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Volume 66, Issue 8

sp. nov., isolated from the red algae , and emended description of Free

Abstract

A novel Gram-stain-negative, straight or slightly curved rod-shaped, non-spore-forming, facultatively anaerobic bacterium with a single polar flagellum, designated RZB5-4, was isolated from a sample of the red algae collected from the coastal region of Rizhao, PR China (119.625° E 35.517° N). The organism grew optimally between 24 and 28 °C, at pH 7.0 and in the presence of 2–3 % (w/v) NaCl. The strain required seawater or artificial seawater for growth, and NaCl alone did not support growth. Strain RZB5-4 contained Cω7 and/or Cω6, C and iso-C as the dominant fatty acids. The respiratory quinones detected in strain RZB5-4 were ubiquinone 7, ubiquinone 8, menaquinone 7 and methylmenaquinone 7. The polar lipids of strain RZB5-4 comprised phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, one unidentified glycolipid, one unidentified phospholipid and one unknown lipid. The DNA G+C content of strain RZB5-4 was 47 mol %. Phylogenetic analysis based on 16S rRNA and gyrase B () gene sequences showed that strain RZB5-4 belonged to the genus , clustering with ATCC BAA-643. Strain RZB5-4 exhibited the highest 16S rRNA gene sequence similarity value (96.6 %) and the highest gene sequence similarity value (80.7 %), respectively, to ATCC BAA-643. On the basis of polyphasic analyses, strain RZB5-4 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RZB5-4 (=JCM 30804=KCTC 42663=MCCC 1K00697).

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  • Accepted:
  • Published Online:
Keyword(s): 16S rDNA , gyrB , new taxon and Shewanella
© 2016 IUMS
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2016-08-01
2024-04-18
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References

  1. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl k. (editors) 1995 Short Protocols in Molecular Biology: A Compendium of Methods From Current Protocols in Molecular Biology, 3rd edn. New York: Wiley;
     [Google Scholar]
  2. Beveridge T. J., Lawrence J. R., Murray R. G. E. 2007; Sampling and Staining for Light Microscopy. In Methods for General and Molecular Microbiology, 3rd edn. pp. 19–33 Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  3. Bowman J. P. 2005; Genus XIII. shewanella MacDonell and Colwell 1986, 355VP (Effective Publication: MacDonell and Colwell 1985, 180). In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol. 2B pp 480–491 Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer;
     [Google Scholar]
  4. Bozal N., Montes M. J., Tudela E., Jiménez F., Guinea J. 2002; Shewanella frigidimarina and Shewanella livingstonensis sp. nov. isolated from Antarctic coastal areas. Int J Syst Evol Microbiol 52:195–205 [View Article][PubMed]
     [Google Scholar]
  5. Brettar I., Christen R., Höfle M. G. 2002; Shewanella denitrificans sp. nov., a vigorously denitrifying bacterium isolated from the oxicanoxic interface of the Gotland Deep in the central Baltic Sea. Int J Syst Evol Microbiol 52:2211–2217 [View Article][PubMed]
     [Google Scholar]
  6. Breznak J. A., Costilow R. N. 1994; Physicochemical Factors in Growth. In Methods for general and molecular bacteriology pp. 137–154 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  7. Collins M. D. 1994; Isoprenoid quinones. Chemical Methods in Prokaryotic Systematics pp 265–309 Edited by Goodfellow M., O’Donnell A. G. Chichester: Wiley;
     [Google Scholar]
  8. Hirota K., Nodasaka Y., Orikasa Y., Okuyama H., Yumoto I. 2005; Shewanella pneumatophori sp. nov., an eicosapentaenoic acid-producing marine bacterium isolated from the intestines of Pacific mackerel (Pneumatophorus japonicus) . Int J Syst Evol Microbiol 55:2355–2359 [View Article][PubMed]
     [Google Scholar]
  9. Hsu S. C., Lockwood J. L. 1975; Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. Appl Microbiol 29:422–426[PubMed]
     [Google Scholar]
  10. Ivanova E. P., Nedashkovskaya O. I., Zhukova N. V., Nicolau D. V., Christen R., Mikhailov V. V. 2003; Shewanella waksmanii sp. nov., isolated from a sipuncula (Phascolosoma japonicum) . Int J Syst Evol Microbiol 53:1471–1477 [View Article][PubMed]
     [Google Scholar]
  11. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
     [Google Scholar]
  12. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
     [Google Scholar]
  13. Lyman J., Fleming R. H. 1940; Composition of seawater. J Mar Res 3:134–146
     [Google Scholar]
  14. MacDonell M. T., Colwell R. R. 1985; Phylogeny of the Vibrionaceae, and recommendation for two new genera, Listonella and Shewanella . Syst Appl Microbiol 6:171–182 [CrossRef]
     [Google Scholar]
  15. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Evol Microbiol 39:159–167
     [Google Scholar]
  16. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
     [Google Scholar]
  17. Miyazaki M., Nogi Y., Usami R., Horikoshi K. 2006; Shewanella surugensis sp. nov., Shewanella kaireitica sp. nov. and Shewanella abyssi sp. nov., isolated from deep-sea sediments of Suruga Bay, Japan. Int J Syst Evol Microbiol 56:1607–1613 [View Article][PubMed]
     [Google Scholar]
  18. Park H. Y., Jeon C. O. 2013; Shewanella aestuarii sp. nov., a marine bacterium isolated from a tidal flat. Int J Syst Evol Microbiol 63:4683–4690 [View Article][PubMed]
     [Google Scholar]
  19. Sasser M. 1990; Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids. MIDI Technical Note 101 Newark, DE: MIDI Inc;
     [Google Scholar]
  20. Sung H. R., Yoon J. H., Ghim S. Y. 2012; Shewanella dokdonensis sp. nov., isolated from seawater. Int J Syst Evol Microbiol 62:1636–1643 [View Article][PubMed]
     [Google Scholar]
  21. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011; mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739 [View Article][PubMed]
     [Google Scholar]
  22. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
     [Google Scholar]
  23. Tindall B. J., Sikorski J., Smibert R. M., Krieg N. R. 2007; Phenotypic Characterization and the Principles of Comparative Systematics. In Methods for General and Molecular Microbiology, 3rd edn. pp. 330–393 Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R. Washington DC: American Society for Microbiology;
     [Google Scholar]
  24. Venkateswaran K., Moser D. P., Dollhopf M. E., Lies D. P., Saffarini D. A., MacGregor B. J., Ringelberg D. B., White D. C., Nishijima M. et al. 1999; Polyphasic taxonomy of the genus Shewanella and description of Shewanella oneidensis sp. nov. Int J Syst Bacteriol 49:705–724 [View Article][PubMed]
     [Google Scholar]
  25. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
     [Google Scholar]
  26. Yamamoto S., Harayama S. 1995; PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains. Appl Environ Microbiol 61:1104–1109[PubMed]
     [Google Scholar]
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Shewanella gelidii sp. nov., isolated from the red algae Gelidium amansii, and emended description of Shewanella waksmanii
Int J Syst Evol Microbiol 66, 2899 (2016); https://doi.org/10.1099/ijsem.0.001075
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