1887

Abstract

A Gram-stain-negative, aerobic, rod-shaped, beige bacterium, strain B717-2, was isolated from an ice core drilled from Muztagh Glacier on the Tibetan Plateau, China. According to phylogenetic analyses based on 16S rRNA gene sequences, the novel strain was related most closely to Polaromonas vacuolataand shared 97.7 % similarity with the type strain of this species. It grew optimally at pH 7, at 15 °C and with 2 % (w/v) NaCl. Major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The major fatty acids were summed feature 3 (C16 : 1 ω 7 c and/or iso-C15 : 0 2-OH), summed feature 8 (C18 : 1 ω 7 c, C18 : 1 ω 6 c) and C16 : 0. The sole respiratory quinone was Q-8. The DNA G+C content was 63.4 mol %. In DNA–DNA hybridization tests, strain B717-2 shared 37.0±1.9, 30.0±1.7, 26.0±0.9, 23.4±0.5 and 18.4±1.9 % DNA–DNA relatedness with Polaromonas jejuensisJS12-13, P. vacuolata 34-P, Polaromonas aquatica CCUG 39402, Polaromonas glacialisCr4-12and Polaromonas cryoconitiCr4-35, respectively. Based on the phenotypic, phylogenetic and genetic characteristics, strain B717-2 represents a novel species of the genus Polaromonas , for which the name Polaromonas eurypsychrophila sp. nov. is proposed. The type strain is B717-2 (=CGMCC 1.15322=JCM 31171).

Keyword(s): Polaromonas and Proteobacteria
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001079
2016-07-01
2019-10-13
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/7/2497.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001079&mimeType=html&fmt=ahah

References

  1. Altenburger P. , Kämpfer P. , Makristathis A. , Lubitza W. , Bussea H.-J. . ( 1996;). Classification of bacteria isolated from a medieval wall painting. . J Biotechnology 47: 39–52. [CrossRef]
    [Google Scholar]
  2. Collins M. D. . ( 1985;). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacteria Systematics, pp. 267–287. Edited by Goodfellow. M. , Minnikin D. E. . London:: Academic Press;.
    [Google Scholar]
  3. De Ley J. , Cattoir H. , Reynaerts A. . ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12: 133–142.[PubMed] [CrossRef]
    [Google Scholar]
  4. Embley T. . ( 1991;). The linear PCR reaction: a simple and robust method for sequencing amplified rRNA genes. . Lett Appl Microbiol 13: 171–174. [CrossRef] [PubMed]
    [Google Scholar]
  5. Huss V. A. , Festl H. , Schleifer K. H. . ( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4: 184–192. [CrossRef] [PubMed]
    [Google Scholar]
  6. Irgens R. L. , Gosink J. J. , Staley J. T. . ( 1996;). Polaromonas vacuolata gen. nov., sp. nov., a psychrophilic, marine, gas vacuolate bacterium from Antarctica. . Int J Syst Bacteriol 46: 822–826. [CrossRef] [PubMed]
    [Google Scholar]
  7. Jahnke K. D. . ( 1992;). Basic computer program for evaluation of spectroscopic DNA renaturation data from Gilford System 2600 spectrophotometer on a PC/XT/AT type personal 219 computer. . J Microbiol Methods 15: 61–73.[CrossRef]
    [Google Scholar]
  8. Jeon C. O. , Park W. , Ghiorse W. C. , Madsen E. L. . ( 2004;). Polaromonas naphthalenivorans sp. nov., a naphthalene-degrading bacterium from naphthalene-contaminated sediment. . Int J Syst Evol Microbiol 54: 93–97. [CrossRef] [PubMed]
    [Google Scholar]
  9. Kämpfer P. , Busse H. , Falsen E. . ( 2006;). Polaromonas aquatica sp. nov., isolated from tap water. . Int J Syst Evol Microbiol 56: 605–608. [CrossRef] [PubMed]
    [Google Scholar]
  10. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. et al. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62: 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  11. Kim M. , Oh H. S. , Park S. C. , Chun J. . ( 2014;). Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. . Int J Syst Evol Microbiol 64: 346–351. [CrossRef] [PubMed]
    [Google Scholar]
  12. Mandel M. , Igambi L. , Bergendahl J. , Dodson M. L. , Scheltgen E. . ( 1970;). Correlation of melting temperature and cesium chloride buoyant density of bacterial deoxyribonucleic acid. . J Bacteriol 101: 333–338.[PubMed]
    [Google Scholar]
  13. Margesin R. , Spröer C. , Zhang D. C. , Busse H. J. . ( 2012;). Polaromonas glacialis sp. nov. and Polaromonas cryoconiti sp. nov., isolated from alpine glacier cryoconite. . Int J Syst Evol Microbiol 62: 2662–2668. [CrossRef] [PubMed]
    [Google Scholar]
  14. Marmur J. . ( 1961;). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. . J Mol Biol 3: 208–218.[CrossRef]
    [Google Scholar]
  15. Reasoner D. , Geldreich E. . ( 1985;). A new medium for the enumeration and subculture of bacteria from potable water. . Appl Environ Microbiol 49: 1–7.[PubMed]
    [Google Scholar]
  16. Sizova M. , Panikov N. . ( 2007;). Polaromonas hydrogenivorans sp. nov., a psychrotolerant hydrogen-oxidizing bacterium from Alaskan soil. . Int J Syst Evol Microbiol 57: 616–619. [CrossRef] [PubMed]
    [Google Scholar]
  17. Smibert R. M. , Krieg N. R. . ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P. , Murray R. G. E. , Wood. W. A. , Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  18. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011;). MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28: 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  19. Tindall B. J. . ( 1990;). Lipid composition of halobacterium lacusprofundi. . FEMS Microbiol Lett 66: 199–202. [CrossRef]
    [Google Scholar]
  20. Weon H. Y. , Yoo S. H. , Hong S. B. , Kwon S. W. , Stackebrandt E. , Go S. J. , Koo B. S. . ( 2008;). Polaromonas jejuensis sp. nov., isolated from soil in Korea. . Int J Syst Evol Microbiol 58: 1525–1528. [CrossRef] [PubMed]
    [Google Scholar]
  21. Wu C. , Lu X. , Qin M. , Wang Y. , Ruan J. . ( 1989;). Analysis of menaquinone compound in microbial cells by HPLC. . Microbiology (English Translation of Microbiologiia) 16: 176–178.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001079
Loading
/content/journal/ijsem/10.1099/ijsem.0.001079
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error