@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001107, author = "Liu, Bo and Liu, Guo-Hong and Zhu, Yu-Jing and Wang, Jie-Ping and Che, Jian-Mei and Chen, Qian-Qian and Chen, Zheng", title = "Bacillus loiseleuriae sp. nov., isolated from rhizosphere soil from a loiseleuria plant", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2016", volume = "66", number = "7", pages = "2678-2683", doi = "https://doi.org/10.1099/ijsem.0.001107", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001107", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "rhizosphere soil", keywords = "genome", keywords = "taxono-genomics", keywords = "Bacillus loiseleuriae", abstract = "A Gram-stain-positive, rod-shaped, endospore-forming, aerobic bacterium, designated strain FJAT-27997T, was isolated from the rhizosphere soil of a Loiseleuria plant collected from Sichuan province in China. Growth was observed aerobically between 20  and 35 °C (optimum 30 °C), between 0 and 3.0 % (w/v) NaCl (optimum at 0 %) concentration and pH in the range 6.0–9.0 (optimum at pH 7.0). The cell-wall peptidoglycan contained meso-diaminopimelic acid and the major isoprenoid quinone was menaquinone MK-7. The main fatty acids were iso-C15 : 0, anteiso-C15 : 0, iso-C14 : 0, C16 : 0 and C14 : 0. The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Phylogenetic analyses based on 16S rRNA gene sequences showed that isolate FJAT-27997T was a member of the genus Bacillus and was related most closely to Bacillus simplex DSM 1321T (97.95 % similarity), followed by Bacillus huizhouensis GSS03T (97.9 %). The average nucleotide identity value between strain FJAT-27997T and the most closely related species, B . simplex DSM 1321T, was 71.60 % (JSpecies), less than the previously proposed cut-off value of 96 % for differentiating species within the genus. The in silico DNA–DNA hybridization values between strain FJAT-27997T and its most closely related species were <70 %, again indicating they belong to different taxa. The main fatty acids were iso-C15 : 0 and anteiso-C15 : 0. The novel strain could be differentiated from other known Bacillus species on the basis of several phenotypic characters and fatty acid profiles. This taxononomic/genomic study revealed that strain FJAT-27997T represents a novel Bacillus species, for which the name Bacillus loiseleuriae sp. nov. (type strain FJAT-27997T =CCTCC AB 2015285T=DSM 101776T) is proposed.", }