1887

Abstract

A Gram-positive-staining, aerobic, endospore-forming bacterial strain, isolated from the stem of a cucumber plant, was studied in detail for its taxonomic position. Based on 16S rRNA gene sequence similarity comparisons, strain AP-115 was grouped into the genus , most closely related to (98.8 %), and (both 98.4 %). The 16S rRNA gene sequence similarity to other species of the genus was ≤98.4 %. Chemotaxonomic characterization supported allocation of the strain to the genus . The quinone system contained exclusively menaquinone MK-7, and in the polar lipid profile diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylserine were predominating. The major component in the polyamine pattern was spermidine, and the diagnostic diamino acid of the peptidoglycan was -diaminopimelic acid. The major fatty acids were iso- and anteiso-branched fatty acids. The results of physiological and biochemical tests allowed phenotypic differentiation of strain AP-115 from closely related species. Thus, AP-115 represents a novel species of the genus , for which the name sp. nov. is proposed, with AP-115 (=LMG 29222=CCM 8653) as the type strain.

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2016-07-01
2020-01-21
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