl with optimum of 3 % and at pH 5–8 with optimum of pH 5. The full-length 16S rRNA gene sequence of strain NSCS20N07D showed highest similarity to Photobacterium iliopiscarium ATCC 51760 of 96.0 %, followed by Photobacterium kishitanii pjapo.1.1, Photobacterium phosphoreum ATCC 11040 and Photobacterium aquimaris LC2-065 of 96.0, 95.8 and 95.7 %, respectively. Phylogenetic analysis showed that strain NSCS20N07D formed a separate clade distinct from species of the genus Photobacterium and other genera within the family Vibrionaceae , indicating that strain NSCS20N07D represented a novel genus affiliated with this family. The genome size of strain NSCS20N07D was 2.5 Mb, which was much smaller than those of related species in the family Vibrionaceae . The predominant fatty acids were C16 : 0, C17 : 0 cyclo, C19 : 0 cyclo ω8c, C18 : 0 and summed feature 2 (C14 : 0 3-OH/iso-C16 : 1 I). The respiratory quinone was Q-8. The polar lipids were identified as phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and four unidentified lipids. The DNA G+C content was 30.7 mol%. Combined, these results suggest that strain NSCS20N07D represents a novel species of a new genus, for which the name Paraphotobacterium marinum gen. nov., sp. nov. is proposed. The type strain of Paraphotobacterium marinum is NSCS20N07D(=KCTC 52126=MCCC 1A01886=CIP 111031).

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2016-08-01
2019-10-14
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References

  1. Bankevich A. , Nurk S. , Antipov D. , Gurevich A. A. , Dvorkin M. , Kulikov A. S. , Lesin V. M. , Nikolenko S. I. , Pham S. et al. ( 2012;). SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. . J Comput Biol 19: 455–477. [CrossRef] [PubMed]
    [Google Scholar]
  2. Chamroensaksri N. , Tanasupawat S. , Akaracharanya A. , Visessanguan W. , Kudo T. , Itoh T. . ( 2009;). Salinivibrio siamensis sp. nov., from fermented fish (pla-ra) in Thailand. . Int J Syst Evol Microbiol 59: 880–885. [CrossRef] [PubMed]
    [Google Scholar]
  3. Choi A. , Kim K. M. , Kang I. , Youn S. H. , Suh Y. S. , Lee Y. , Cho J. C. . ( 2012;). Grimontia marina sp. nov., a marine bacterium isolated from the Yellow Sea. . J Microbiol 50: 170–174. [CrossRef] [PubMed]
    [Google Scholar]
  4. DeLong E. F. . ( 1992;). Archaea in coastal marine environments. . Proc Natl Acad Sci U S A 89: 5685–5689.[PubMed] [CrossRef]
    [Google Scholar]
  5. Denner E. B. , Vybiral D. , Fischer U. R. , Velimirov B. , Busse H. J. . ( 2002;). Vibrio calviensis sp. nov., a halophilic, facultatively oligotrophic 0.2 microm-fiIterabIe marine bacterium. . Int J Syst Evol Microbiol 52: 549–553. [CrossRef] [PubMed]
    [Google Scholar]
  6. Gomez-Gil B. , Thompson C, C. , Matsumura Y. , Sawabe T. , Iida T. , Christen R. , Thompson F. , Sawabe T. . ( 2014;). The Famlily Vibrionaceae. . In The Prokaryotes, , 4th edn., pp. 659–747. Edited by Rosenberg E. , Delong E. F. , Thonpson F. L. , Lory S. , Stackebrandnt E. . New York:: Springer;.
    [Google Scholar]
  7. Gurevich A. , Saveliev V. , Vyahhi N. , Tesler G. . ( 2013;). QUAST: quality assessment tool for genome assemblies. . Bioinformatics 29: 1072–1075. [CrossRef] [PubMed]
    [Google Scholar]
  8. Hendrie M. , Hodgkiss W. , Shewan J. . ( 1971;). Proposal that Vibrio marinus (Russell 1891) Ford 1927 be Amalgamated with Vibrio fischeri (Beijerinck 1889) Lehmann and Neumann 1896. . Int J Syst Bacteriol 21: 217–221.[CrossRef]
    [Google Scholar]
  9. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. et al. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62: 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  10. Kim Y. O. , Kim K. K. , Park S. , Kang S. J. , Lee J. H. , Lee S. J. , Oh T. K. , Yoon J. H. . ( 2010;). Photobacterium gaetbulicola sp. nov., a lipolytic bacterium isolated from a tidal flat sediment. . Int J Syst Evol Microbiol 60: 2587–2591. [CrossRef] [PubMed]
    [Google Scholar]
  11. Komagata K. , Suzuki K. . ( 1987;). Lipid and cell-wall analysis in bacterial systematics. . Method Microbiol 19: 161–207.[CrossRef]
    [Google Scholar]
  12. Lo N. , Jin H. M. , Jeon C. O. . ( 2014;). Photobacterium aestuarii sp. nov., a marine bacterium isolated from a tidal flat. . Int J Syst Evol Microbiol 64: 625–630. [CrossRef] [PubMed]
    [Google Scholar]
  13. Nedashkovskaya O. I. , Stenkova A. M. , Zhukova N. V. , Van Trappen S. , Lee J. S. , Kim S. B. . ( 2013;). Echinimonas agarilytica gen. nov., sp. nov., a new gammaproteobacterium isolated from the sea urchin Strongylocentrotus intermedius . . Antonie Van Leeuwenhoek 103: 69–77. [CrossRef] [PubMed]
    [Google Scholar]
  14. Nogi Y. , Masui N. , Kato C. . ( 1998;). Photobacterium profundum sp. nov., a new, moderately barophilic bacterial species isolated from a deep-sea sediment. . Extremophiles 2: 1–7. [CrossRef] [PubMed]
    [Google Scholar]
  15. Ostle A. G. , Holt J. G. . ( 1982;). Nile blue a as a fluorescent stain for poly-beta-hydroxybutyrate. . Appl Environ Microbiol 44: 238–241.[PubMed]
    [Google Scholar]
  16. Pascual J. , Macián M. C. , Arahal D. R. , Garay E. , Pujalte M. J. . ( 2009;). Description of Enterovibrio nigricans sp. nov., reclassification of Vibrio calviensis as Enterovibrio calviensis comb. nov. and emended description of the genus Enterovibrio Thompson et al. 2002. . Int J Syst Evol Microbiol 59: 698–704. [CrossRef] [PubMed]
    [Google Scholar]
  17. Romano I. , Gambacorta A. , Lama L. , Nicolaus B. , Giordano A. . ( 2005;). Salinivibrio costicola subsp. alcaliphilus subsp. nov., a haloalkaliphilic aerobe from Campania Region (Italy). . Syst Appl Microbiol 28: 34–42. [CrossRef] [PubMed]
    [Google Scholar]
  18. Sawabe T. , Ogura Y. , Matsumura Y. , Feng G. , Amin A. R. , Mino S. , Nakagawa S. , Sawabe T. , Kumar R. et al. ( 2013;). Updating the Vibrio clades defined by multilocus sequence phylogeny: proposal of eight new clades, and the description of Vibrio tritonius sp. nov. . Front Microbiol 4: 414. [CrossRef] [PubMed]
    [Google Scholar]
  19. Tamura K. , Stecher G. , Peterson D. , Filipski A. , Kumar S. . ( 2013;). MEGA6: molecular evolutionary genetics analysis version 6.0. . Mol Biol Evol 30: 2725–2729. [CrossRef] [PubMed]
    [Google Scholar]
  20. Tanaka T. , Kawasaki K. , Daimon S. , Kitagawa W. , Yamamoto K. , Tamaki H. , Tanaka M. , Nakatsu C. H. , Kamagata Y. . ( 2014;). A hidden pitfall in the preparation of agar media undermines microorganism cultivability. . Appl Environ Microbiol 80: 7659–7666. [CrossRef] [PubMed]
    [Google Scholar]
  21. Yoshizawa S. , Wada M. , Kita-Tsukamoto K. , Yokota A. , Kogure K. . ( 2009;). Photobacterium aquimaris sp. nov., a luminous marine bacterium isolated from seawater. . Int J Syst Evol Microbiol 59: 1438–1442. [CrossRef] [PubMed]
    [Google Scholar]
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