@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001118, author = "Song, Hye Seon and Cha, In-Tae and Rhee, Jin-Kyu and Yim, Kyung June and Kim, Ah Yoon and Choi, Jong-Soon and Baek, Su Jeong and Seo, Myung-Ji and Park, Soo-Je and Nam, Young-Do and Roh, Seong Woon", title = "Halostella salina gen. nov., sp. nov., an extremely halophilic archaeon isolated from solar salt", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2016", volume = "66", number = "7", pages = "2740-2746", doi = "https://doi.org/10.1099/ijsem.0.001118", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001118", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "polyphasic taxonomy", keywords = "haloarchaea", keywords = "solar salt", keywords = "Halostella salina", keywords = "Halobacteriales", abstract = "A novel halophilic archaeon designated strain CBA1114T was isolated from solar salt in the Republic of Korea. Strain CBA1114T, cells of which were coccoid and Gram-stain-negative, grew in the presence of 15–30 % (w/v) NaCl (optimum, 20 %) and at 20–50 °C (optimum, 40 °C) and pH 7.0–9.0 (optimum, pH 8.0). Strain CBA1114T required Mg2+ for growth. Strain CBA1114T had three 16S rRNA genes, rrnA, rrnB and rrnC; levels of similarity between the sequences were 99.7–99.9 %. The 16S rRNA gene sequence of strain CBA1114T showed 91.7 % similarity to that of Haloterrigena thermotolerans PR5T. In multilocus sequence analysis (MLSA), five housekeeping genes, atpB, EF-2, radA, rpoB′ and secY, were found to be closely related to those of the members of the genera Halorientalis (89.7 % similarity of the atpB gene sequence), Halomicroarcula (91.9 %, EF-2), Haloterrigena (85.4 %, radA), Natronoarchaeum (89.2 %, rpoB′) and Natrinema (75.7 %, secY). A phylogenetic tree generated from the results of MLSA of the five housekeeping genes showed that strain CBA1114T was closely related to species of the genus Halorientalis in the family Halobacteriaceae . The major polar lipids were identified as phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and unidentified lipids. The G+C content of the genomic DNA of strain CBA1114T was 68.1 mol%. According to the results of phylogenetic, phenotypic and chemotaxonomic analyses, we designate strain CBA1114T (=JCM 30111T=KCTC 4206T) as the type strain of Halostella salina gen. nov., sp. nov., a novel species of a new genus within the family Halobacteriaceae .", }