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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 66, Issue 7

sp. nov., an endophytic actinomycete isolated from soybean root [ (L.) Merr] Free

Abstract

A novel actinomycete, designated strain NEAU-gxj3, was isolated from soybean root [ (L.) Merr.] collected from Harbin, Heilongjiang Province, China, and characterized using a polyphasic approach. The 16S rRNA gene sequence of strain NEAU-gxj3 showed highest similarity to those of Y22 (98.2 %) and YIM 68255 (98.0 %). Phylogenetic analysis based on the 16S rRNA gene and gene demonstrated that the isolate clustered with the members of the genus . The chemotaxonomic properties of strain NEAU-gxj3were also consistent with those of members of the genus . The cell wall contained -diaminopimelic acid and whole-cell sugars were xylose, glucose and galactose. The predominant menaquinones were MK-10(H6), MK-9(H8), MK-10(H2) and MK-10(H4). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The major fatty acids were identified as anteiso-C, iso-C, iso-C and C. A combination of DNA–DNA hybridization result and some phenotypic characteristics indicated that strain NEAU-gxj3could be differentiated clearly from its closest phylogenetic relatives. Therefore, the strain is concluded to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NEAU-gxj3 (=CGMCC 4.7221=DSM 46832).

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  • Accepted:
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Keyword(s): 16S rRNA gene , gyrB gene , Plantactinospora soyae sp. nov. , Polyphasic taxonomy and Soybean
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2016-07-01
2025-03-25
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Plantactinosporasoyae sp. nov., an endophytic actinomycete isolated from soybean root [Glycine max (L.) Merr]
Int J Syst Evol Microbiol 66, 2578 (2016); https://doi.org/10.1099/ijsem.0.001088
/content/journal/ijsem/10.1099/ijsem.0.001088
/content/journal/ijsem/10.1099/ijsem.0.001088
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