@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001060, author = "Tian, Yanli and Zhao, Yuqiang and Yuan, Xiaoli and Yi, Jianping and Fan, Jiaqin and Xu, Zhigang and Hu, Baishi and De Boer, Solke H. and Li, Xiang", title = "Dickeyafangzhongdai sp. nov., a plant-pathogenic bacterium isolated from pear trees (Pyrus pyrifolia)", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2016", volume = "66", number = "8", pages = "2831-2835", doi = "https://doi.org/10.1099/ijsem.0.001060", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001060", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "pear trees", keywords = "plant-pathogenic bacterium", keywords = "new taxon", keywords = "Dickeya fangzhongdai", abstract = "Gram-stain-negative, pectinolytic bacteria were repeatedly isolated from pear trees displaying symptoms of bleeding canker in China. Three strains, JS5T, LN1 and QZH3, had identical 16S rRNA gene sequences that shared 99 % similarity to the type strain of Dickeya dadantii . Phylogenetic analysis of strains JS5T, LN1 and QZH3 with isolates representing all species of the genus Dickeya and related Pectobacterium species supported their affiliation to Dickeya . Multi-locus sequence typing employing concatenated sequences encoding recA, fusA, gapA, purA, rplB, dnaX and the intergenic spacer illustrated a phylogeny which placed strains JS5T, LN1 and QZH3 as a distinct clade, separate from all other species of the genus Dickeya . Average nucleotide identity values obtained in comparison with all species of the genus Dickeya supported the distinctiveness of strain JS5T within the genus Dickeya . Additionally, all three strains were phenotypically distinguished from other species of the genus Dickeya by failing to hydrolyse casein, and by producing acids from (−)-d-arabinose, (+)melibiose, (+)raffinose, mannitol and myo-inositol, but not from 5-keto-d-gluconate or β-gentiobiose. The name Dickeya fangzhongdai sp. nov. is proposed to accommodate these strains; the type strain is JS5T (=CGMCC 1.15464T=DSM 101947T).", }