1887

Abstract

Strain S3 was isolated from lagoon sediments, and appeared as transparent colonies on agar plates, with cells staining Gram-negative. Catalase and oxidase were positive. S3 hydrolyzed starch, casein and tween-20, while urea, chitin, gelatin and tween-80 were not hydrolysed. Cω6/Cω7, Cω6/Cω7,C cyclo and C were the predominant fatty acids with minor amounts of C 3-OH, C, C and C 2-OH. S3 contained diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), and phosphatidylethanolamine (PE) as major polar lipids with minor amounts of unidentified phospholipid (PL) and unidentified lipids (L1–2). Genomic DNA G+C content was 68.3 mol%. 16S rRNA gene sequence comparisons indicated that S3 represents a member of the genus in family of the class . S3 has a sequence similarity of 98.96 % with YH12, 97.93 % with CY01 and <96.97 % with other members of the genus . DNA–DNA hybridization values between S3 and the type strains of the most closely related species were clearly below the 70 % threshold. On the basis of phenotypic, genotypic and phylogenetic analysis, it is proposed that S3 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is S3 (=KEMB 563–466=JCM 31169).

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2016-07-01
2020-03-29
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