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Abstract

A novel Gram-stain-negative, straight or slightly curved rod-shaped, non-spore-forming, facultatively anaerobic bacterium with a single polar flagellum, designated RZB5-4, was isolated from a sample of the red algae collected from the coastal region of Rizhao, PR China (119.625° E 35.517° N). The organism grew optimally between 24 and 28 °C, at pH 7.0 and in the presence of 2–3 % (w/v) NaCl. The strain required seawater or artificial seawater for growth, and NaCl alone did not support growth. Strain RZB5-4 contained Cω7 and/or Cω6, C and iso-C as the dominant fatty acids. The respiratory quinones detected in strain RZB5-4 were ubiquinone 7, ubiquinone 8, menaquinone 7 and methylmenaquinone 7. The polar lipids of strain RZB5-4 comprised phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, one unidentified glycolipid, one unidentified phospholipid and one unknown lipid. The DNA G+C content of strain RZB5-4 was 47 mol %. Phylogenetic analysis based on 16S rRNA and gyrase B () gene sequences showed that strain RZB5-4 belonged to the genus , clustering with ATCC BAA-643. Strain RZB5-4 exhibited the highest 16S rRNA gene sequence similarity value (96.6 %) and the highest gene sequence similarity value (80.7 %), respectively, to ATCC BAA-643. On the basis of polyphasic analyses, strain RZB5-4 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RZB5-4 (=JCM 30804=KCTC 42663=MCCC 1K00697).

Keyword(s): 16S rDNA , gyrB , new taxon and Shewanella
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2016-08-01
2020-01-24
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