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Abstract

A Gram-stain-negative, aerobic, non-spore-forming, motile by gliding and coccoid, ovoid or rod-shaped bacterial strain, designated GHTF-27, was isolated from a tidal flat at Goheung, South Korea, and subjected to a taxonomic study using a polyphasic approach. The novel strain grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0–5.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain GHTF-27 belongs to the genus Gramella . It exhibited 16S rRNA gene sequence similarity values of 97.6–98.3 % to the type strains of Gramella aestuariivivens , Gramella echinicola , Gramella gaetbulicola , Gramella aquimixticola , Gramella aestuarii and Gramella portivictoriae , respectively, and of 93.8–96.7 % to the type strains of the other species of the genus Gramellawith validly published names. Strain GHTF-27 contained MK-6 as the predominant menaquinone and iso-C15 : 0 and iso-C17 : 0 3-OH as the major fatty acids. The major polar lipids of strain GHTF-27 were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain GHTF-27 was 39.7 mol% and DNA–DNA relatedness values with the type strains of the six phylogenetically closely related species of the genus Gramellawere 12–28 %. The differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain GHTF-27 is separated from other species of the genus Gramella . On the basis of the data presented, strain GHTF-27 is considered to represent a novel species of the genus Gramella , for which the name Gramella sediminilitorissp. nov. is proposed. The type strain is GHTF-27 (=KCTC 52208 =NBRC 111992).

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2016-07-01
2019-10-19
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