1887

Abstract

Two Gram-staining-negative, aerobic, motile, slimy, glossy bacterial strains were isolated from bark tissue of ×. The bacteria grew at 10–37 °C, pH 5–10, with optimal growth at 28–30 °C, pH 6.0–8.0. Both strains grew with 0–3 % (w/v) NaCl. In the maximum-likelihood phylogenetic tree, the two isolates formed a distinct branch within the family , and they were not closely related to any of the genera within the family . The two novel isolates werepositive for oxidase andcatalase activity. The polar lipids profile revealed diphosphatidylglycerol, phosphatidylcholine, phospholipid, phosphatidylethanolamine, phosphatidylglycerol and five unknown lipids. The major fatty acids were Cω7 and C. The DNA G+C content was 56.4 mol%. On the basis of phylogenetic, chemotaxonomic and phenotypic data, the two strains represent a novel species belonging to a novel genus of the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is 16B10-2-7 (=CFCC 12884=KCTC 42249).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001097
2016-07-01
2020-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/7/2617.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001097&mimeType=html&fmt=ahah

References

  1. Baker G. C., Smith J. J., Cowan D. A.. 2003; Review and re-analysis of domain-specific 16S primers. J Microbiol Methods55:541–555[PubMed][CrossRef]
    [Google Scholar]
  2. Bambauer A., Rainey F. A., Stackebrandt E., Winter J.. 1998; Characterization of Aquamicrobium defluvii gen. nov. sp. nov., a thiophene-2-carboxylate-metabolizing bacterium from activated sludge. Arch Microbiol169:293–302[PubMed][CrossRef]
    [Google Scholar]
  3. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E.. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol100:221–230 [CrossRef][PubMed]
    [Google Scholar]
  4. De smedt J., De ley J.. 1977; Intra- and Intergeneric Similarities of Agrobacterium Ribosomal Ribonucleic Acid Cistrons. Int J Syst Bacteriol27:222–240[CrossRef]
    [Google Scholar]
  5. Du H. J., Zhang Y. Q., Liu H. Y., Su J., Wei Y. Z., Ma B. P., Guo B. L., Yu L. Y.. 2013; Allonocardiopsis opalescens gen. nov., sp. nov., a new member of the suborder Streptosporangineae, from the surface-sterilized fruit of a medicinal plant. Int J Syst Evol Microbiol63:900–904 [CrossRef][PubMed]
    [Google Scholar]
  6. Felsenstein J.. 1985; Confidence Limits on Phylogenies: An Approach Using the Bootstrap. Evolution39:783–791[CrossRef]
    [Google Scholar]
  7. Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. R.. 1981; Manual of Methods for General Bacteriology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  8. Groth I., Schumann P., Rainey F. A., Martin K., Schuetze B., Augsten K.. 1997; Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol47:1129–1133 [CrossRef][PubMed]
    [Google Scholar]
  9. Hameed A., Shahina M., Lai W. A., Lin S. Y., Young L. S., Liu Y. C., Hsu Y. H., Young C. C.. 2015; Oricola cellulosilytica gen. nov., sp. nov., a cellulose-degrading bacterium of the family Phyllobacteriaceae isolated from surface seashore water, and emended descriptions of Mesorhizobium loti and Phyllobacterium myrsinacearum . Antonie Van Leeuwenhoek107:759–771 [CrossRef][PubMed]
    [Google Scholar]
  10. Jarvis B. D. W., Van Berkum P., Chen W. X., Nour S. M., Fernandez M. P., Cleyet-Marel J. C., Gillis M.. 1997; Transfer of Rhizobium loti, Rhizobium huakuii, Rhizobium ciceri, Rhizobium mediterraneum, and Rhizobium tianshanense to Mesorhizobium gen. nov. Int J Syst Bacteriol47:895–898[CrossRef]
    [Google Scholar]
  11. Jenkins D., Richard M. G., Daigger G. T.. 1986; Manual on the Causes and Control of Activated Sludge Bulking and Foaming Water Research Commission;
    [Google Scholar]
  12. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  13. Kämpfer P., Müller C., Mau M., Neef A., Auling G., Busse H. J., Osborn A. M., Stolz A.. 1999; Description of Pseudaminobacter gen. nov. with two new species, Pseudaminobacter salicylatoxidans sp. nov. and Pseudaminobacter defluvii sp. nov. Int J Syst Bacteriol49:887–897 [CrossRef][PubMed]
    [Google Scholar]
  14. Lane D. J.. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp115–175 Edited by Stackebrandt. E., Goodfellow M.. Chichester: Wiley;
    [Google Scholar]
  15. Li Y., He W., Wang T., Piao C. G., Guo L. M., Chang J. P., Guo M. W., Xie S. J.. 2014; Acinetobacter qingfengensis sp. nov., isolated from canker bark of Populus x euramericana . Int J Syst Evol Microbiol64:1043–1050 [CrossRef][PubMed]
    [Google Scholar]
  16. Lipski A., Kämpfer P.. 2012; Aquamicrobium ahrensii sp. nov and Aquamicrobium segne sp. nov. From Experimental Biofilters . Int J Syst Evol Microbiol62:2511–2516[CrossRef]
    [Google Scholar]
  17. Marmur J.. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J MolBiol3:208–218 [CrossRef]
    [Google Scholar]
  18. Mergaert J., Swings J.. 2005; Family IV. Phyllobacteriaceae fam. nov. Bergey’s Manual of Systematic Bacteriology . In (The Proteobacteria), Part C (The Alpha-, Beta-, Delta-, and Epsilonproteobacteria, 2nd edn.vol. 2393 Edited by Brenner D. J., Krieg N. R., Staley. J. T., Garrity G. M.. New York: Springer;
    [Google Scholar]
  19. Mergaert J., Cnockaert M. C., Swings J.. 2002; Phyllobacterium myrsinacearum (subjective synonym Phyllobacterium rubiacearum) emend. Int J Syst Evol Microbiol52:1821–1823 [CrossRef][PubMed]
    [Google Scholar]
  20. Mesbah M., Premachandran U., Whitman W. B.. 1989; Precise Measurement of the G+C Content of Deoxyribonucleic Acid by High-Performance Liquid Chromatography. International Journal of Systematic Bacteriology39:159–167[CrossRef]
    [Google Scholar]
  21. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. Journal of Microbiological Methods2:233–241[CrossRef]
    [Google Scholar]
  22. Sasser M.. 1990; Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI. Technical Notes 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  23. Smibert R. M., Krieg N. R.. 1994; Phenotypic characterization. In Manual of Methods for General and Microbiology pp607–654 Edited by Gerhardt P., Murray R. G. E., Wood. W. A., Krieg M N. R.. Washington, D C: American Society for Microbiology;
    [Google Scholar]
  24. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. 2011; MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol28:2731–2739 [CrossRef][PubMed]
    [Google Scholar]
  25. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. 1997; The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res25:4876–4882[PubMed][CrossRef]
    [Google Scholar]
  26. Urakami T., Araki H., Oyanagi H., Suzuki K.-I., Komagata K.. 1992; Transfer of Pseudomonas aminovorans (den Dooren de Jong 1926) to Aminobacter gen. nov. as Aminobacter aminovorans comb. nov. and Description of Aminobacter aganoensis sp. nov. and Aminobacter niigataensis sp. nov. Int J Syst Bacteriol42:84–92 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001097
Loading
/content/journal/ijsem/10.1099/ijsem.0.001097
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited this month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error