1887

Abstract

A novel halophilic archaeon designated strain CBA1114 was isolated from solar salt in the Republic of Korea. Strain CBA1114, cells of which were coccoid and Gram-stain-negative, grew in the presence of 15–30 % (w/v) NaCl (optimum, 20 %) and at 20–50 °C (optimum, 40 °C) and pH 7.0–9.0 (optimum, pH 8.0). Strain CBA1114 required Mg for growth. Strain CBA1114 had three 16S rRNA genes, rrnA, rrnB and rrnC; levels of similarity between the sequences were 99.7–99.9 %. The 16S rRNA gene sequence of strain CBA1114 showed 91.7 % similarity to that of Haloterrigena thermotolerans PR5. In multilocus sequence analysis (MLSA), five housekeeping genes, atpB, EF-2, radA, rpoB′ and secY, were found to be closely related to those of the members of the genera Halorientalis (89.7 % similarity of the atpB gene sequence), Halomicroarcula (91.9 %, EF-2), Haloterrigena (85.4 %, radA), Natronoarchaeum (89.2 %, rpoB′) and Natrinema (75.7 %, secY). A phylogenetic tree generated from the results of MLSA of the five housekeeping genes showed that strain CBA1114 was closely related to species of the genus Halorientalis in the family Halobacteriaceae . The major polar lipids were identified as phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and unidentified lipids. The G+C content of the genomic DNA of strain CBA1114 was 68.1 mol%. According to the results of phylogenetic, phenotypic and chemotaxonomic analyses, we designate strain CBA1114 (=JCM 30111=KCTC 4206) as the type strain of Halostella salina gen. nov., sp. nov., a novel species of a new genus within the family Halobacteriaceae .

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2016-07-01
2019-10-23
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