1887

Abstract

The taxonomic position of a novel actinobacterium, strain SG1, isolated from a Saharan soil sample collected from Béni-Abbès, Béchar (south-west Algeria), was established by using a polyphasic approach. The micro-organism had morphological and chemical features that were consistent with its classification in the genus . The cell-wall peptidoglycan contained -diaminopimelic acid. The whole-cell sugars contained ribose and glucose, but not madurose. The predominant menaquinones were MK-9(H) and MK-9(H). The polar lipid profile contained diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylhydroxymethylethanolamine, phosphatidylhydroxyethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. The predominant cellular fatty acids were C 8, iso-C, 10-methyl C, C 9 and C. 16S rRNA gene sequence similarity analysis supported the classification of the isolate in the genus and indicated that it was related most closely to ‘’ DSM 46724 (99.7 % similarity), DSM 43181 (98.7 %), DSM 43847 (98.6 %) and DSM 45763 (98.5 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain SG1 formed a cluster with its closest relative ‘ DSM 46724. However, DNA–DNA relatedness as well as physiological and chemotaxonomical analyses showed that strain SG1 could be differentiated from its closest phylogenetic relatives. Therefore, it is proposed that strain SG1 should be classified as representing a novel species in the genus , for which the name sp. nov. is proposed. The type strain is SG1 (=DSM 46887=CECT 8961).

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2016-07-01
2020-01-17
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References

  1. Badji B., Zitouni A., Mathieu F., Lebrihi A., Sabaou N.. 2006; Antimicrobial compounds produced by Actinomadura sp. AC104 isolated from an Algerian Saharan soil. Can J Microbiol52:373–382 [CrossRef][PubMed]
    [Google Scholar]
  2. Badji B., Mostefaoui A., Sabaou N., Lebrihi A., Mathieu F., Seguin E., Tillequin F.. 2007; Isolation and partial characterization of antimicrobial compounds from a new strain Nonomuraea sp. NM94 . J Ind Microbiol Biotechnol34:403–412 [CrossRef][PubMed]
    [Google Scholar]
  3. Becker B., Lechevalier M. P., Gordon R. E., Lechevalier H. A.. 1964; Rapid differentiation between Nocardia and Streptomyces by paper chromatography of whole-cell hydrolysates. Appl Microbiol12:421–423[PubMed]
    [Google Scholar]
  4. Boubetra D., Sabaou N., Zitouni A., Bijani C., Lebrihi A., Mathieu F.. 2012; Taxonomy and chemical characterization of new antibiotics produced by Saccharothrix SA198 isolated from a Saharan soil. Microbiol Res168:223–230 [CrossRef][PubMed]
    [Google Scholar]
  5. Boubetra D., Bouras N., Mokrane S., Zitouni A., Schumann P., Spröer C., Sabaou N., Klenk H.-P.. 2016; Streptosporangium algeriense sp. nov., an actinobacterium isolated from desert soil. Int J Syst Evol Microbiol66:1034–1038 [CrossRef]
    [Google Scholar]
  6. Boudjella H., Bouti K., Zitouni A., Mathieu F., Lebrihi A., Sabaou N.. 2006; Taxonomy and chemical characterization of antibiotics of Streptosporangium Sg 10 isolated from a Saharan soil. Microbiol Res161:288–298 [CrossRef][PubMed]
    [Google Scholar]
  7. Boudjella H., Bouti K., Zitouni A., Mathieu F., Lebrihi A., Sabaou N.. 2007; Isolation and partial characterization of pigment-like antibiotics produced by a new strain of Streptosporangium isolated from an Algerian soil. J Appl Microbiol103:228–236 [CrossRef][PubMed]
    [Google Scholar]
  8. Boudjella H., Zitouni A., Coppel Y., Mathieu F., Monje M. C., Sabaou N., Lebrihi A.. 2010; Antibiotic R2, a new angucyclinone compound from Streptosporangium sp. Sg3 . J Antibiot63:709–711
    [Google Scholar]
  9. Bouras N., Merrouche R., Lamari L., Mathieu F., Sabaou N., Lebrihi A.. 2008; Precursor-directed biosynthesis of new dithiolopyrrolone analogs by Saccharothrix algeriensis NRRL B-24137. Process Biochem43:1244–1252 [CrossRef]
    [Google Scholar]
  10. Bouras N., Meklat A., Boubetra D., Saker R., Boudjelal F., Aouiche A., Lamari L., Zitouni A., Schumann P. et al. 2014; Diversity of Actinobacteria in Algerian Saharan soil and description of sixteen new taxa. Revue ElWahat Pour Les Recherches Et Les Etudes7:67–85
    [Google Scholar]
  11. Cashion P., Holder-Franklin M. A., McCully J., Franklin M.. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem81:461–466 [CrossRef][PubMed]
    [Google Scholar]
  12. Chaabane Chaouch F., Bouras N., Mokrane S., Zitouni A., Schumann P., Spröer C., Sabaou N., Klenk H. P.. 2016; Streptosporangium saharense sp. nov., an actinobacterium isolated from Saharan soil. Int J Syst Evol Microbiol66:1371–1376 [CrossRef][PubMed]
    [Google Scholar]
  13. Cooper R., Das P., Federbush C., Mierzwa R., Patel M., Pramanik B., Truumees I.. 1990; Characterization of peptidyl-nucleoside antifungal antibiotics from fermentation broth. J Ind Microbiol5:1–8 [CrossRef][PubMed]
    [Google Scholar]
  14. Couch J. N.. 1955; A new genus and family of the Actinomycetales, with a revision of the genus Actinoplanes . J Elisha Mitchell Sci Soc71:148–155
    [Google Scholar]
  15. De Ley J., Cattoir H., Reynaerts A.. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem12:133–142 [CrossRef][PubMed]
    [Google Scholar]
  16. Fang B., Liu H., Pan T., Liu C., Guan X., He H., Yan K., Li J., Xiang W. et al. 2016; Streptosporangium lutulentum sp. nov., Streptosporangium fenghuangense sp. nov. and Streptosporangium corydalis sp. nov., three novel actinobacterial species isolated from National Forest Park of Fenghuang Mountain. Antonie Van Leeuwenhoek109:439–448 [CrossRef][PubMed]
    [Google Scholar]
  17. Felsenstein J.. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  18. Felsenstein J.. 1985; Confidence Limits on Phylogenies: an approach using the bootstrap. Evolution39:783–791 [CrossRef]
    [Google Scholar]
  19. Fitch W. M.. 1977; On the problem of discovering the most parsimonious tree. Am Nat111:223–257 [CrossRef]
    [Google Scholar]
  20. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H. N.. 1974; Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol24:54–63 [CrossRef]
    [Google Scholar]
  21. Hacène H., Boudjellal F., Lefebvre G.. 1998; AH7, a non-polyenic antifungal antibiotic produced by a new strain of Streptosporangium roseum . Microbios96:103–109[PubMed]
    [Google Scholar]
  22. Hayakawa M., Nonomura H.. 1987; Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. J Ferment Technol65:501–509 [CrossRef]
    [Google Scholar]
  23. He H. R., Zhang X. H., Wang H. Y., Liu C. X., Wang S. R., Zhao J. W., Yuan J. H., Wang X. J., Xiang W. S.. 2014; Streptosporangium nanhuense sp. nov., a novel actinomycete isolated from soil. Antonie Van Leeuwenhoek105:1025–1031 [CrossRef][PubMed]
    [Google Scholar]
  24. Huss V. A., Festl H., Schleifer K. H.. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol4:184–192 [CrossRef][PubMed]
    [Google Scholar]
  25. Inahashi Y., Iwatsuki M., Ishiyama A., Namatame M., Nishihara-Tsukashima A., Matsumoto A., Hirose T., Sunazuka T., Yamada H. et al. 2011a; Spoxazomicins A–C, novel antitrypanosomal alkaloids produced by an endophytic actinomycete, Streptosporangium oxazolinicum K07-0460T. J Antibiot64:303–307 [CrossRef]
    [Google Scholar]
  26. Inahashi Y., Matsumoto A., Ōmura S., Takahashi Y.. 2011b; Streptosporangium oxazolinicum sp. nov., a novel endophytic actinomycete producing new antitrypanosomal antibiotics, spoxazomicins. J Antibiot64:297–302 [CrossRef]
    [Google Scholar]
  27. Intra B., Matsumoto A., Inahashi Y., Ōmura S., Panbangred W., Takahashi Y., Satoshi Ōmura S.. 2014; Streptosporangium jomthongense sp. nov., an actinomycete isolated from rhizospheric soil and emendation of the genus Streptosporangium . Int J Syst Evol Microbiol64:2400–2406 [CrossRef][PubMed]
    [Google Scholar]
  28. Jukes T. H., Cantor C. R.. 1969; Evolution of protein molecules. In Mammalian Protein Metabolismvol. 3 pp.21–132 Edited by Munro H. N.. New York: Academic Press;[CrossRef]
    [Google Scholar]
  29. Kelly K. L., Judd D. B.. 1976; Color. Universal Language and Dictionary of Names (National Bureau of Standards Special Publication 440) Washington, DC: US Department of Commerce;[CrossRef]
    [Google Scholar]
  30. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  31. Kornsakulkarn J., Saepua S., Supothina S., Chanthaket R., Thongpanchang C.. 2014; Sporaridin and sporazepin from actinomycete Streptosporangium sp. BCC 24625. Phytochemistry Letters10:149–151 [CrossRef]
    [Google Scholar]
  32. Kroppenstedt R. M.. 1982; Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr5:2359–2367 [CrossRef]
    [Google Scholar]
  33. Kroppenstedt R. M.. 1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Seriesvol. 20 pp.173–179 Edited by Goodfellow M., Minnikin D. E.. London: Academic Press;
    [Google Scholar]
  34. Kämpfer P., Glaeser S. P., Grün-Wollny I., Busse H. J.. 2013; Streptosporangium sandarakinum sp. nov. Int J Syst Evol Microbiol63:2484–2489 [CrossRef][PubMed]
    [Google Scholar]
  35. Lamari L., Zitouni A., Boudjella H., Badji B., Sabaou N., Lebrihi A., Lefebvre G., Seguin E., Tillequin F.. 2002a; New dithiolopyrrolone antibiotics from Saccharothrix sp. SA 233. I. Taxonomy, fermentation, isolation and biological activities. J Antibiot55:696–701 [CrossRef]
    [Google Scholar]
  36. Lamari L., Zitouni A., Dob T., Sabaou N., Lebrihi A., Germain P., Seguin. E., Tillequin F.. 2002b; New dithiolopyrrolone antibiotics from Saccharothrix sp. SA 233. II. Physicochemical properties and structure elucidation. J Antibiot55:702–707 [CrossRef]
    [Google Scholar]
  37. Lazzarini A., Cavaletti L., Toppo G., Marinelli F.. 2000; Rare genera of actinomycetes as potential producers of new antibiotics. Antonie Van Leeuwenhoek78:399–405[PubMed][CrossRef]
    [Google Scholar]
  38. Lechevalier M. P., Lechevalier H. A.. 1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol20:435–443 [CrossRef]
    [Google Scholar]
  39. Ma Z., Liu H., Liu C., He H., Zhao J., Wang X., Li J., Wang X., Xiang W.. 2015; Streptosporangium sonchi sp. nov. and Streptosporangium kronopolitis sp. nov., two novel actinobacteria isolated from a root of common sowthistle (Sonchus oleraceus L.) and a millipede (Kronopolites svenhedind Verhoeff). Antonie Van Leeuwenhoek107:1491–1499 [CrossRef][PubMed]
    [Google Scholar]
  40. Merrouche R., Bouras N., Coppel Y., Mathieu F., Monje M. C., Sabaou N., Lebrihi A.. 2010; Dithiolopyrrolone antibiotic formation induced by adding valeric acid to the culture broth of Saccharothrix algeriensis . J Nat Prod73:1164–1166 [CrossRef][PubMed]
    [Google Scholar]
  41. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M.. 1977; Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol27:104–107 [CrossRef]
    [Google Scholar]
  42. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods2:233–241 [CrossRef]
    [Google Scholar]
  43. Nolan M., Sikorski J., Jando M., Lucas S., Lapidus A., Glavina Del Rio T., Chen F., Tice H., Pitluck S. et al. 2010; Complete genome sequence of Streptosporangium roseum type strain (NI 9100T). Stand Genomic Sci2:29–37 [CrossRef][PubMed]
    [Google Scholar]
  44. Quintana E., Goodfellow M... 2012; Genus I. Streptosporangium . In Bergey’s Manual of Systematic Bacteriology, 2nd edn.vol. 5 pp.1811–1825 Edited by Whitman W. B., Goodfellow M., Kämpfer P., Busse H. J., Trujillo M. E., Ludwig W., Suzuki K., Parte A.. New York: Springer;
    [Google Scholar]
  45. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E.. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol46:1088–1092 [CrossRef][PubMed]
    [Google Scholar]
  46. Saitou N., Nei M.. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol4:406–425[PubMed]
    [Google Scholar]
  47. Sasser M.. 1990; Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids. MIDI Technical Note 101 Newark. DE: MIDI Inc;
    [Google Scholar]
  48. Sazak A., Sahin N., Camas M., Guven K., Cetin D., Goodfellow M.. 2012; Streptosporangium anatoliense sp. nov., isolated from soil in Turkey. Antonie Van Leeuwenhoek102:269–276 [CrossRef][PubMed]
    [Google Scholar]
  49. Shirling E. B., Gottlieb D.. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol16:313–340 [CrossRef]
    [Google Scholar]
  50. Sletta H., Degnes K. F., Herfindal L., Klinkenberg G., Fjærvik E., Zahlsen K., Brunsvik A., Nygaard G., Aachmann F. L. et al. 2014; Anti-microbial and cytotoxic 1,6-dihydroxyphenazine-5,10-dioxide (iodinin) produced by Streptosporangium sp. DSM 45942 isolated from the fjord sediment. Microbiol Biotechnol 98:603–610 [CrossRef]
    [Google Scholar]
  51. Stackebrandt E., Kroppenstedt R. M., Jahnke K. D., Kemmerling C., Gurtler H.. 1994; Transfer of Streptosporangium viridogriseum (Okuda, et al. 1966), Streptosporangium viridogriseum subsp. kofuense (Nonomura and Ohara 1969), and Streptosporangium albidum (Furumai, et al. 1968) to Kutzneria gen. nov. as Kutzneria viridogrisea comb. nov., Kutzneria kofuensis comb. nov., and Kutzneria albida comb. nov., respectively, and emendation of the genus Streptosporangium . Int J Syst Bacteriol44:265–269[CrossRef]
    [Google Scholar]
  52. Takasawa S., Kawamoto I., Takahashi I., Koakura M., Okachi R., Sato S., Yamamoto M., Sato T., Nara T.. 1975; Platomycins A and B I Taxonomy of the producing strain and production, isolation and biological properties of platomycins. J Antibiot28:656–661 [CrossRef][PubMed]
    [Google Scholar]
  53. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. 2013; MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  54. Vaddavalli R., Gaddam B., Linga V. R.. 2015; Streptosporangium terrae sp. nov., a novel actinomycete isolated from the rhizosphere of Callistemon citrinus (Curtis), India. J Antibiot1–6
    [Google Scholar]
  55. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E.. International Committee on Systematic Bacteriology 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol37:463–464[CrossRef]
    [Google Scholar]
  56. Williams S. T., Goodfellow M., Alderson G.. 1989; Genus Streptomyces Waksman and Henrici 1943, 339AL . In Bergey’s Manual of Systematic Bacteriologyvol. 4 pp2452–2492 Edited by Williams S. T., Sharpe M. E., Holt J. G.. Baltimore, MD: Williams & Wilkins;
    [Google Scholar]
  57. Zhang X., Liu C., Zhang Y., Wang H., Wang S., Li C., Wang X., Xiang W.. 2014; Streptosporangium shengliense sp. nov., a novel actinomycete isolated from a lake sediment. Antonie Van Leeuwenhoek105:237–243 [CrossRef][PubMed]
    [Google Scholar]
  58. Zhou Y., Liu C., Zhang Y., Li C., Xing J., Li L., Zhou S., Wang X., Xiang W.. 2014; Streptosporangium subfuscum sp. nov., isolated from the rhizosphere of marigold (Tagetes erecta L.). Antonie Van Leeuwenhoek106:1231–1238 [CrossRef][PubMed]
    [Google Scholar]
  59. Zitouni A., Boudjella H., Mathieu F., Sabaou N., Lebrihi A.. 2004; Mutactimycin PR, a new anthracycline antibiotic from Saccharothrix sp. SA 103. I. Taxonomy, fermentation, isolation and biological activities. J Antibiot57:367–372 [CrossRef][PubMed]
    [Google Scholar]
  60. Zitouni A., Boudjella H., Lamari L., Badji B., Mathieu F., Lebrihi A., Sabaou N.. 2005; Nocardiopsis and Saccharothrix genera in Saharan soils in Algeria: isolation, biological activities and partial characterization of antibiotics. Res Microbiol156:984–993 [CrossRef][PubMed]
    [Google Scholar]
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