1887

Abstract

A novel actinomycete, designated strain NEAU-gxj3, was isolated from soybean root [ (L.) Merr.] collected from Harbin, Heilongjiang Province, China, and characterized using a polyphasic approach. The 16S rRNA gene sequence of strain NEAU-gxj3 showed highest similarity to those of Y22 (98.2 %) and YIM 68255 (98.0 %). Phylogenetic analysis based on the 16S rRNA gene and gene demonstrated that the isolate clustered with the members of the genus . The chemotaxonomic properties of strain NEAU-gxj3were also consistent with those of members of the genus . The cell wall contained -diaminopimelic acid and whole-cell sugars were xylose, glucose and galactose. The predominant menaquinones were MK-10(H6), MK-9(H8), MK-10(H2) and MK-10(H4). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The major fatty acids were identified as anteiso-C, iso-C, iso-C and C. A combination of DNA–DNA hybridization result and some phenotypic characteristics indicated that strain NEAU-gxj3could be differentiated clearly from its closest phylogenetic relatives. Therefore, the strain is concluded to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NEAU-gxj3 (=CGMCC 4.7221=DSM 46832).

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2016-07-01
2019-12-11
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