1887

Abstract

A polyphasic approach was used to characterize an endospore-forming bacterium, designated strain CC-RT-E, isolated from resin fragments. Strain CC-RT-E was Gram-stain-variable and facultatively anaerobic, able to grow at 20–37 °C (optimal 30 °C) and pH6.0–9.0 (optimal pH 7.0). Ellipsoidal and terminal endospores were observed. The 16S rRNA gene sequence analysis of strain CC-RT-E showed highest sequence similarity to Ammoniphilus oxalivorans RAOx-FS (97.8 %) and Ammoniphilus oxalaticus RAOx-1 (97.7 %). According to the DNA–DNA hybridization, the relatedness values of strain CC-RT-E with Ammoniphilus oxalivorans RAOx-FS and Ammoniphilus oxalaticus RAOx-1 were 21 % and 29 %, respectively. The DNA G+C content was 39.2 mol% and the predominant quinone system was menaquinone-7 (MK-7). The major polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylmethylethanolamine. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The polyamine pattern showed predominance of sym-homospermidine and putrescine. The major fatty acids found in strain CC-RT-E were C14 : 0, iso-C15 : 0, anteiso-C15 : 0 and C16 : 1 ω7c/C16 : 1 ω6c. Based on the distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence analysis, strain CC-RT-E is considered to represent a novel species of the genus Ammoniphilus , for which the name Ammoniphilus resinaesp. nov. is proposed. The type strain is CC-RT-E (=BCRC 80314=DSM 24738).

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2016-08-01
2019-10-20
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