1887

Abstract

Strain S3 was isolated from lagoon sediments, and appeared as transparent colonies on agar plates, with cells staining Gram-negative. Catalase and oxidase were positive. S3 hydrolyzed starch, casein and tween-20, while urea, chitin, gelatin and tween-80 were not hydrolysed. Cω6/Cω7, Cω6/Cω7,C cyclo and C were the predominant fatty acids with minor amounts of C 3-OH, C, C and C 2-OH. S3 contained diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), and phosphatidylethanolamine (PE) as major polar lipids with minor amounts of unidentified phospholipid (PL) and unidentified lipids (L1–2). Genomic DNA G+C content was 68.3 mol%. 16S rRNA gene sequence comparisons indicated that S3 represents a member of the genus in family of the class . S3 has a sequence similarity of 98.96 % with YH12, 97.93 % with CY01 and <96.97 % with other members of the genus . DNA–DNA hybridization values between S3 and the type strains of the most closely related species were clearly below the 70 % threshold. On the basis of phenotypic, genotypic and phylogenetic analysis, it is proposed that S3 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is S3 (=KEMB 563–466=JCM 31169).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001115
2016-07-01
2021-10-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/7/2735.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001115&mimeType=html&fmt=ahah

References

  1. Cappuccino J. G., Sherman N. 1998 Microbiology – A Laboratory Manual, 5th edn. California: Benjamin/Cummings Science Publishing;
    [Google Scholar]
  2. Chang Y. H., Han J. I., Chun J., Lee K. C., Rhee M. S., Kim Y. B., Bae K. S. 2002; Comamonas koreensis sp. nov., a non-motile species from wetland in Woopo, Korea. Int J Syst Evol Microbiol 52:377–381 [View Article][PubMed]
    [Google Scholar]
  3. Chipirom K., Tanasupawat S., Akaracharanya A., Leepepatpiboon N., Prange A., Kim K. W., Chul Lee K., Lee J. S. 2012; Comamonas terrae sp. nov., an arsenite-oxidizing bacterium isolated from agricultural soil in Thailand. J Gen Appl Microbiol 58:245–251 [View Article][PubMed]
    [Google Scholar]
  4. Chou J. H., Sheu S. Y., Lin K. Y., Chen W. M., Arun A. B., Young C. C. 2007; Comamonas odontotermitis sp. nov., isolated from the gut of the termite Odontotermes formosanus . Int J Syst Evol Microbiol 57:887–891 [View Article][PubMed]
    [Google Scholar]
  5. Etchebehere C., Errazquin M. I., Dabert P., Moletta R., Muxí L. 2001; Comamonas nitrativorans sp. nov., a novel denitrifier isolated from a denitrifying reactor treating landfill leachate. Int J Syst Evol Microbiol 51:977–983 [View Article][PubMed]
    [Google Scholar]
  6. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid–deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Evol Microbiol 39:224–229
    [Google Scholar]
  7. Gumaelius L., Magnusson G., Pettersson B., Dalhammar G. 2001; Comamonas denitrificans sp. nov., an efficient denitrifying bacterium isolated from activated sludge. Int J Syst Evol Microbiol 51:999–1006 [View Article][PubMed]
    [Google Scholar]
  8. Kim K. H., Ten L. N., Liu Q. M., Im W. T., Lee S. T. 2008; Comamonas granuli sp. nov., isolated from granules used in a wastewater treatment plant. J Microbiol 46:390–395 [View Article][PubMed]
    [Google Scholar]
  9. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  10. Kim D., Lee S. S. 2014; Comamonas faecalis sp. nov., isolated from domestic pig feces. Curr Microbiol 69:102–107 [View Article][PubMed]
    [Google Scholar]
  11. Kim H. S., Srinivasan S., Lee S. S. 2016; Methyloterrigena soli gen. nov., sp. nov., a methanol-utilizing bacterium isolated from chloroethylene-contaminated soil. Int J Syst Evol Microbiol 66:101–106 [View Article][PubMed]
    [Google Scholar]
  12. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120[PubMed] [CrossRef]
    [Google Scholar]
  13. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  14. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  15. Subhash Y., Sasikala C., Ramana C. V. 2013a; Flavobacterium aquaticum sp. nov., isolated from a water sample of a rice field. Int J Syst Evol Microbiol 63:3463–3469 [CrossRef]
    [Google Scholar]
  16. Subhash Y., Tushar L., Sasikala C., Ramana C. V. 2013b; Falsirhodobacter halotolerans gen. nov., sp. nov., isolated from dry soils of a solar saltern. Int J Syst Evol Microbiol 63:2132–2137 [CrossRef]
    [Google Scholar]
  17. Subhash Y., Tushar L., Sasikala C., Ramana C. V. 2013c; Erythrobacter odishensis sp. nov. and Pontibacter odishensis sp. nov. isolated from dry soil of a solar saltern. Int J Syst Evol Microbiol 63:4524–4532 [CrossRef]
    [Google Scholar]
  18. Sun L. N., Zhang J., Chen Q., He J., Li Q. F., Li S. P. 2013; Comamonas jiangduensis sp. nov., a biosurfactant-producing bacterium isolated from agricultural soil. Int J Syst Evol Microbiol 63:2168–2173 [View Article][PubMed]
    [Google Scholar]
  19. Tago Y., Yokota A. 2004; Comamonas badia sp. nov., a floc-forming bacterium isolated from activated sludge. J Gen Appl Microbiol 50:243–248[PubMed] [CrossRef]
    [Google Scholar]
  20. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013; mega6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Bio Evol 30:2725–2729 [CrossRef]
    [Google Scholar]
  21. Xu M., Xin Y., Yu Y., Zhang J., Zhou Y., Liu H., Tian J., Li Y. 2010; Erythrobacter nanhaisediminis sp. nov., isolated from marine sediment of the South China Sea. Int J Syst Evol Microbiol 60:2215–2220 [View Article][PubMed]
    [Google Scholar]
  22. Young C. C., Chou J. H., Arun A. B., Yen W. S., Sheu S. Y., Shen F. T., Lai W. A., Rekha P. D., Chen W. M. 2008; Comamonas composti sp. nov., isolated from food waste compost. Int J Syst Evol Microbiol 58:251–256 [View Article][PubMed]
    [Google Scholar]
  23. Yu X. Y., Li Y. F., Zheng J. W., Li Y., Li L., He J., Li S. P. 2011; Comamonas zonglianii sp. nov., isolated from phenol-contaminated soil. Int J Syst Evol Microbiol 61:255–258 [View Article][PubMed]
    [Google Scholar]
  24. Zhang J., Wang Y., Zhou S., Wu C., He J., Li F. 2013; Comamonas guangdongensis sp. nov., isolated from subterranean forest sediment, and emended description of the genus Comamonas . Int J Syst Evol Microbiol 63:809–814 [View Article][PubMed]
    [Google Scholar]
  25. Zhu D., Xie C., Huang Y., Sun J., Zhang W. 2014; Description of Comamonas serinivorans sp. nov., isolated from wheat straw compost. Int J Syst Evol Microbiol 64:4141–4146 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001115
Loading
/content/journal/ijsem/10.1099/ijsem.0.001115
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error