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Abstract

A novel bacterium, designated KJ031, was isolated from a wetland soil sample taken from Jeju island, Republic of Korea. Cells were Gram-stain-negative, curved rod shaped, oxidase- and catalase- positive, motile and facultatively anaerobic. Growth was observed at pH 6.0–8.0 and at 20–37 °C on R2A agar. Comparative analysis of 16S rRNA gene sequences revealed that strain KJ031 is a member of the genus Paludibacterium , sharing highest sequence similarities with Paludibacterium paludis KBP-21 (96.2 %) and Paludibacterium. yongneupense 5YN8-15 (96.0 %). The major fatty acids were summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c), C16 : 0 and summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c). The predominant respiratory quinone was Q-8. The major polar lipids of strain KJ031 were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminophospholipid, two unidentified phospholipids and one unidentified polar lipid. The DNA G+C content was 59.2 mol%. On the basis of the evidence presented in this study, strain KJ031 represents a novel species of the genus Paludibacterium , for which the name Paludibacterium purpuratum sp. nov. is proposed. The type strain is KJ031 (=KCTC 42852 =CECT 8976).

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2016-07-01
2019-10-20
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