RT Journal Article SR Electronic(1) A1 Guo, Xiaowei A1 Guan, Xuejiao A1 Liu, Chongxi A1 Jia, Feiyu A1 Li, jiansong A1 Li, Jinmeng A1 Jin, Pinjiao A1 Li, Wenchao A1 Wang, Xiangjing A1 Xiang, WenshengYR 2016 T1 Plantactinosporasoyae sp. nov., an endophytic actinomycete isolated from soybean root [Glycine max (L.) Merr] JF International Journal of Systematic and Evolutionary Microbiology, VO 66 IS 7 SP 2578 OP 2584 DO https://doi.org/10.1099/ijsem.0.001088 PB Microbiology Society, SN 1466-5034, AB A novel actinomycete, designated strain NEAU-gxj3T, was isolated from soybean root [Glycine max (L.) Merr.] collected from Harbin, Heilongjiang Province, China, and characterized using a polyphasic approach. The 16S rRNA gene sequence of strain NEAU-gxj3T showed highest similarity to those of Micromonospora equina Y22T (98.2 %) and Plantactinospora endophytica YIM 68255T (98.0 %). Phylogenetic analysis based on the 16S rRNA gene and gyrB gene demonstrated that the isolate clustered with the members of the genus Plantactinospora . The chemotaxonomic properties of strain NEAU-gxj3Twere also consistent with those of members of the genus Plantactinospora . The cell wall contained meso-diaminopimelic acid and whole-cell sugars were xylose, glucose and galactose. The predominant menaquinones were MK-10(H6), MK-9(H8), MK-10(H2) and MK-10(H4). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The major fatty acids were identified as anteiso-C17 : 0, iso-C16 : 0, iso-C15 : 0 and C15 : 0. A combination of DNA–DNA hybridization result and some phenotypic characteristics indicated that strain NEAU-gxj3Tcould be differentiated clearly from its closest phylogenetic relatives. Therefore, the strain is concluded to represent a novel species of the genus Plantactinospora , for which the name Plantactinospora soyae sp. nov. is proposed. The type strain is NEAU-gxj3T (=CGMCC 4.7221T=DSM 46832T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001088