1887

Abstract

A moderately halophilic, Gram-stain-negative, non-endospore-forming endophytic bacterium designated strain ST307 was isolated from the euhalophyte Suaeda salsa in Dongying, China. Strain ST307 was aerobic, rod-shaped, motile and orange–yellow-pigmented. The organism grew at NaCl concentrations of 0.6–20 % (w/v) (optimum 5–6 %, w/v), at temperatures of 5–45 °C (optimum 35 °C) and at pH 5–9 (optimum pH 7–8). It accumulated poly-β-hydroxybutyric acid and produced exopolysaccharides. The major fatty acids were C18 : 1 ω7c/C18 : 1 ω6c, C16 : 0 and C16 : 1 ω7c/C16 : 1 ω6c. The predominant lipoquinone was ubiquinone Q-9. The polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, a glycoaminolipid and a phosphoglycoaminolipid. The DNA G+C content was 60.5 mol%. Phylogenetic analyses of 16S rRNA gene sequences and concatenated atpA, rpoD and secA gene sequences revealed that the strain represents a member of the genus Larsenimonas . The closest related type strain was Larsenimonas salina M1-18. Mean DNA–DNA relatedness values between strain ST307 and the related species L. salina M1-18, Chromohalobacter beijerinckii DSM 7218, C. canadensis DSM 6769, C. israelensis DSM 6768, C. marismortui CGMCC 1.2321, C. nigrandesensis DSM 14323, C. salexigens DSM 3043 and C. sarecensis DSM 15547 were 15±2−45±1 %. On the basis of phenotypic, chemotaxonomic and molecular features, strain ST307 clearly represents a novel species of the genus Larsenimonas . The name Larsenimonas suaedae sp. nov. is proposed, with ST307 (=CGMCC 1.8902=DSM 22428) as the type strain.

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2016-08-01
2019-10-23
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