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Abstract

Strictly anaerobic bacterial strains (FH052 and SN021) belonging to clostridial cluster XIVa were isolated from a methanogenic reactor. Cells of the two strains were Gram-stain-positive, slender or curved rods producing terminal spores. The strains were slightly alkaliphilic. They fermented various carbohydrates including xylan and produced acetate, ethanol and H. Strain SN021 decomposed cellulose. The genomic DNA G+C contents were 47.2 mol% for strain FH052 and 38.1 mol% for strain SN021. The two strains had common cellular fatty acids such as C, C dimethylacetal and C 7 dimethylacetal as major components. 16S rRNA gene sequence similarity between the two strains was 94.3 % and they shared closely related species such as , and (92.6–95.7 % sequence similarity). Phenotypic and phylogenetic analyses suggested that these two isolates should be assigned to novel genera other than the genus , and thus the genera gen. nov. and gen. nov. in the family were proposed to accommodate them as gen. nov., sp. nov. for strain FH052 (=JCM 30820=DSM 100431) and gen. nov., sp. nov. for strain SN021 (=JCM 30819=DSM 100423). For the three related species, DSM 1283 (=JCM 11016=ATCC 13725) comb. nov., HY-35-12 (=DSM 15929=KCTC 5026) comb. nov. and strain HESP1 (=DSM 12503=JCM 31057) comb. nov. are proposed with emended descriptions of these species.

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2016-08-01
2020-01-23
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