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Volume 66, Issue 7

sp. nov., an endophyte of maize Free

Abstract

A yellow-pigmented strain (JM-1081) isolated from healthy stem tissue of was taxonomically characterized. Cells of the strain were rod-shaped and Gram-stain-negative. Comparative 16S rRNA gene sequence analysis revealed closest relationship to the type strains of (98.1 % similarity), (97.9 %) and (97.8 %). 16S rRNA gene sequence similarities to the type strains of all other species were below 97.8 %. Fatty acid analysis of whole-cell hydrolysates of the strain resulted in a pattern typical of the genus with iso-C 2-OH and/or C 7, iso-C, iso-C 3-OH and C and as major compounds. The polyamine pattern contained predominantly -homospermidine. The major quinone was menaquinone MK-7 and the only identified lipids in the polar lipid profile were phosphatidylethanolamine and phosphatidylserine. In addition, 15 unidentified lipids were detected in moderate to major amounts. Sphingolipid was detected. The diagnostic diamino acid of the peptidoglycan was -diaminopimelic acid. DNA–DNA hybridizations with two of the closely related type strains, those of and , as well as resulted in values below 70 %. In addition to the genotypic differences, differential biochemical and chemotaxonomic properties confirmed that the isolate JM-1081represents a novel species, for which the name sp. nov. is proposed. The type strain is JM-1081 (=LMG 29191=CCM 8652).

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  • Accepted:
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Keyword(s): endophyte , plant associated , Sphingobacterium zeae and taxonomy
© 2016 IUMS
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2016-07-01
2024-04-16
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Sphingobacterium zeae sp. nov., an endophyte of maize
Int J Syst Evol Microbiol 66, 2643 (2016); https://doi.org/10.1099/ijsem.0.001100
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