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Abstract

A novel actinobacterial strain, designated ACD12, was isolated from a Saharan soil sample collected from Adrar province, southern Algeria. A polyphasic study was carried out to establish the taxonomic position of this strain. Strain ACD12 was observed to form extensively branched substrate mycelia. Aerial mycelium was absent or was weakly produced on all media tested, while spore chains were short with a hooked and irregular spiral form (2–3 turns). The dominant diaminopimelic acid isomer in the cell wall was -diaminopimelic acid. Glucose, ribose, galactose, mannose and madurose occured in whole-cell hydrolysates. The major phospholipid was diphosphatidylglycerol and phosphatidylinositol. The predominant menaquinone was MK-9(H). The fatty acid profile was characterized by the presence of C, C, C, CC 9 and iso-C. Results of 16S rRNA gene sequence comparisons revealed that strain ACD12shared the highest degree of 16S rRNA gene sequence similarity with DSM 45233 (98.3 %) and DSM 45043(97.8 %). All tree-making algorithms used also supported strain ACD12forming a distinct clade with its most closely related species. In addition, DNA–DNA hybridization indicated only 39.8 % relatedness with DSM 45233 and 18.7 % relatedness with DSM 45043. The combined phenotypic and genotypic data show that the novel isolate represents a novel species of the genus , for which the name sp. nov., is proposed, with the type strain ACD12 (=DSM 46745 =CECT 8842).

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2016-07-01
2021-08-01
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References

  1. Aouiche A., Bouras N., Mokrane S., Zitouni A., Schumann P., Spröer C., Sabaou N., Klenk H. P. 2015; Actinokineospora mzabensis sp. nov., a novel actinomycete isolated from Saharan soil. Antonie Van Leeuwenhoek 107:291–296 [View Article][PubMed]
    [Google Scholar]
  2. Ara I., Matsumoto A., Bakir M. A., Kudo T., Omura S., Takahashi Y. 2008; Actinomadura bangladeshensis sp. nov. and Actinomadura chokoriensis sp. nov. Int J Syst Evol Microbiol 58:1653–1659 [View Article][PubMed]
    [Google Scholar]
  3. Becker B., Lechevalier M. P., Gordon R. E., Lechevalier H. A. 1964; Rapid differentiation between Nocardia and Streptomyces by paper chromatography of whole-cell hydrolysates. Appl Microbiol 12:421–423[PubMed]
    [Google Scholar]
  4. Boubetra D., Zitouni A., Bouras N., Schumann P., Spröer C., Klenk H. P., Sabaou N. 2015; Saccharothrix tamanrassetensis sp. nov., an actinomycete isolated from Saharan soil. Int J Syst Evol Microbiol 65:1316–1320 [View Article][PubMed]
    [Google Scholar]
  5. Bouras N., Meklat A., Zitouni A., Mathieu F., Schumann P., Spröer C., Sabaou N., Klenk H. P. 2015; Nocardiopsis algeriensis sp. nov., an alkalitolerant actinomycete isolated from Saharan soil. Antonie Van Leeuwenhoek 107:313–320 [View Article][PubMed]
    [Google Scholar]
  6. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [View Article][PubMed]
    [Google Scholar]
  7. Cook A. E., Roes M., Meyers P. R. 2005; Actinomadura napierensis sp. nov., isolated from soil in South Africa. Int J Syst Evol Microbiol 55:703–706 [View Article][PubMed]
    [Google Scholar]
  8. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [View Article][PubMed]
    [Google Scholar]
  9. Euanorasetr J., Intra B., Mongkol P., Chankhamhaengdecha S., Tuchinda P., Mori M., Shiomi K., Nihira T., Panbangred W. 2015; Spirotetronate antibiotics with anti-Clostridium activity from Actinomadura sp. 2EPS. World J Microbiol Biotechnol 31:391–398 [View Article][PubMed]
    [Google Scholar]
  10. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
    [Google Scholar]
  11. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  12. Fitch W. M. 1977; On the Problem of discovering the most parsimonious tree. Am Nat 111:223–257 [View Article]
    [Google Scholar]
  13. Goodfellow M. 1971; Numerical taxonomy of some nocardioform bacteria. J Gen Microbiol 69:33–90 [View Article][PubMed]
    [Google Scholar]
  14. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H. N. 1974; Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J of Bacteriol 24:54–63 [View Article]
    [Google Scholar]
  15. Hayakawa M., Nonomura H. 1987; Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. J Ferm Technol 65:501–509 [View Article]
    [Google Scholar]
  16. Huss V. A., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [View Article][PubMed]
    [Google Scholar]
  17. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp 21–132 Edited by Munro H. N. New York: Academic. Press; [CrossRef]
    [Google Scholar]
  18. Kelly K. L., Judd D. B. 1976 Color: Universal Language and Dictionary of Names (National Bureau of Standards Special Publication 440) Washington, DC: US Department of Commerce; [CrossRef]
    [Google Scholar]
  19. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  20. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  21. Kroppenstedt R. M. 1982; Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a Silver loaded ion exchanger as stationary Phases. J Liq Chromatogr 5:2359–2367 [View Article]
    [Google Scholar]
  22. Kroppenstedt R. M. 1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series) vol. 20 pp 173–179 Edited by Goodfellow. M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  23. Kroppenstedt R. M., Stackebrandt E., Goodfellow M. 1990; Taxonomic revision of the actinomycete genera Actinomadura and Microtetraspora . Syst Appl Microbiol 13:148–160 [View Article]
    [Google Scholar]
  24. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A. et al. 2007; clustal w and clustal x version 2.0. Bioinformatics 23:2947–2948 [View Article][PubMed]
    [Google Scholar]
  25. Lechevalier H. A., Lechevalier M. P. 1968; A critical evaluation of the genera of aerobic actinomycetes. In The Actinomycetales pp 393–405 Edited by Prauser H. Jena: VEB Gustav Fischer Verlag;
    [Google Scholar]
  26. Lechevalier M. P., Lechevalier H. 1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443 [View Article]
    [Google Scholar]
  27. Lechevalier M. P., De Bievre C., Lechevalier H. 1977; Chemotaxonomy of aerobic Actinomycetes: Phospholipid composition. Biochem Syst Ecol 5:249–260 [View Article]
    [Google Scholar]
  28. Lee S. D., Jeong H. S. 2006; Actinomadura hallensis sp. nov., a novel actinomycete isolated from Mt. Halla in Korea. Int J Syst Evol Microbiol 56:259–264 [View Article][PubMed]
    [Google Scholar]
  29. Lu Z., Wang L., Zhang Y., Shi Y., Liu Z., Quintana E. T., Goodfellow M. 2003; Actinomadura catellatispora sp. nov. and Actinomadura glauciflava sp. nov., from a sewage ditch and soil in southern China. Int J Syst Evol Microbiol 53:137–142 [View Article][PubMed]
    [Google Scholar]
  30. Marchal N., Bourdon J. L., Richard C. L. 1987 Les Milieux De Culture Pour L’isolement Et L’identification Biochimique Des Bactéries Paris: Doin Press;
    [Google Scholar]
  31. Meklat A., Bouras N., Mokrane S., Zitouni A., Schumann P., Spröer C., Klenk H. P., Sabaou N. 2015; Bounagaea algeriensis gen. nov., sp. nov., an extremely halophilic actinobacterium isolated from a Saharan soil of Algeria. Antonie Van Leeuwenhoek 108:473–482 [View Article][PubMed]
    [Google Scholar]
  32. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
    [Google Scholar]
  33. Quintana E. T., Trujillo M. E., Goodfellow M. 2003; Actinomadura mexicana sp. nov. and Actinomadura meyerii sp. nov., two novel soil sporoactinomycetes. Syst Appl Microbiol 26:511–517 [View Article][PubMed]
    [Google Scholar]
  34. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46:1088–1092 [View Article][PubMed]
    [Google Scholar]
  35. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  36. Saker R., Bouras N., Zitouni A., Ghoul M., Rohde M., Schumann P., Spröer C., Sabaou N., Klenk H. P. 2014; Mzabimyces algeriensis gen. nov., sp. nov., a halophilic filamentous actinobacterium isolated from a Saharan soil, and proposal of Mzabimycetaceae fam. nov. Antonie Van Leeuwenhoek 106:1021–1030 [View Article][PubMed]
    [Google Scholar]
  37. Sasser M. 1990; Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids. MIDI Technical Note 101 Newark DE: MIDI Inc;
    [Google Scholar]
  38. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [View Article]
    [Google Scholar]
  39. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011; MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol and Evol 28:2731–2739 [View Article]
    [Google Scholar]
  40. Waksman S. A. 1961 The Actinomycetes, Classification, Identification, Descriptions of Genera and Species vol. 2 Baltimore: Williams & Wilkins;
    [Google Scholar]
  41. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
    [Google Scholar]
  42. Wink J., Kroppenstedt R. M., Seibert G., Stackebrandt E. 2003; Actinomadura namibiensis sp. nov. Int J Syst Evol Microbiol 53:721–724 [View Article][PubMed]
    [Google Scholar]
  43. Yassin A. F., Spröer C., Siering C., Klenk H. P. 2010; Actinomadura sputi sp. nov., isolated from the sputum of a patient with pulmonary infection. Int J Syst Evol Microbiol 60:149–153 [View Article][PubMed]
    [Google Scholar]
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