Dissulfurirhabdus thermomarina gen. nov., sp. nov., a thermophilic, autotrophic, sulfite-reducing and disproportionating deltaproteobacterium isolated from a shallow-sea hydrothermal vent
A thermophilic, anaerobic, chemolithoautotrophic bacterium, strain SH388T, was isolated from a shallow, submarine hydrothermal vent (Kuril Islands, Russia). Cells of strain SH388T were Gram-stain-negative short rods, 0.2–0.4 µm in diameter and 1.0–2.5 µm in length, and motile with flagella. The temperature range for growth was 25–58 °C (optimum 50 °C), and the pH range for growth was pH 5.0–7.0 (optimum pH 6.0–6.5). Growth of strain SH388T was observed in the presence of NaCl concentrations ranging from 0.5 to 4.0 % (w/v) (optimum 2.0–2.5 %). The strain grew chemolithoautotrophically with molecular hydrogen as electron donor, sodium sulfite as electron acceptor and bicarbonate/CO2 as a carbon source. It was also able to grow by disproportionation of sulfite and elemental sulfur but not thiosulfate. Sulfate, Fe(III) and nitrate were not used as electron acceptors either with H2 or organic electron donors. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belonged to the class Deltaproteobacteria and was most closely related to Dissulfuribacter thermophilus and Dissulfurimicrobium hydrothermale (91.6 % and 90.4 % sequence similarity). On the basis of its physiological properties and results of phylogenetic analyses, strain SH388T is considered to represent a novel species of a new genus, for which the name Dissulfurirhabdus thermomarina gen. nov., sp. nov. is proposed. The type strain of the species is SH388T (=DSM 100025T=VKM B-2960T). It is the first thermophilic disproportionator of sulfur compounds isolated from a shallow-sea environment.
FinsterK.,
LiesackW.,
ThamdrupB.1998; Elemental sulfur and thiosulfate disproportionation by Desulfocapsa sulfoexigens sp. nov., a new anaerobic bacterium isolated from marine surface sediment. Appl Environ Microbiol 64:119–125[PubMed]
SlobodkinA. I.,
ZavarzinaD. G.,
SokolovaT. G.,
Bonch-OsmolovskayaE. A.1999; Dissimilatory reduction of inorganic electron acceptors by thermophilic anaerobic prokaryotes. Microbiology (English Translation of Mikrobiologiia) 68:522–542
SlobodkinA. I.,
ReysenbachA. L.,
SlobodkinaG. B.,
BaslerovR. V.,
KostrikinaN. A.,
WagnerI. D.,
Bonch-OsmolovskayaE. A.2012; Thermosulfurimonas dismutans gen. nov., sp. nov., an extremely thermophilic sulfur-disproportionating bacterium from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol 62:2565–2571 [View Article][PubMed]
SlobodkinA. I.,
SlobodkinaG. B.,
PanteleevaA. N.,
ChernyhN. A.,
NovikovA. A.,
Bonch-OsmolovskayaE. A.2016; Dissulfurimicrobium hydrothermale gen. nov., sp. nov., a thermophilic, autotrophic, sulfur-disproportionating deltaproteobacterium isolated from a hydrothermal pond. Int J Syst Evol Microbiol 66:1022–1026[CrossRef]
SlobodkinaG. B.,
KolganovaT. V.,
KostrikinaN. A.,
Bonch-OsmolovskayaE. A.,
SlobodkinA. I.2012; Caloribacterium cisternae gen. nov., sp. nov., an anaerobic thermophilic bacterium from an underground gas storage reservoir. Int J Syst Evol Microbiol 62:1543–1547 [View Article][PubMed]
SorokinD. Y.,
TourovaT. P.,
HenstraA. M.,
StamsA. J.,
GalinskiE. A.,
MuyzerG.2008; Sulfidogenesis under extremely haloalkaline conditions by Desulfonatronospira thiodismutans gen. nov., sp. nov., and Desulfonatronospira delicata sp. nov. - a novel lineage of Deltaproteobacteria from hypersaline soda lakes. Microbiology 154:1444–1453 [View Article][PubMed]
SorokinD. Y.,
TourovaT. P.,
KolganovaT. V.,
DetkovaE. N.,
GalinskiE. A.,
MuyzerG.2011; Culturable diversity of lithotrophic haloalkaliphilic sulfate-reducing bacteria in soda lakes and the description of Desulfonatronum thioautotrophicum sp. nov., Desulfonatronum thiosulfatophilum sp. nov., Desulfonatronovibrio thiodismutans sp. nov., and Desulfonatronovibrio magnus sp. nov. Extremophiles 15:391–401[CrossRef]
Dissulfurirhabdus thermomarina gen. nov., sp. nov., a thermophilic, autotrophic, sulfite-reducing and disproportionating deltaproteobacterium isolated from a shallow-sea hydrothermal vent