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Volume 66, Issue 8

gen. nov., sp. nov., a member of the isolated from ant guts representing a new family, fam. nov., within the order ‘’ Free

Abstract

‘turtle’ ants are known to harbor a core group of gut symbionts, including members belonging to the . Here, we describe the cultivation and characterization of strain CV58, a novel member of the order isolated from the guts of the ant . Strain CV58 was rod-shaped, Gram-stain-negative, non-motile and formed pale-yellow colonies on trypticase soy agar. Optimum growth occurred under an atmosphere of 4–20 % (v/v) O. Growth was possible for strain CV58at NaCl concentrations of 0–1.5 % (w/v), temperatures of 23–40 °C, and pH values of 5.5–8.5. The G+C content of the genomic DNA was 54.9 mol% and the major fatty acids were C ω7, C, Cω7/Cω6, C and C3OH. The only respiratory quinone detected was ubiquinone–9 (Q–9) and the major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Based on phylogenetic analysis of the 16S rRNA gene sequence, strain CV58 shared an 88.3 % nucleotide identity with its closest cultivated neighbor, R43. We believe that this, combined with the housekeeping gene phylogeny, differences in phenotypic characteristics and cellular fatty acid compositions of other cultivated members indicates that strain CV58 represents a novel species occupying a novel genus and family within the order . Thus, we propose the name fam nov., followed by gen. nov., sp. nov., to classify strain CV58 (=NCIMB 15011 =DSM 100910).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Cephalotes ants , Gammaproteobacteria , gut symbiont , Pseudomonadales and Ventosimonas
© 2016 IUMS
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2016-08-01
2024-04-18
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Ventosimonas gracilis gen. nov., sp. nov., a member of the Gammaproteobacteria isolated from Cephalotes varians ant guts representing a new family, Ventosimonadaceae fam. nov., within the order ‘Pseudomonadales’
Int J Syst Evol Microbiol 66, 2869 (2016); https://doi.org/10.1099/ijsem.0.001068
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