1887

Abstract

Four strains alternating between yeast and filamentous growth morphologies were isolated from flowers in two regions of Laos. In liquid environment the isolates propagated by budding and developed irregularly shaped pseudohyphae. On solid media, their yeast cells switched to hyphal growth which could return to the yeast phase by developing lateral blastoconidia. The sequences of the D1/D2 domains of the large subunit (LSU) 26S rRNA genes, the internal transcribed spacer (ITS) regions and the small subunit (SSU) 18S rRNA genes were identical in the four strains and differed from the corresponding sequences of other yeast species available in databases by at least 11 % (D1/D2), 13 % (ITS) and 7 % (SSU). In an independent project, two strains with D1/D2 and ITS sequences very similar to those of the Laotian strains were found in bark samples collected in Brazil. The six strains also differed from the closest yeast species in physiological properties, indicating that they represented a hitherto undescribed species. Phylogenetic analysis of the D1/D2 sequences, and the concatenated sequences of the SSU rRNA genes, D1/D2 domains of LSU rRNA genes as well as the protein-encoding genes ACT1 and TEF1 placed thestrains close to Hyphopichia. To reflect this position, the novel genus name Metahyphopichia gen. nov. and the novel species name Metahyphopichia laotica gen. nov., sp. nov. are proposed for them. The type strain of the type species is 11-1006(=CBS 13022=CCY 092-001-001=NCAIM Y.02126) and was isolated in Luang Prabang (Laos). MycoBank registration numbers are MB 808253 (Metahyphopichia) and MB 808254 (Metahyphopichia laotica).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001091
2016-07-01
2019-12-07
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/7/2550.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001091&mimeType=html&fmt=ahah

References

  1. Barnett J. A. , Payne R. W. , Yarroa D. . ( 1990;). In Yeasts: Characteristics and Identification, , 2nd Edn.. Cambridge, UK:: Cambridge University Press;.
    [Google Scholar]
  2. Cendejas-Bueno E. , Kolecka A. , Alastruey-Izquierdo A. , Theelen B. , Groenewald M. , Kostrzewa M. , Cuenca-Estrella M. , Gómez-López A. , Boekhout T. . ( 2012;). Reclassification of the Candida haemulonii complex as Candida haemulonii (C. haemulonii group I), C. duobushaemulonii sp. nov. (C. haemulonii group II), and C. haemulonii var. vulnera var. nov.: three multiresistant human pathogenic yeasts. . J Clin Microbiol 50: 3641–3651. [CrossRef] [PubMed]
    [Google Scholar]
  3. Chen Y.-C. , Eisner J. D. , Kattar M. M. , Rassoulian-Barrett S. L. , Lafe K. , Bui U. , Limaye A. P. , Cookson B. T. . ( 2001;). Polymorphic internal transcribed spacer region 1 DNA sequences identify medically important yeasts. . J Clin Microbiol 39: 4042–4051. [CrossRef] [PubMed]
    [Google Scholar]
  4. Felsenstein J. , Churchill G. A. . ( 1996;). A Hidden Markov Model approach to variation among sites in rate of evolution. . Mol Biol Evol 13: 93–104. [CrossRef] [PubMed]
    [Google Scholar]
  5. Felsenstein J. . ( 2007;). PHYLIP (phylogeny inference package), version 3.67. Distributed by the author. . Department of Genome Sciences, University of Washington, Seattle, USA;.
  6. Freitas L. F. , Carvajal Barriga E. J. , Barahona P. P. , Lachance M.-A. , Rosa C. A. . ( 2013;). Kodamaea transpacifica f.a., sp. nov., a yeast species isolated from ephemeral flowers and insects in the Galapagos Islands and Malaysia: futher evidence for ancient human transpacific contacts. . Int J Syst Evol Microbiol 63: 4324–4329. [CrossRef]
    [Google Scholar]
  7. Gancedo J. M. . ( 2001;). Control of pseudohyphae formation in Saccharomyces cerevisiae . . FEMS Microbiol Rev 25: 107–123. [CrossRef] [PubMed]
    [Google Scholar]
  8. Groenewald M. , Smith M. T. . ( 2010;). Re-examination of strains formerly assigned to Hyphopichia burtonii, the phylogeny of the genus Hyphopichia, and the description of Hyphopichia pseudoburtonii sp. nov. . Int J Syst Evol Microbiol 60: 2675–2680. [CrossRef] [PubMed]
    [Google Scholar]
  9. Guindon S. , Dufayard J. F. , Lefort V. , Anisimova M. , Hordijk W. , Gascuel O. . ( 2010;). New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of phyML 3.0. . Systematic Biology 59: 307–321. [CrossRef]
    [Google Scholar]
  10. Han T.-L. , Cannon R. D. , Villas-Bôas S. G. . ( 2011;). The metabolic basis of Candida albicans morphogenesis and quorum sensing. . Fungal Genet Biol 48: 747–763. [CrossRef] [PubMed]
    [Google Scholar]
  11. Kann V. L. . ( 1993;). Polymerase chain reaction for the diagnosis of candidemia. . J Infect Dis 168: 779–783. [CrossRef] [PubMed]
    [Google Scholar]
  12. Katoh K. , Toh H. . ( 2008;). Recent developments in the MAFFT multiple sequence alignment program. . Brief Bioinform 9: 286–298. [CrossRef] [PubMed]
    [Google Scholar]
  13. Kurtzman C. P. , Robnett C. J. . ( 2003;). Phylogenetic relationships among yeasts of the 'Saccharomyces complex' determined from multigene sequence analyses. . FEMS Yeast Res 3: 417–432. [CrossRef] [PubMed]
    [Google Scholar]
  14. Kurtzman C. P. , Fell J. W. , Boekhout T. . ( 2011;). The yeasts: a taxonomic study. Amsterdam:: Elsevier;.
    [Google Scholar]
  15. Kurtzman C. P. . ( 2011a;). Hyphopichia von Arx & van der Walt (1976). . In The Yeasts: a Taxonomic Study, , 5th edn.,vol. 1 , pp. 435–438. Edited by Kurtzman C. P. , Fell. J. W. , Boekhout T. . Amsterdam:: Elsevier;.[CrossRef]
    [Google Scholar]
  16. Kurtzman C. P. . ( 2011b;). Phylogeny of the ascomycetous yeasts and the renaming of Pichia anomala to Wickerhamomyces anomalus . . Antonie Van Leeuwenhoek 99: 13–23. [CrossRef]
    [Google Scholar]
  17. Kurtzman C. P. . ( 2012;). Citeromyces hawaiiensis sp. nov., an ascosporic yeast associated with Myoporum sandwicense . . Int J Syst Evol Microbiol 62: 1215–1219. [CrossRef] [PubMed]
    [Google Scholar]
  18. Kurtzman C. P. , Robnett C. J. . ( 2014;). Three new anascosporic genera of the Saccharomycotina: Danielozyma gen. nov., Deakozyma gen. nov. and Middelhovenomyces gen. nov. . Antonie Van Leeuwenhoek 105: 933–942. [CrossRef] [PubMed]
    [Google Scholar]
  19. Lachance M. A. , Rosa C. A. , Carvajal E. J. , Freitas L. F. , Bowles J. M. . ( 2012;). Saccharomycopsis fodiens sp. nov., a rare predacious yeast from three distant localities. . Int J Syst Evol Microbiol 62: 2793–2798. [CrossRef] [PubMed]
    [Google Scholar]
  20. Lachance M.-A. . ( 2011;). Metschnikowia Kamienski. . In The Yeasts: a Taxonomic Study, , 5th edn.,vol. 1 , pp. 575–620. Edited by Kurtzman C. P. , Fell. J. W. , Boekhout T. . Amsterdam:: Elsevier;.[CrossRef]
    [Google Scholar]
  21. Limtong S. , Kaewwichian R. , Jindamorakot S. , Yongmanitchai W. , Nakase T. . ( 2012;). Candida wangnamkhiaoensis sp. nov., an anamorphic yeast species in the Hyphopichia clade isolated in Thailand. . Antonie Van Leeuwenhoek 102: 23–28. [CrossRef] [PubMed]
    [Google Scholar]
  22. Nagy L. G. , Ohm R. A. , Kovacs G. M. , Floudas D. , Riley R. , Gacser A. , Sipiczki M. , Davis J. M. , Doty S. L. et al. ( 2014;). Latent homology and convergent regulatory evolution underlies the repeated emergence of yeasts. . Nat Commun 5: 4471.[CrossRef]
    [Google Scholar]
  23. Nemecek J. C. , Wüthrich M. , Klein B. S. . ( 2006;). Global control of dimorphism and virulence in fungi. . Science 312: 583–588. [CrossRef] [PubMed]
    [Google Scholar]
  24. Nilsson R. H. , Kristiansson E. , Ryberg M. , Hallenberg N. , Larsson K.-H. . ( 2008;). Intraspecific ITS variability in the Kingdom Fungi as expressed in the international sequence databases and its implications for molecular species identification. . Evolutionary Bioinformatics 4: 193–201.
    [Google Scholar]
  25. O’Donnell K. . ( 1993;). Fusarium and its near relatives. . In The Fungal Holomorph: Mitotic, Meiotic and Pleomorphic Speciation in Fungal Systematics , pp. 225–233. Edited by Reynolds. D. R. , Taylor J. W. . Wallingford, UK:: CAB International;.
    [Google Scholar]
  26. Page R. D. M. . ( 1996;). TreeView: an application to display phylogenetic trees on personal computers. . Comput Appl Biosci 12: 357–358.[PubMed]
    [Google Scholar]
  27. Posada D. . ( 2008;). jModelTest: phylogenetic model averaging. . Mol Biol Evol 25: 1253–1256. [CrossRef] [PubMed]
    [Google Scholar]
  28. Ronquist F. , Teslenko M. , van der Mark P. , Ayres D. L. , Darling A. , Hohna S. , Larget B. , Liu L. , Suchard M. A. , Huelsenbeck J. P. . ( 2012;). MrBayes 3.2: Efficient Bayesian Phylogenetic inference and model choice across a large model space. . Systematic Biology 61: 539–542. [CrossRef]
    [Google Scholar]
  29. Rosa C. A. , Jindamorakot S. , Limtong S. , Nakase T. , Lachance M. A. , Fidalgo-Jiménez A. , Daniel H. M. , Pagnocca F. C. , Inácio J. , Morais P. B. . ( 2009;). Synonymy of the yeast genera Moniliella and Trichosporonoides and proposal of Moniliella fonsecae sp. nov. and five new species combinations. . Int J Syst Evol Microbiol 59: 425–429. [CrossRef] [PubMed]
    [Google Scholar]
  30. Rosa C. A. , Jindamorakot S. , Limtong S. , Nakase T. , Pagnocca F. C. , Lachance M. A. . ( 2010;). Candida golubevii sp. nov., an asexual yeast related to Metschnikowia lunata . . Int J Syst Evol Microbiol 60: 704–706. [CrossRef] [PubMed]
    [Google Scholar]
  31. Saccone C. , Lanave C. , Pesole G. , Preparata G. . ( 1990;). Influence of base composition on quantitative estimates of gene evolution. . Methods Enzymol 183: 570–583.[PubMed] [CrossRef]
    [Google Scholar]
  32. Safar S. V. , Gomes F. C. , Marques A. R. , Lachance M. A. , Rosa C. A. . ( 2013;). Kazachstania rupicola sp. nov., a yeast species isolated from water tanks of a bromeliad in Brazil. . Int J Syst Evol Microbiol 63: 1165–1168. [CrossRef] [PubMed]
    [Google Scholar]
  33. Sampaio J. P. , Gonçalves P. . ( 2008;). Natural populations of Saccharomyces kudriavzevii in Portugal are associated with oak bark and are sympatric with S . cerevisiae and S. paradoxus . . Appl Environ Microbiol 74: 2144–2152. [CrossRef] [PubMed]
    [Google Scholar]
  34. Sipiczki M. , Takeo K. , Yamaguchi M. , Yoshida S. , Miklos I. . ( 1998;). Environmentally controlled dimorphic cycle in a fission yeast. . Microbiol-UK 144: 1319–1330. [CrossRef]
    [Google Scholar]
  35. Sipiczki M. . ( 2003;). Candida zemplinina sp. nov., an osmotolerant and psychrotolerant yeast that ferments sweet botrytized wines. . Int J Syst Evol Microbiol 53: 2079–2083. [CrossRef] [PubMed]
    [Google Scholar]
  36. Sipiczki M. , Kajdacsi E. . ( 2009;). Jaminaea angkorensis gen. nov., sp. nov., a novel anamorphic fungus containing an S943 nuclear small-subunit rRNA group IB intron represents a basal branch of Microstromatales. . Int J Syst Evol Microbiol 59: 914–920. [CrossRef] [PubMed]
    [Google Scholar]
  37. Sipiczki M. . ( 2011;). Dimorphic cycle in Candida citri sp. nov., a novel yeast species isolated from rotting fruit in Borneo. . FEMS Yeast Res 11: 202–208. [CrossRef] [PubMed]
    [Google Scholar]
  38. Sipiczki M. . ( 2012;). Pichia bruneiensis sp. nov., a biofilm-producing dimorphic yeast species isolated from flowers in Borneo. . Int J Syst Evol Microbiol 62: 3099–3104. [CrossRef] [PubMed]
    [Google Scholar]
  39. Sipiczki M. . ( 2013;). Detection of yeast species also occurring in substrates associated with animals and identification of a novel dimorphic species in Verbascum flowers from Georgia. . Antonie Van Leeuwenhoek 103: 567–576. [CrossRef] [PubMed]
    [Google Scholar]
  40. Sipiczki M. , Pfliegler W. P. , Holb I. J. . ( 2013;). Metschnikowia species share a pool of diverse rRNA genes differing in regions that determine hairpin-loop structures and evolve by reticulation. . PLoS One 8: e67384. [CrossRef] [PubMed]
    [Google Scholar]
  41. Thompson J. D. , Higgins D. G. , Gibson T. J. . ( 1994;). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22: 4673–4680. [CrossRef] [PubMed]
    [Google Scholar]
  42. van der Walt J. P. , Scott D. B. , van der Klift W. C. . ( 1971;). Four new, related Candida species from South African insect sources. . Antonie Van Leeuwenhoek 37: 449–460. [CrossRef] [PubMed]
    [Google Scholar]
  43. van der Walt J. P. , Yarrow D. . ( 1984;). Methods for the isolation, maintenance, classification and identification of yeasts. . In The Yeasts, a Taxonomic Study, , 3rd edn. , pp. 45–104. Edited by Kreger-van Rij N. J. W. . Amsterdam:: Elsevier;.[CrossRef]
    [Google Scholar]
  44. van Uden N. , Kolipinski M. C. . ( 1962;). Torulopsis haemulonii nov. spec., a yeast from the Atlantic ocean. . Antonie Van Leeuwenhoek 28: 78–80. [CrossRef] [PubMed]
    [Google Scholar]
  45. White T. J. , Bruns T. , Lee S. , Taylor J. . ( 1990;). Amplification and sequencing of fungal ribosomal RNA genes for phylogenetics. . In PCR Protocols. a Guide to Methods and Applications , pp. 315–322. Edited by Innis M. A. , Gelfand D. H. , Sninsky. J. J. , White T. J. . San Diego, CA:: Acadamic Press;.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001091
Loading
/content/journal/ijsem/10.1099/ijsem.0.001091
Loading

Data & Media loading...

Supplements

Supplementary File 1



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error