1887

Abstract

Two Gram-staining-negative, aerobic, motile, slimy, glossy bacterial strains were isolated from bark tissue of Populus×euramericana. The bacteria grew at 10–37 °C, pH 5–10, with optimal growth at 28–30 °C, pH 6.0–8.0. Both strains grew with 0–3 % (w/v) NaCl. In the maximum-likelihood phylogenetic tree, the two isolates formed a distinct branch within the family Phyllobacteriaceae , and they were not closely related to any of the genera within the family Phyllobacteriaceae . The two novel isolates werepositive for oxidase andcatalase activity. The polar lipids profile revealed diphosphatidylglycerol, phosphatidylcholine, phospholipid, phosphatidylethanolamine, phosphatidylglycerol and five unknown lipids. The major fatty acids were C18 : 1ω7c and C16 : 0. The DNA G+C content was 56.4 mol%. On the basis of phylogenetic, chemotaxonomic and phenotypic data, the two strains represent a novel species belonging to a novel genus of the family Phyllobacteriaceae , for which the name Corticibacterium populi gen. nov., sp. nov. is proposed. The type strain of the type species is 16B10-2-7 (=CFCC 12884=KCTC 42249).

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2016-07-01
2019-10-22
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References

  1. Baker G. C. , Smith J. J. , Cowan D. A. . ( 2003;). Review and re-analysis of domain-specific 16S primers. . J Microbiol Methods 55: 541–555.[PubMed] [CrossRef]
    [Google Scholar]
  2. Bambauer A. , Rainey F. A. , Stackebrandt E. , Winter J. . ( 1998;). Characterization of Aquamicrobium defluvii gen. nov. sp. nov., a thiophene-2-carboxylate-metabolizing bacterium from activated sludge. . Arch Microbiol 169: 293–302.[PubMed] [CrossRef]
    [Google Scholar]
  3. Collins M. D. , Pirouz T. , Goodfellow M. , Minnikin D. E. . ( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100: 221–230. [CrossRef] [PubMed]
    [Google Scholar]
  4. De smedt J. , De ley J. . ( 1977;). Intra- and Intergeneric Similarities of Agrobacterium Ribosomal Ribonucleic Acid Cistrons. . Int J Syst Bacteriol 27: 222–240.[CrossRef]
    [Google Scholar]
  5. Du H. J. , Zhang Y. Q. , Liu H. Y. , Su J. , Wei Y. Z. , Ma B. P. , Guo B. L. , Yu L. Y. . ( 2013;). Allonocardiopsis opalescens gen. nov., sp. nov., a new member of the suborder Streptosporangineae, from the surface-sterilized fruit of a medicinal plant. . Int J Syst Evol Microbiol 63: 900–904. [CrossRef] [PubMed]
    [Google Scholar]
  6. Felsenstein J. . ( 1985;). Confidence Limits on Phylogenies: An Approach Using the Bootstrap. . Evolution 39: 783–791.[CrossRef]
    [Google Scholar]
  7. Gerhardt P. , Murray R. G. E. , Costilow R. N. , Nester E. W. , Wood W. A. , Krieg N. R. , Phillips G. R. . ( 1981;). Manual of Methods for General Bacteriology, Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  8. Groth I. , Schumann P. , Rainey F. A. , Martin K. , Schuetze B. , Augsten K. . ( 1997;). Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. . Int J Syst Bacteriol 47: 1129–1133. [CrossRef] [PubMed]
    [Google Scholar]
  9. Hameed A. , Shahina M. , Lai W. A. , Lin S. Y. , Young L. S. , Liu Y. C. , Hsu Y. H. , Young C. C. . ( 2015;). Oricola cellulosilytica gen. nov., sp. nov., a cellulose-degrading bacterium of the family Phyllobacteriaceae isolated from surface seashore water, and emended descriptions of Mesorhizobium loti and Phyllobacterium myrsinacearum . . Antonie Van Leeuwenhoek 107: 759–771. [CrossRef] [PubMed]
    [Google Scholar]
  10. Jarvis B. D. W. , Van Berkum P. , Chen W. X. , Nour S. M. , Fernandez M. P. , Cleyet-Marel J. C. , Gillis M. . ( 1997;). Transfer of Rhizobium loti, Rhizobium huakuii, Rhizobium ciceri, Rhizobium mediterraneum, and Rhizobium tianshanense to Mesorhizobium gen. nov. . Int J Syst Bacteriol 47: 895–898.[CrossRef]
    [Google Scholar]
  11. Jenkins D. , Richard M. G. , Daigger G. T. . ( 1986;). Manual on the Causes and Control of Activated Sludge Bulking and Foaming, Water Research Commission;.
    [Google Scholar]
  12. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. et al. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62: 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  13. Kämpfer P. , Müller C. , Mau M. , Neef A. , Auling G. , Busse H. J. , Osborn A. M. , Stolz A. . ( 1999;). Description of Pseudaminobacter gen. nov. with two new species, Pseudaminobacter salicylatoxidans sp. nov. and Pseudaminobacter defluvii sp. nov. . Int J Syst Bacteriol 49: 887–897. [CrossRef] [PubMed]
    [Google Scholar]
  14. Lane D. J. . ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt. E. , Goodfellow M. . Chichester:: Wiley;.
    [Google Scholar]
  15. Li Y. , He W. , Wang T. , Piao C. G. , Guo L. M. , Chang J. P. , Guo M. W. , Xie S. J. . ( 2014;). Acinetobacter qingfengensis sp. nov., isolated from canker bark of Populus x euramericana . . Int J Syst Evol Microbiol 64: 1043–1050. [CrossRef] [PubMed]
    [Google Scholar]
  16. Lipski A. , Kämpfer P. . ( 2012;). Aquamicrobium ahrensii sp. nov and Aquamicrobium segne sp. nov. From Experimental Biofilters . . Int J Syst Evol Microbiol 62: 2511–2516.[CrossRef]
    [Google Scholar]
  17. Marmur J. . ( 1961;). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. . J MolBiol 3: 208–218. [CrossRef]
    [Google Scholar]
  18. Mergaert J. , Swings J. . ( 2005;). Family IV. Phyllobacteriaceae fam. nov. Bergey’s Manual of Systematic Bacteriology . . In (The Proteobacteria), Part C (The Alpha-, Beta-, Delta-, and Epsilonproteobacteria, , 2nd edn.,vol. 2 393 Edited by Brenner D. J. , Krieg N. R. , Staley. J. T. , Garrity G. M. . New York:: Springer;.
    [Google Scholar]
  19. Mergaert J. , Cnockaert M. C. , Swings J. . ( 2002;). Phyllobacterium myrsinacearum (subjective synonym Phyllobacterium rubiacearum) emend. . Int J Syst Evol Microbiol 52: 1821–1823. [CrossRef] [PubMed]
    [Google Scholar]
  20. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989;). Precise Measurement of the G+C Content of Deoxyribonucleic Acid by High-Performance Liquid Chromatography. . International Journal of Systematic Bacteriology 39: 159–167.[CrossRef]
    [Google Scholar]
  21. Minnikin D. E. , O'Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . Journal of Microbiological Methods 2: 233–241.[CrossRef]
    [Google Scholar]
  22. Sasser M. . ( 1990;). Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI. . Technical Notes 101, Newark, DE:: MIDI Inc;.
    [Google Scholar]
  23. Smibert R. M. , Krieg N. R. . ( 1994;). Phenotypic characterization. . In Manual of Methods for General and Microbiology, pp. 607–654. Edited by Gerhardt P. , Murray R. G. E. , Wood. W. A. , Krieg M N. R. . Washington, D C:: American Society for Microbiology;.
    [Google Scholar]
  24. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011;). MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28: 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  25. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997;). The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25: 4876–4882.[PubMed] [CrossRef]
    [Google Scholar]
  26. Urakami T. , Araki H. , Oyanagi H. , Suzuki K.-I. , Komagata K. . ( 1992;). Transfer of Pseudomonas aminovorans (den Dooren de Jong 1926) to Aminobacter gen. nov. as Aminobacter aminovorans comb. nov. and Description of Aminobacter aganoensis sp. nov. and Aminobacter niigataensis sp. nov. . Int J Syst Bacteriol 42: 84–92. [CrossRef]
    [Google Scholar]
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