%0 Journal Article %A Guo, Xiaowei %A Guan, Xuejiao %A Liu, Chongxi %A Jia, Feiyu %A Li, jiansong %A Li, Jinmeng %A Jin, Pinjiao %A Li, Wenchao %A Wang, Xiangjing %A Xiang, Wensheng %T Plantactinosporasoyae sp. nov., an endophytic actinomycete isolated from soybean root [Glycine max (L.) Merr] %D 2016 %J International Journal of Systematic and Evolutionary Microbiology, %V 66 %N 7 %P 2578-2584 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.001088 %K Polyphasic taxonomy %K 16S rRNA gene %K gyrB gene %K Plantactinospora soyae sp. nov. %K Soybean %I Microbiology Society, %X A novel actinomycete, designated strain NEAU-gxj3T, was isolated from soybean root [Glycine max (L.) Merr.] collected from Harbin, Heilongjiang Province, China, and characterized using a polyphasic approach. The 16S rRNA gene sequence of strain NEAU-gxj3T showed highest similarity to those of Micromonospora equina Y22T (98.2 %) and Plantactinospora endophytica YIM 68255T (98.0 %). Phylogenetic analysis based on the 16S rRNA gene and gyrB gene demonstrated that the isolate clustered with the members of the genus Plantactinospora . The chemotaxonomic properties of strain NEAU-gxj3Twere also consistent with those of members of the genus Plantactinospora . The cell wall contained meso-diaminopimelic acid and whole-cell sugars were xylose, glucose and galactose. The predominant menaquinones were MK-10(H6), MK-9(H8), MK-10(H2) and MK-10(H4). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The major fatty acids were identified as anteiso-C17 : 0, iso-C16 : 0, iso-C15 : 0 and C15 : 0. A combination of DNA–DNA hybridization result and some phenotypic characteristics indicated that strain NEAU-gxj3Tcould be differentiated clearly from its closest phylogenetic relatives. Therefore, the strain is concluded to represent a novel species of the genus Plantactinospora , for which the name Plantactinospora soyae sp. nov. is proposed. The type strain is NEAU-gxj3T (=CGMCC 4.7221T=DSM 46832T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001088