1887

Abstract

Gram-stain-positive, partially acid-fast, non-spore-forming, anaerobic, catalase-positive, pleomorphic bacteria were isolated from human abscesses. Strains X1036, X1698 and NML 120705, were recovered from a spinal abscess, a peritoneal abscess and a breast abscess respectively. A phylogenetic analysis of the 16S rRNA gene sequences showed that the strains shared 100 % similarity, and the nearest phylogenetic neighbour was DSM 45568 (95%). Chemotaxonomic characteristics of the strains were consistent with those described for members of the suborder . Mycolic acids were detected using HPLC and one-dimensional TLC; whole-cell hydrolysates yielded -diaminopimelic acid with arabinose and galactose as the predominant sugars; the muramic acid acyl type was acetylated; the major menaquinone was MK-9 (96.3%); polar lipids detected were phosphatidylglycerol, phosphatidylinositol and an unknown glycophospholipid. Cellular fatty acids were hexadecanoic acid (C), octadecenoic acid (Cω9) and decanoic acid (C). Tuberculostearic acid was not detected. Based on the results of this polyphasic study, we conclude that these strains represent a novel genus and species within the suborder for which we propose the name gen. nov., sp. nov., with the type strain X1036 (=DSM 45743=CCUG 66657).

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2016-08-01
2020-09-29
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