1887

Abstract

Two isolates of a Gram-stain-positive, strictly aerobic, motile, rod-shaped, endospore-forming bacterium were identified during a survey of the diversity of the Agriculture Research Service Culture Collection. These strains were originally isolated from soil and have a phenotype of producing a dark pigment on tryptic soy agar. Phylogenetic analysis of the 16S rRNA gene indicated that these strains were related most closely to subsp. (99.7 % similarity) and (99.7 %). In phenotypic characterization, the novel strains were found to grow between 17 and 50 °C and can tolerate up to 9 % (w/v) NaCl. Furthermore, the strains grew in media of pH 5.5–10 (optimal growth at pH 7.0–8.0). The predominant cellular fatty acids were anteiso-C (34.8 %) and iso-C (21.9 %). The cell-wall peptidoglycan contained -diaminopimelic acid. A draft genome of both strains was completed. The DNA G+C content was 43.8 mol%. A phylogenomic analysis on the core genome of these two new strains and all members of the group revealed these two strains formed a distinct monophyletic clade with the nearest neighbour . DNA–DNA relatedness studies using DNA–DNA hybridizations showed the two strains were conspecific (93.8 %), while values with all other species (<31.5 %) were well below the species threshold of 70 %. Based on the consensus of phylogenetic and phenotypic analyses, these strains are considered to represent a novel species within the genus , for which the name sp. nov. is proposed, with type strain NRRL B-41091 (=CCUG 68786).

Keyword(s): black and Pigment
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2016-08-01
2020-04-07
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References

  1. Auch A. F., von Jan M., Klenk H. P., Göker M.. 2010; Digital DNA-DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison. Stand Genomic Sci2:117–134 [CrossRef][PubMed]
    [Google Scholar]
  2. Borriss R., Chen X. H., Rueckert C., Blom J., Becker A., Baumgarth B., Fan B., Pukall R., Schumann P. et al. 2011; Relationship of Bacillus amyloliquefaciens clades associated with strains DSM 7T and FZB42T: a proposal for Bacillus amyloliquefaciens subsp. amyloliquefaciens subsp. nov. and Bacillus amyloliquefaciens subsp. plantarum subsp. nov. based on complete genome sequence comparisons. Int J Syst Evol Microbiol61:1786–1801 [CrossRef][PubMed]
    [Google Scholar]
  3. Breznak J. A., Costilow R. N.. 1994; Physicochemical factors in growth. Methods for General and Molecular Bacteriology137–154
    [Google Scholar]
  4. Burke S. A., Wright J. D., Robinson M. K., Bronk B. V., Warren R. L.. 2004; Detection of molecular diversity in Bacillus atrophaeus by amplified fragment length polymorphism analysis. Appl Environ Microbiol70:2786–2790 [CrossRef][PubMed]
    [Google Scholar]
  5. Jolley K. A., Maiden M. C.. 2010; BIGSdb: Scalable analysis of bacterial genome variation at the population level. BMC Bioinformatics11:595 [CrossRef][PubMed]
    [Google Scholar]
  6. Meier-Kolthoff J. P., Auch A. F., Klenk H. P., Göker M.. 2013; Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics14: [CrossRef][PubMed]
    [Google Scholar]
  7. Nakamura L. K.. 1989; Taxonomic relationship of black-pigmented Bacillus subtilis strains and a proposal for Bacillus atrophaeus sp. nov . Int J Syst Bacteriol39:295–300 [CrossRef]
    [Google Scholar]
  8. Rooney A. P., Price N. P. J., Ehrhardt C., Swezey J. L., Bannan J. D.. 2009; Phylogeny and molecular taxonomy of the Bacillus subtilis species complex and description of Bacillus subtilis subsp. inaquosorum subsp. nov . Int J Syst Evol Microbiol59:2429–2436 [CrossRef][PubMed]
    [Google Scholar]
  9. Ruiz-García C., Béjar V., Martínez-Checa F., Llamas I., Quesada E.. 2005; Bacillus velezensis sp. nov., a surfactant-producing bacterium isolated from the river Vélez in Málaga, southern Spain. Int J Syst Evol Microbiol55:191–195 [CrossRef][PubMed]
    [Google Scholar]
  10. Staneck J. L., Roberts G. D.. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol28:226–231[PubMed]
    [Google Scholar]
  11. Tamura K., Nei M.. 1993; Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol10:512–526[PubMed]
    [Google Scholar]
  12. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  13. Vos P., Garrity G., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K. H., Whitman W. B.. 2009; The Firmicutes. In Bergey's Manual of Systematic Bacteriology, 2nd edn.vol 3
    [Google Scholar]
  14. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol37:463–464[CrossRef]
    [Google Scholar]
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