1887

Abstract

A novel dissimilatory Fe(III)-reducing bacterium, designated strain GSS09, was isolated from a compost sample by using a solid medium containing acetate and ferrihydrite as electron donor and electron acceptor, respectively. Cells of strain GSS09 were anaerobic, Gram-stain-positive, motile, endospore-forming and rod-shaped. Growth occurred at 30–55 °C (optimum 50 °C), at pH 6.5–9.0 (optimum pH 7.5) and in the presence of 0–3 % (w/v) NaCl (optimum 1 %). Both sulfur compounds such as sulfate, sulfite and thiosulfate and Fe(III) oxides such as ferrihydrite could be utilized as electron acceptors. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain GSS09 was related closely to Desulfotomaculum hydrothermale Lam5 (94.5 % sequence similarity). The major fatty acids were C16 : 0 and C16 : 1 ω7c/C16 : 1 ω6c. The G+C content of the genomic DNA was 49.1 mol%. On the basis of phylogenetic analysis, phenotypic characterization and physiological tests, strain GSS09 is considered to represent a novel species of the genus Desulfotomaculum, for which the name Desulfotomaculum ferrireducens sp. nov. is proposed. The type strain is GSS09 (=KCTC 15523=MCCC 1K01254).

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2016-08-01
2019-12-09
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