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Abstract

A Gram-stain-negative, rod-shaped, non-motile and aerobic bacterial strain, designated CHL1, was isolated from a sludge sample collected from a sewage treatment tank of an agricultural chemical factory. The strain grew at salinities of 0.5–5 % (w/v) NaCl (optimum 2.5 %). Growth occurred at pH 6.0–8.0 (optimum pH 7.0) and 5–40 °C (optimum 28–30 °C). The genomic DNA G+C content was determined to be 70.4 mol%. Q-10 was detected as the respiratory quinone. The major fatty acids (>10 %) were Cω7 and/or Cω6 and C. The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, two unidentified phospholipids and two unidentified aminophospholipids. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain CHL1 formed a distinct clade with DSM 22840 and DM9 within the family . On the basis of phenotypic, chemotaxonomic and phylogenetic characteristics, the strain merits recognition as a representative of a novel species of a new genus within the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is CHL1 (=KCTC 42661=CCTCC AB 2015175). In addition, the species is proposed to be transferred to the genus as comb. nov. (type strain DM9=CIP 106788=VKM B-2189) on the basis of the phylogenetic analysis. An emended description of the genus is also provided.

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2016-08-01
2020-08-13
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