1887

Abstract

A novel, Gram-stain-negative, motile, facultatively anaerobic bacterium, designated strain NF48, was isolated from surface seawater around Zhoushan Islands. Cells were rod-shaped (1.1–3.9×0.5–0.9 µm). Strain NF48was able to grow at 10–40 °C (optimum 30 °C), at pH 5.5–9.0 (optimum 6.5–8.0) and with 0.5–7.0 % (w/v) NaCl (optimum 2.0 %). The genomic DNA G+C content was 65.5 mol%. Chemotaxonomic analysis showed that the main isoprenoid quinone was Q-10 and the major fatty acids were C 7 and C. The major polar lipids of strain NF48were phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NF48 belonged to the genus with similarity values of 95.5–97.2 % to members of this genus, and was most closely related to SS011A0-7#2-2 (97.2 %). On the basis of the phenotypic, chemotaxonomic and phylogenetic characteristics, strain NF48is suggested to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NF48 (=MCCC 1K01157=KCTC 42650).

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2016-08-01
2019-12-11
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References

  1. Embley T. M.. 1991; The linear PCR reaction: a simple and robust method for sequencing amplified rRNA genes. Lett Appl Microbiol13:171–174 [CrossRef][PubMed]
    [Google Scholar]
  2. Felsenstein J.. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  3. Fitch W. M.. 1971; Toward Defining the Course of Evolution: Minimum Change for a Specific Tree Topology. Syst Zool20:406–416 [CrossRef]
    [Google Scholar]
  4. Hu J., Yang Q. Q., Ren Y., Zhang W. W., Zheng G., Sun C., Pan J., Zhu X. F., Zhang X. Q. et al. 2015; Maribacter thermophilus sp. nov., isolated from an algal bloom in an intertidal zone, and emended description of the genus Maribacter. Int J Syst Evol Microbiol65:36–41 [CrossRef][PubMed]
    [Google Scholar]
  5. Jia Y. Y., Sun C., Pan J., Zhang W. Y., Zhang X. Q., Huo Y. Y., Zhu X. F., Wu M.. 2014; Devosia pacifica sp. nov., isolated from deep-sea sediment. Int J Syst Evol Microbiol64:2637–2641 [CrossRef][PubMed]
    [Google Scholar]
  6. Kim M., Oh H. S., Park S. C., Chun J.. 2014; Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol64:346–351 [CrossRef][PubMed]
    [Google Scholar]
  7. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  8. Kimura M.. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  9. Komagata K., Suzuki K. I.. 1987; Lipid and cell-wall analysis in bacterial systematics. J Methods Microbiol19:1
    [Google Scholar]
  10. Kuykendall L., Roy M., O'neill J., Devine T.. 1988; Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum. Int J Syst Evol Microbiol38:358–361 [CrossRef]
    [Google Scholar]
  11. Leifson E.. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol85:[PubMed]
    [Google Scholar]
  12. Mesbah M., Whitman W. B.. 1989; Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. J Chromatogr479:297–306 [CrossRef][PubMed]
    [Google Scholar]
  13. Minnikin D., Patel P., Alshamaony L., Goodfellow M.. 1977; Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Evol Microbiol27:104–117 [CrossRef]
    [Google Scholar]
  14. Oppenheimer C.. 1952; The growth and viability of sixty-three species of marine bacteria as influenced by hydrostatic pressure. J Mar Res11:10–18
    [Google Scholar]
  15. Pan J., Sun C., Zhang X. Q., Huo Y. Y., Zhu X. F., Wu M.. 2014; A novel species from marine sediment of Pacific Ocean as Paracoccus sediminis sp. nov. Int J Syst Evol Microbiol27:104–117
    [Google Scholar]
  16. Saitou N., Nei M.. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol4:406–425[PubMed]
    [Google Scholar]
  17. Smibert R.. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology , pp.607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R.. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  18. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. 2011; mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol28:2731–2739 [CrossRef][PubMed]
    [Google Scholar]
  19. Wu X. Y., Zheng G., Zhang W. W., Xu X. W., Wu M., Zhu X. F.. 2010; Amphibacillus jilinensis sp. nov., a facultatively anaerobic, alkaliphilic bacillus from a soda lake. Int J Syst Evol Microbiol60:2540–2543 [CrossRef][PubMed]
    [Google Scholar]
  20. Xian S., Zhang R., Sun J., Chen Y., Deng W., Li S., Jiao N.. 2014; Seohaeicola westpacificensis sp. nov., a novel member of genera Seohaeicola isolated from deep West Pacific Sea water. Curr Microbiol69:32–36 [CrossRef][PubMed]
    [Google Scholar]
  21. Xie B. S., Lv X. L., Cai M., Tang Y. Q., Wang Y. N., Cui H. L., Liu X. Y., Tan Y., Wu X. L.. 2014; Seohaeicola nanhaiensis sp. nov., a moderately halophilic bacterium isolated from the benthic sediment of South China Sea. Curr Microbiol69:802–808 [CrossRef][PubMed]
    [Google Scholar]
  22. Yoon J. H., Kang S. J., Lee S. Y., Oh K. H., Oh T. K.. 2009; Seohaeicola saemankumensis gen. nov., sp. nov., isolated from a tidal flat. Int J Syst Evol Microbiol59:2675–2679 [CrossRef][PubMed]
    [Google Scholar]
  23. Zhu X. F., Jia X. M., Zhang X. Q., Wu Y. H., Chen Z. Y.. 2011; Modern Experimental Technique of Microbiology Hangzhou: Zhejiang University Press (English translation);
    [Google Scholar]
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